Genetic Library Construction and Screening [electronic resource] : Advanced Techniques and Applications /
PCR and cDNA Cloning Techniques -- 1 Strategies for cDNA Cling and Mapping RNA Transcripts -- 2 Cloning PCR Products -- 3 Site Directed Deletion, Insertion, and Substitution Using PCR -- 4 Long RT-PCR Cloning — Amplification of Full-Length Enterovirus Genomes -- 5 Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase -- Subtractive and Differential Display Techniques -- 6 Subtractive Hybridization and cDNA Cloning -- 7 Differential Display to Identify Steroid-Induced Genes in Endocrine Cells -- Specialized Cloning and Library Screening Strategies -- 8 Two Hyrid cDNA Cloning -- 9 Yeast One and Two Hyrid cDNA Cloning -- 10 High-Throughput Library Screening by Fluorescent Hybridisations on Gridded Membranes -- 11 Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries.
Main Authors: | , , |
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Format: | Texto biblioteca |
Language: | eng |
Published: |
Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer,
2002
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Subjects: | Life sciences., Cancer research., Biochemistry., Cell biology., Life Sciences., Cell Biology., Biochemistry, general., Cancer Research., |
Online Access: | http://dx.doi.org/10.1007/978-3-642-56408-6 |
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