Cell culture isolation and characterization of agronomically useful mutants of higher plants

Advances in the past decade in cell culture selection for agronomically useful mutants are reviewed. The mutations include resistance to herbicides or diseases and significantly increased levels of free amino acids. Early predictions concerning the requirements for successfull cell culture selection are evaluated. The significance of unexpected findings such as tissue culture-induced variability and isolation of stable cytoplasmic mutants is discussed. We describe the rescue by cell fusion of nuclear and cytoplasmic nutations, from tissue culture lines into fertile plants, a method to be applied in crops in the future. We need to improve the cell culture systems in crops and further define selection criteria in culture to modify agronomic traits

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Bibliographic Details
Main Authors: 89767 Maliga, P., 12955 IRRI, Manila (Filipinas), 35649 Inter-Center Seminar on International Agricultural Research Centers (IARCs) and Biotechnology Manila (Filipinas) 23-27 Abr 1984
Format: Texto biblioteca
Language:eng
Published: Manila (Filipinas) 1985
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