Development of an enzyme-coated microcantilever-based biosensor for specific detection of short-chain alcohols.
This paper describes the development of a biosensor designed for the enzymatic detection of short-chain alcohols. The biorecognition element, alcohol dehydrogenase, was immobilized on selfassembled monolayers deposited on top of silicon nitride microcantilevers. The self-assembly process was performed by surface activation using 3-aminopropyltriethoxysilane, followed by glutaraldehyde and biomolecule binding. X-ray photoelectron spectroscopy and atomic force microscopy were used. The biosensor showed a lower response time and sensibility from 0.03 to 1.2 mL/L. Its selectivity was analyzed through exposure to pure and mixed volatile solvents. Sensor sensibility was higher in the presence of short-chain alcohols and practically null involving other polar or nonpolar solvents.
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Artigo de periódico biblioteca |
| Language: | Ingles English |
| Published: |
2021-09-17
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| Subjects: | Biosensor, Microcantilever, Biorecognition element, |
| Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134534 https://doi.org/10.3390/I3S2021Dresden-10175 |
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