Occurrence of 2-(4-Methoxyphenoxy) propanoic acid in roasted coffee beans: analysis by gas-liquid chromatography and by high-performance liquid chromatography
Aqueous extracts of roasted Colombian Arabica coffee beans were fractionated by solvent extraction and preparative-layer silica gel chromatography and analyzed by gas-liquid chromatography-mass spectrometry (GC-MS) equipped with BP5 or BP20 fused silica capillary colums (25 m x 0.3 mm (i.d.)) and by high-performance liquid chromatography (HPLC) equipped with a Chromspher C18 reversed-phase column (250 x 4.6 mm (i.d.)) and a diode array UV detector. The fractionated aqueous extract was shown by HPLC to contain 2-(4-methoxyphenoxy) propanoic acid. Its methyl ester, methyl 2-(4-methoxyphenoxy) propanoate, was found to be more amenable to analysis by GC-MS (greater volatility) and by HPLC (better resolution) that the acid. The purified extract was therefore methylated, and the presence of methyl 2-(4-methoxyphenoxy) propanoate was confirmed by GC-MS and HPLC analysis. The concentration of 2-(4-methoxyphenoxy) propanoic acid in roasted coffee beans was found to be 0.55-1.2 ppm
| Main Authors: | , , , , |
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| Format: | biblioteca |
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| Subjects: | CAFE TOSTADO, COMPOSICION, ACIDO PROPIONICO, COMPUESTOS ORGANICOS, ANALISIS QUIMICO, CROMATOGRAFIA LIQUIDA ALTA PRESION, |
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