Construction of a molecular linkage map in coffee

A linkage map for coffee (Coffea canephora P.) totalling 1402 cM has been developed on the basis of a population of doubled haploids. Both RFLP markers and PCR-based markers (RAPD) were used to construct 15 linkage groups. Coffee genomic and cDNA clones provided the source of the probes. In total, 47 RFLP and 100 RAPD loci have been placed on the linkage map. A rather low DNA polymorphism rate (18 percent for RFLP markers and 29 percent for RAPD primers) was detected. Only 81 percent of RAPD markers and 85 percent of RFLP markers fit an expected 1:1 ratio (P greater than 0.01). The availability of a molecular linkage map has many implications for the future development of the genetics and breeding of this commercially important crop species.

Bibliographic Details

Main Authors:	102645 Paillard, M., 85180 Lashermes, P., 104742 Pétiard, V., 15687 ORSTOM, Montpellier (Francia). Laboratoire de Ressources Génétiques et Amélioration des Plantes Tropicales
Format:	biblioteca
Published:	Montpellier (Francia) 1994
Subjects:	COFFEA CANEPHORA, RAPD, RFLP, MAPAS GENETICOS, HAPLOIDIA, LOCI,
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