Molecular characterization of arabica and conilon coffee plants genotypes by SSR and ISSR markers.

The molecular characterization of ten genotypes of the Coffea arabica plants and of seven genotypes of C. canephora having interesting features for coffee breeding programs was carried to select the parents for breeding. A total of 40 SSR and 29 ISSR primers were used. The primers generated a total of 331 (307 polymorphic and 24 monomorphic) bands. Analysis of genetic diversity presented dissimilarity intervals ranging from 0.22 to 0.44 between the Conilon genotypes, from 0.02 to 0.28 between the Arabica genotypes, and from 0.49 to 0.60 between the genotypes of the two species in the joint analysis. Four groups were formed: I = genotypes of C. arabica, II = four progenies of C. canephora, Conilon group, and one non defined C. canephora (Conilon or Robusta), III = one progeny of un-defined C. canephora (Conilon or Robusta) and IV = one progeny of C. canephora of Robusta group. The grouping formed was consistent with the origins of each group. High stabilities of the bifurcations were found by bootstrap analysis. The use of molecular markers of the SSR and ISSR types in the diversity study was efficient in distinguishing genotypes between and within C. arabica and C. canephora.

Bibliographic Details

Main Authors:	MOTTA, L. B., SOARES, T. C. B., FERRAO, M. A. G., CAIXETA, E. T., LORENZON, R. M., SOUZA NETO, J. D. de
Other Authors:	Ludymila Brandão Motta, Universidade Federal do Espírito Santo; Taís Cristina Bastos Soares, Universidade Federal do Espírito Santo; MARIA AMELIA GAVA FERRAO, CNPCa; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa; Rodrigo Monte Lorenzon, Universidade Federal do Espírito Santo; José Dias de Souza Neto, Universidade Federal do Espírito Santo.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	2020-09-30
Subjects:	Coffea Arábica, Coffea Canephora,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1125193
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