Formation and intracellular accumulation of caffeine and chlorogenic acid in suspension cultures of Coffea arabica
Suspension cultures of Coffea arabica produce considerable amounts of both caffeine and chlorogenic acid (5-CGA). Whereas most of the caffeine is synthesized, as reported earlier, at the end of the culture cycle, 5-CGA exhibits a biphasic formation curve with a first maximum at the beginning and a second at the end of the growth phase. In contrast to 5-CGA, which is exclusively located withing the cells, caffeine is released into the medium. However, it could be shown that caffeine is also accumulated intracellularly to a certain extent and that this is correlated with the 5-CGA concentration in the cells. Vital staining with methylene blue indicate that 5-CGA is compartmented predominantly in the vacuole.
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| Main Authors: | , |
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| Format: | biblioteca |
| Published: |
1989
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| Subjects: | COFFEA ARABICA, RUBIACEAE, CULTIVO DE TEJIDOS, CAFEINA, ACIDO CLOROGENICO, SUSPENSION CELULAR, |
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