TnSeq mapping and BarSeq quantification of Ralstonia mutant fitness
We created transposon insertion libraries in 3 plant pathogenic Ralstonia isolates using pKMW3, a randomly barcoded Mariner transposon vector. Mutant libraries were created in R. pseudosolanacearum GMI1000, R. solanacearum IBSBF1503, and R. syzygii PSI07. (Note: The genomes for several strains are still classified as R. solanacearum, but the names here reflect the current taxonomy). See PMID: 25968644 for plasmid reference, and TnSeq / BarSeq protocols.
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| Main Authors: | , |
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| Format: | Dataset biblioteca |
| Published: |
2020
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| Subjects: | Genetics, raw sequence reads, |
| Online Access: | https://figshare.com/articles/dataset/TnSeq_mapping_and_BarSeq_quantification_of_Ralstonia_mutant_fitness/25086542 |
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