MicroRNAs expression profiles in early responses to different levels of water deficit in Setaria viridis.

Water deficit is a major constraint for crops of economic importance in almost all agricultural regions. However, plants have an active defense system to adapt to these adverse conditions, acting in the reprogramming of gene expression responsible for encoding microRNAs (miRNAs). These miRNAs promote the regulation to the target gene expression by the post-transcriptional (PTGS) and transcriptional gene silencing (TGS), modulating several pathways including defense response to water deficit. The broader knowledge of the miRNA expression profile and its regulatory networks in response to water deficit can provide evidence for the development of new biotechnological tools for genetic improvement of several important crops. In this study, we used Setaria viridis accession A10.1 as a C4 model plant to widely investigate the miRNA expression profile in early responses to different levels of water deficit. Ecophysiological studies in Setaria viridis under water deficit and after rewatering demonstrated a drought tolerant accession, capable of a rapid recovery from the stress. Deep small RNA sequencing and degradome studies were performed in plants submitted to drought to identify differentially expressed miRNA genes and their predicted targets, using in silico analysis. Our findings showed that several miRNAs were differentially modulated in response to distinctive levels of water deficit and after rewatering. The predicted mRNA targets mainly corresponded to genes related to cell wall remodeling, antioxidant system and drought-related transcription factors, indicating that these genes are rapidly regulated in early responses to drought stress. The implications of these modulations are extensively discussed, and higher-effect miRNAs are suggested as major players for potential use in genetic engineering to improve drought tolerance in economically important crops, such as sugarcane, maize, and sorghum.

Bibliographic Details

Main Authors:	DUARTE, K E. D., BASSO, M. F., OLIVEIRA, N. G. de, SILVA, J. C. F. da, GARCIA, B. de O., DIAS, B. B. A., CARDOSO, T. B., NEPOMUCENO, A. L., KOBAYASHI, A. K., SANTIAGO, T. R., SOUZA, W. R. de, MOLINARI, H. B. C.
Other Authors:	KAROLINE ESTEFANI DUARTE, Federal University of ABC; MARCOS FERNANDO BASSO, Mato Grosso Cotton Institute; NELSON GERALDO DE OLIVEIRA, EMBRAPA AGROENERGY; JOSÉ CLEYDSON FERREIRA DA SILVA, FEDERAL UNIVERSITY OF VIÇOSA; BRUNO DE OLIVEIRA GARCIA, Federal University of Viçosa; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; THIAGO BERGAMO CARDOSO, Sempre AGTECH; ALEXANDRE LIMA NEPOMUCENO, CNPSO; ADILSON KENJI KOBAYASHI, CNPAE; THAÍS RIBEIRO SANTIAGO, UNIVERSITY OF BRASÍLIA; WAGNER RODRIGO DE SOUZA, Federal University of ABC; HUGO BRUNO CORREA MOLINARI, Sempre AGTECH.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	Physiology and Molecular Biology of Plants, v. 28, n. 8, p. 1607-1624, 2022. 2022-12-08
Subjects:	Setária, Bioenergia, Abiotic stress, Drought, Bioenergy, Setaria viridis,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1149404 https://doi.org/10.1007/s12298-022-01226-z
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