ELISA using a recombinant chimera of ESAT-6/MPB70/MPB83 for Mycobacterium bovis diagnosis in naturally infected cattle.

Bovine tuberculosis (bTB) control programs generally rely on intradermal tuberculin tests for the antemortem diagnosis of Mycobacterium bovis infection in cattle, but these tests detect only a portion of the infected animals. The aim of the present study was to evaluate the diagnostic coverage of a combination of the bTB antemortem techniques known as the comparative intradermal tuberculin test (CITT) and an ELISA based on a recombinant chimera of ESAT-6/MPB70/MPB83 as the antigen in cattle. The results were compared to postmortem findings based on M. bovis culturing and PCR. Paired comparisons of all data (n=92) demonstrated that ELISA and LST results compared to the culturing results did not present significant differences (P=0.27 on McNemar?s test and P=0.12 on Fisher?s exact test, respectively). Using culturing as the gold standard, the sensitivity and specificity of ELISA were 79.5% (95% CI: 64.5?89.2%) and 75.5% (95% CI: 62.4?85.1%), respectively, whereas LST demonstrated 100% sensitivity (95% CI: 91.03? 100%) and 92.5% specificity (95% CI: 82.1?97.0%). The ELISA results did not reveal significant differences in relation to the LST results (P>0.99 on Fisher?s exact test). Using the latter as the gold standard, the sensitivity and specificity of ELISA were 79.1% (95% CI: 64.8?88.6%) and 79.6% (95% CI: 66.4?88.5%), respectively. The use of ELISA with the recombinant chimera of ESAT-6/MPB70/ MPB83 as the antigen complements the diagnostic coverage provided by CITT and increases the removal of infected animals from herds.

Bibliographic Details

Main Authors:	SOUZA, I. I. F. de, RODRIGUES, R. de A., JORGE, K. S. G., SILVA, M. R., LILENBAUM, W., VIDAL, C. E. S., ETGES, R. N., KOSTOVIC, M., ARAUJO, F. R.
Other Authors:	Ingrid Ieda Fernando de Souza, Universidade Federal de Mato Grosso do Sul - UFMS; Rudielle de Arruda Rodrigues, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina, Veterinaria e Zootecnia- FMVZ; Kláudia Santos Gonçalves Jorge, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina, Veterinaria e Zootecnia- FMVZ; MARCIO ROBERTO SILVA, CNPGL; Walter Lilenbaum, Universidade Federal Fluminense - UFLU/Laboratório de Bacteriologia e Veterinária; Carlos Eugênio Soto Vidal; Rodrigo Nestor Etges; Miladin Kostovic; FLABIO RIBEIRO DE ARAUJO, CNPGC.
Format:	Artigo de periódico biblioteca
Language:	English eng
Published:	2019-12-27
Subjects:	Comparative intradermal tuberculin test, Bovine tuberculosis, Mycobacterium bovis BCG,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117817
Tags:	Add Tag No Tags, Be the first to tag this record!