Chemical activation with a combination of ionomycin and dehydroleucodine for production of parthenogenetic, ICSI and cloned bovine embryos
Contents: The aim of this study was to evaluate the potential of dehydroleucodine [DhL], a new drug isolated from a medicinal herb used in Argentina, for activation of bovine oocyte. Several DhL concentrations and exposure times after ionomycin [Io] treatment were tested. The optimal DhL treatment, found for parthenogenetic development, was employed to produce bovine embryos by intracytoplasmic sperm injection [ICSI] and somatic cell nuclear transfer [SCNT]. The best parthenogenic embryo developments were observed with 5 um Io for 4 min followed by 5 um DhL concentration and after 3-h exposure time [52.3 percent cleavage; 17.4 percent morulae; 7.3 percent blastocyst; n = 109]. This treatment generated no significant differences with standard Io plus 6-dimethylaminopurine [DMAP] treatment in preimplantation embryo development. In our conditions, the embryo development reached after ICSI and SCNT assisted by the DhL treatment did not differ in terms of cleavage and blastocyst development from activation with standard Io plus DMAP treatment [p greather than 0.05]. In conclusion, DhL utilization to activate oocytes and induce development of parthenogenotes, ICSI-embryos or SCNT-embryos is reported here for first time.
| Main Authors: | , , , , , , |
|---|---|
| Format: | Texto biblioteca |
| Language: | eng |
| Subjects: | DEHYDROLEUCODINE, IONOMYCIN, LACTONE, SESQUITERPENE, ANIMAL, ANIMAL DISEASE, ANIMAL EMBRYO, CELL NUCLEUS TRANSPLANTATION, CHEMICAL STRUCTURE, CHEMISTRY, CLONING, DRUG EFFECT, EMBRYO DEVELOPMENT, INTRACYTOPLASMIC SPERM INJECTION, OOCYTE, PARTHENOGENESIS, PRENATAL DEVELOPMENT, ANIMALS, CATTLE, CLONING, ORGANISM, EMBRYO, MAMMALIAN, EMBRYONIC DEVELOPMENT, LACTONES, MOLECULAR STRUCTURE, NUCLEAR TRANSFER TECHNIQUES, OVUM, SESQUITERPENES, SPERM INJECTIONS, INTRACYTOPLASMIC, BOVINAE, |
| Online Access: | http://ceiba.agro.uba.ar/cgi-bin/koha/opac-detail.pl?biblionumber=46523 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|