Recombinant DNA research for viroid and virus identification
Identification of the potato spindle tuber viroid (PSTV) illustrates how recombinant DNA technology can be applied in viroid and virus research. Hybridization of highly radiactive PSTV complementary DNA to PSTV-RNA bound to a solid support, with subsequent detection of the DNA-RNA hybrids by autoradiography is more sensitive than all other methods available for PSTV diagnosis. Nonradioactive detection is also highly probable. Applying the technique to the detection of other viroids and viruses and related problems is discussed
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| Main Authors: | , , , , |
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| Format: | Texto biblioteca |
| Language: | eng |
| Published: |
Manila (Filipinas)
1985
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