Morphometric parameters and photosynthetic performance of in vitro propagated pineapple
Pineapple (Ananas comosus) is a horticultural species of the Bromeliaceae family of high socioeconomic interest, widely cultivated around the world. The multiplication of pineapple seedlings in the field can be time-consuming, requiring a significant labor investment. The objective of this study was to evaluate the performance of continuous and temporary immersion systems in the micropropagation scale-up of the species. Shoots were obtained from explants subcultured in flasks with gelled culture medium and without gas exchange. The shoots were transferred to liquid MS medium supplemented with 2mM NAA, and 4mM BAP, and cultivated in four different devices: sealed flasks, flasks with semipermeable gas membranes, RITA®, and twin-flasks. After 45 days of cultivation, plant growth, fresh mass increment, the stomatal density of the abaxial surface of the leaves, the maximum quantum yield of the photosystem II, and contents of chlorophyll and carotenoids were analyzed. Significant differences were observed in plant growth, stomatal density, and contents of chlorophyll and carotenoids. The twin-flasks and RITA® devices revealed better results in morphological parameters, such as plant growth and stomatal density, while the treatments in sealed flasks and with membrane stood out in the chlorophyll content.
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Facultad de Ciencias Agrarias y Forestales de la Universidad Nacional de La Plata
2022
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de Souza Marchi, Marcelo Sanchez Ornellas, Thiago Fritshe, Yohan Guerra, Miguel Pedro Stefenon, Valdir Marcos |
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de Souza Marchi, Marcelo Sanchez Ornellas, Thiago Fritshe, Yohan Guerra, Miguel Pedro Stefenon, Valdir Marcos Morphometric parameters and photosynthetic performance of in vitro propagated pineapple |
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de Souza Marchi, Marcelo Sanchez Ornellas, Thiago Fritshe, Yohan Guerra, Miguel Pedro Stefenon, Valdir Marcos |
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de Souza Marchi, Marcelo |
| title |
Morphometric parameters and photosynthetic performance of in vitro propagated pineapple |
| title_short |
Morphometric parameters and photosynthetic performance of in vitro propagated pineapple |
| title_full |
Morphometric parameters and photosynthetic performance of in vitro propagated pineapple |
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Morphometric parameters and photosynthetic performance of in vitro propagated pineapple |
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Morphometric parameters and photosynthetic performance of in vitro propagated pineapple |
| title_sort |
morphometric parameters and photosynthetic performance of in vitro propagated pineapple |
| description |
Pineapple (Ananas comosus) is a horticultural species of the Bromeliaceae family of high socioeconomic interest, widely cultivated around the world. The multiplication of pineapple seedlings in the field can be time-consuming, requiring a significant labor investment. The objective of this study was to evaluate the performance of continuous and temporary immersion systems in the micropropagation scale-up of the species. Shoots were obtained from explants subcultured in flasks with gelled culture medium and without gas exchange. The shoots were transferred to liquid MS medium supplemented with 2mM NAA, and 4mM BAP, and cultivated in four different devices: sealed flasks, flasks with semipermeable gas membranes, RITA®, and twin-flasks. After 45 days of cultivation, plant growth, fresh mass increment, the stomatal density of the abaxial surface of the leaves, the maximum quantum yield of the photosystem II, and contents of chlorophyll and carotenoids were analyzed. Significant differences were observed in plant growth, stomatal density, and contents of chlorophyll and carotenoids. The twin-flasks and RITA® devices revealed better results in morphological parameters, such as plant growth and stomatal density, while the treatments in sealed flasks and with membrane stood out in the chlorophyll content. |
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Facultad de Ciencias Agrarias y Forestales de la Universidad Nacional de La Plata |
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2022 |
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https://revistas.unlp.edu.ar/revagro/article/view/14060 |
