From the worm in a bottle of Mezcal iDNA confirmation of a leech parasitizing the Antillean manatee
Invertebrate-derived ingested DNA (iDNA) is quickly proving to be a valuable, non-invasive tool for monitoring vertebrate species of conservation concern. Using the DNA barcoding locus, we successfully identified both the blood-feeding leech Haementeria acuecueyetzin and its blood meal-the latter is shown to be derived from the Caribbean manatee, Trichechus manatus . DNA amplification was successful despite the fact that the specimen was fixed in Mezcal (a beverage distilled from agave). We report the first confirmed case of a leech feeding on a manatee, the first record of H. acuecueyetzin for the State of Chiapas and, to our knowledge, the first case of successful DNA amplification of a biological sample fixed in Mezcal other than the caterpillar "worms" more commonly found in that beverage.
Main Authors: | , , , , |
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Format: | Texto biblioteca |
Language: | eng |
Subjects: | Trichechus manatus, Haementeria acuecueyetzin, Parásitos, Códigos de barras de ADN, Artfrosur, |
Online Access: | http://www.bioone.org/doi/10.1645/16-46?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed |
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