The effects of silver nitrate and different carbohydrate sources on somatic embryogenesis in Coffea canephora
The response of 5 Coffea canephora genotypes with regard to somatic embryogenesis was tested on media containing silver nitrate (AgNO3) and different carbohydrates (sucrose, fructose, maltose and glucose). The presence of AgNO3 caused only small modifications to the ionic equilibrium of the media. At concentrations of 30-60 ÁM, AgNO3 improved somatic embryo yield in the genotypes evaluated, while higher doses negatively affected the regenerative capacity. The substitution of maltose, glucose or fructose for sucrose produced different responses depending on the genotype. Fructose significantly increased somatic embryo production in genotypes N91 and N128, while maltose was highly effective for N75. In addition, more synchronous somatic embryo development was observed in genotype N91 when glucose was used instead of sucrose.
| Main Authors: | , , , |
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| Format: | biblioteca |
| Published: |
2000
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| Subjects: | COFFEA, COFFEA CANEPHORA, CAFE, EMBRIOGENESIS SOMATICA, FRUCTOSA, SUCROSA, MALTOSA, GLUCOSA, MEDIO DE CULTIVO, |
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