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AT desouzamarchimarcelo morphometricparametersandphotosyntheticperformanceofinvitropropagatedpineapple AT sanchezornellasthiago morphometricparametersandphotosyntheticperformanceofinvitropropagatedpineapple AT fritsheyohan morphometricparametersandphotosyntheticperformanceofinvitropropagatedpineapple AT guerramiguelpedro morphometricparametersandphotosyntheticperformanceofinvitropropagatedpineapple AT stefenonvaldirmarcos morphometricparametersandphotosyntheticperformanceofinvitropropagatedpineapple AT desouzamarchimarcelo parametrosmorfometricosedesempenhofotossinteticodeabacaxipropagadoinvitro AT sanchezornellasthiago parametrosmorfometricosedesempenhofotossinteticodeabacaxipropagadoinvitro AT fritsheyohan parametrosmorfometricosedesempenhofotossinteticodeabacaxipropagadoinvitro AT guerramiguelpedro parametrosmorfometricosedesempenhofotossinteticodeabacaxipropagadoinvitro AT stefenonvaldirmarcos parametrosmorfometricosedesempenhofotossinteticodeabacaxipropagadoinvitro |
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rev-revagro-ar-article-140602022-11-29T12:53:15Z Morphometric parameters and photosynthetic performance of in vitro propagated pineapple Parâmetros morfométricos e desempenho fotossintético de abacaxi propagado in vitro de Souza Marchi, Marcelo Sanchez Ornellas, Thiago Fritshe, Yohan Guerra, Miguel Pedro Stefenon, Valdir Marcos Ananas comosus bioreactor micropropagation photosynthesis in vitro culture Ananas comosus biorreator micropropagação fotossíntese cultura in vitro Ananas comosus biorreactor micropropagación fotosíntesis cultivo in vitro Pineapple (Ananas comosus) is a horticultural species of the Bromeliaceae family of high socioeconomic interest, widely cultivated around the world. The multiplication of pineapple seedlings in the field can be time-consuming, requiring a significant labor investment. The objective of this study was to evaluate the performance of continuous and temporary immersion systems in the micropropagation scale-up of the species. Shoots were obtained from explants subcultured in flasks with gelled culture medium and without gas exchange. The shoots were transferred to liquid MS medium supplemented with 2mM NAA, and 4mM BAP, and cultivated in four different devices: sealed flasks, flasks with semipermeable gas membranes, RITA®, and twin-flasks. After 45 days of cultivation, plant growth, fresh mass increment, the stomatal density of the abaxial surface of the leaves, the maximum quantum yield of the photosystem II, and contents of chlorophyll and carotenoids were analyzed. Significant differences were observed in plant growth, stomatal density, and contents of chlorophyll and carotenoids. The twin-flasks and RITA® devices revealed better results in morphological parameters, such as plant growth and stomatal density, while the treatments in sealed flasks and with membrane stood out in the chlorophyll content. O abacaxi (Ananas comosus) é uma espécie hortícola da família Bromeliaceae de alto interesse socioeconômico, amplamente cultivada em todo o mundo. A multiplicação de mudas de abacaxi no campo pode ser demorada, exigindo um investimento significativo de mão-de-obra. O objetivo deste estudo foi avaliar o desempenho de sistemas de imersão contínua e temporária na ampliação da escala de micropropagação da espécie. As brotações foram obtidas de explantes sub-cultivados em frascos com meio de cultura gelificado e sem troca gasosa. Os brotos foram transferidos para meio líquido MS suplementado com 2mM ANA e 4mM BAP e cultivados em quatro diferentes dispositivos: frascos selados, frascos com membranas gasosas semipermeáveis, RITA® e frascos duplos. Após 45 dias de cultivo, foram analisados o crescimento das plantas, o incremento de massa fresca, a densidade estomática da superfície abaxial das folhas, o rendimento quântico máximo do fotossistema II e os teores de clorofila e carotenoides. Diferenças significativas foram observadas no crescimento das plantas, densidade estomática e teores de clorofila e carotenóides. Os frascos duplos e os dispositivos RITA® revelaram resultados superiores em parâmetros morfológicos, como crescimento vegetal e densidade estomática, enquanto os tratamentos em frascos selados e com membrana se destacaram no teor de clorofila. Facultad de Ciencias Agrarias y Forestales de la Universidad Nacional de La Plata 2022-11-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulos evaluados por pares application/pdf https://revistas.unlp.edu.ar/revagro/article/view/14060 10.24215/16699513e098 Journal of the Agronomy College; Vol. 121 No. Especial 2 (2022): Biotecnologías aplicadas a cultivos de interes socioeconómico; 098 Revista de la Facultad de Agronomía; Vol. 121 Núm. Especial 2 (2022): Biotecnologías aplicadas a cultivos de interes socioeconómico; 098 Journal de la Faculté d'Agronomie; Vol. 121 No. Especial 2 (2022): Biotecnologías aplicadas a cultivos de interes socioeconómico; 098 Revista da Faculdade de Agronomia; v. 121 n. Especial 2 (2022): Biotecnologías aplicadas a cultivos de interes socioeconómico; 098 1669-9513 0041-8676 eng https://revistas.unlp.edu.ar/revagro/article/view/14060/13385 Derechos de autor 2022 Marcelo de Souza Marchi, Thiago Sanchez Ornellas, Yohan Fritshe, Miguel Pedro Guerra, Valdir Marcos Stefenon https://creativecommons.org/licenses/by-nc-sa/4.0 |