Polymorphisms in two candidate genes for beef quality in Argentina

Meat quality is a term used to describe a range of attributes of meat. It is determined by genetic and environmental factors [slaughter age, feeding and pre and post-slaughter management]. The current tendency is to study the candidate genes in order to develop molecular markers, which might be used for marker-assisted selection. The objective of this work was to evaluate the effect of polymorphisms [SNP, single nucleotide polymorphisms] in candidate genes for tenderness and fat content in steers fattened in grazing beef production systems of Argentina. Molecular methods were designed to analyze the SNP 4751 [C/T] in bovine capn1 gene [large subunit of u-calpain], associated with tenderness and two polymorphisms [exon 8:G/A and intron 9:C/T] in bovine ppargc1a gene [peroxisome proliferatoractivated receptor gamma coactivator 1-alpha] with effect on fat content in cow milk and fiber type in pigs. Information of Warner-Bratzler shear force and fat content from 60 Brangus and 21 Angus steers was used in association studies. Tenderness of cooked meat was evaluated at 1.7 and 14 days post-mortem. A large proportion of animals were heterozygotes [CT] at SNP 4751. No differences were found between genotypes of this SNP for WBSF. A low frequency of homozygote TT was found at SNP on intron 9 of the ppargc1a gene. This SNP showed no significant effect on WBSF and fat content. Two new SNPs [G/A and T/C] were identified within exon 8 of the ppargc1a gene, by multiple alignment of DNA sequences obtained from 24 bulls of different breeds [Angus, Brangus, Brahman and Braford]. One of them [G/A] could be the cause of aminoacid substitution of serine by asparagine at position 364 of the protein. The A allele was not found in Angus. The SNP T/ C is a conservative substitution. It is important that Argentina generate information about factors affecting meat quality for optimizing the production and exportation of high quality beef.

Bibliographic Details

Main Authors:	Branda Sica, A., Soria, Liliana Araceli, Corva, P. M., Villarreal, Edgardo L., Melucci, Lilia Magdalena, Mezzadra, Carlos Alberto, Schor, Alejandro, Miquel, María Cristina
Format:	Texto biblioteca
Language:	spa
Subjects:	CAPN1, FAT CONTENT, MEAT TENDERNESS, MOLECULAR MARKERS, PPARGC1A, SNP, ANIMALIA, BOVINAE, SUIDAE,
Online Access:	http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=46767
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