Mineralization of 14C-diuron by Acinetobacter baumannii.

The diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] is an herbicide used in sugarcane and other crops. This work evaluated the mineralization of the herbicide diuron by Acinetobacter baumannii and also was developed protocols for extraction and quantification of 14C-diuron. The strain (D12-12), isolated from rhizosphere of sugarcane, grown in soils treated with the herbicide, was cultivated in mineral media J.E. plus diuron at 20 ug/ml and plus 14C-diuron (specific activity = 2.43 MBq/mg) applied at 39,3215 KBq/50 ml, and incubated for 32 days in duplicate. The 14C-diuron extraction was carried out on C-18 SEP-PAK columns, resuspended in scintillation cocktail and the activity determined by liquid scintillation counting. The recuperation of diuron was more than 96,2% and 14CO2 accumulated after 32 days was 1,77%.

Bibliographic Details

Main Authors:	ROQUE, M. R. A., MONTEIRO, R. T. R., FERRACINI, V. L., MELO, I. S. de
Other Authors:	M. R. A. ROQUE, EMBRAPA-CNPMA; R. T. R. MONTEIRO, CENA-USP; VERA LUCIA FERRACINI, CNPMA; ITAMAR SOARES DE MELO, CNPMA.
Format:	Separatas biblioteca
Language:	English eng
Published:	1998-05-26
Subjects:	Acinetobacter baumannii, mineralization.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/12898
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