Propiedades de amilasas provenientes de la broca del cafe Hypothenemus hampei (Coleoptera: Scolytidae)
Amylase is an enzyme with high activity in the digestive system of H. hampei. Its function allows the insect to actively digest the starch present in its digestive system, providing the glucose that is required to fulfill its metabolic functions. The knowledge of the amylolytic activity from CBB is essential in the study of inhibitor selection, which can be used for transgenic coffee production. In order to determine the best extraction solution, pH and optimal temperature for the amylolytic activity of the insect, 1 g of adult insects were mixed with different solutions containing either 5 ml of water, 1 percent NaCl, or extraction buffers with different pH. Enzyme activity was determined by spectrophotometry at 580 nm. An amylolytic activity zymogram wasprepared using whole adults and adult intestines in homogeneous gels 15 percent. The best amylase extraction solution was a solution containing 1 percent NaCl. The optimal activity pH for the enzyme was 5.0 and the optimal activity temperature was 40°C. The amylolyticactivity zymogram showed several activity bands. Two main bands with enzyme activity are present in the digestive system of the CBB.
| Main Authors: | , , , |
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| Format: | biblioteca |
| Published: |
2000
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| Subjects: | COFFEA, HYPOTHENEMUS HAMPEI, SCOLYTIDAE, AMILASAS, SISTEMA DIGESTIVO, ACTIVIDAD ENZIMATICA, ENZIMAS, |
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