Detection of enzootic bovine leukosis with an enzyme-linked immunosorbent assay (ELISA) on pooled sera
An enzyme-linked immunosorbent assay (ELISA) involving monoclonal antibody was used to detect antibodies against bovine leukemia virus (BLV) on pooled sera grouped by herd. By this method, it is possible to detect infected herds. To detect almost all infected herds it is suggested to mix not more than 75 sera and to operate the ELISA at the serum dilution of 1/20. The use of pooled sera considerably reduces the work for large scale detection of the enzootic bovine leukosis. This method could be use as an example for the detection of other contagious animal diseases
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| Main Authors: | , , |
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| Format: | biblioteca |
| Published: |
1984
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| Subjects: | VIROSIS, LEUCOSIS BOVINA, ELISA, DIAGNOSTICO, TECNICAS INMUNOENZIMATICAS, |
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