Metodología de inoculacao de Corticium salmonicolor em cacaueiro
The fungus Corticium salmonicolor Berk & Broome was inoculated on cocoa seedlings in a greenhouse in an attempt to induce symptoms of pink disease under artificial conditions. Six month old Catongo, Comum and Hybrid seedlings were used. The inoculum consisted of discs of culture medium (PDA) with mycelium, basidiospores from naturally infected branches, fragments of branches colonized by the pathogen in culture medium (PDA), and fragments of bark from naturally infected branches. The inoculum was placed on the stem of the seedlings, with or without wounding, covered with wet cotton and wrapped with polyethylene tape. Seedlings inoculated with fragments of branches colonized by the fungus gave the highest rate of mycelial growth, while those inoculated with discs of mycelium and basidiospores showed only initial symptoms of the disease. Inoculations with naturally infected bark were unsuccessful. There was no difference between inoculations with and without wounds or between the three types of seedlings. Fields inoculations of Catongo trees showed that branches 1.0 to 1.5 cm in diameter, with brown bark and located inside the canopy were more susceptible. Humidity and temperature seem to be critical during disease development, which took 30-40 days under 100 per cent relative humidity and temperature below 30 centigrade grade
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| Format: | biblioteca |
| Published: |
1980
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| Subjects: | THEOBROMA CACAO, CORTICIUM SALMONICOLOR, ENFERMEDADES FUNGOSAS, INOCULACION, SINTOMAS, PODER PATOGENO, |
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