Evaluation of soil-microbial communities by their CLPP. Standardization of a laboratory technique to replace commercial available microplates
Variation of soil-microbial communities are good bioindicators of human impacts in soils, such as different soils management or contamination. Considering that traditional methods of isolation and taxonomic analysis do not consider the functionality of the microbial community, Community-Level Physiological Profi les (CLPP) emerged as a complementary methodology to study microbial communities. Several studies have shown that Biolog® EcoPlates® are very useful for determining physiological differences between communities from different samples. However, commercial microplates have some disadvantages which led us to the idea of replacing them by microplates prepared in the laboratory (Laboratory’s). Here, we compared both types of microplates using soil samples from a bioremediation assay. We compared a) the average well color development for each sample, b) the averages of absorbance values for each type of microplate, c) Principal Components, and d) Shannon-Weaver’s diversity index (H). Although Laboratory’s showed signifi cantly lower Average absorbance values than EcoPlates®, the principal component analysis and diversity index did not differ between types of microplates. In conclusion, both types of microplates showed a relatively similar ability to detect differences in the CLPP of the treatments studied. Consequently, microplates prepared in laboratory are a reliable and economical tool to study the physiology of soil microbial communities.
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Asociación Argentina de Ecología
2012
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Di Salvo, Luciana P. García de Salamone, Inés E. |
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Di Salvo, Luciana P. García de Salamone, Inés E. Evaluation of soil-microbial communities by their CLPP. Standardization of a laboratory technique to replace commercial available microplates |
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Di Salvo, Luciana P. García de Salamone, Inés E. |
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Di Salvo, Luciana P. |
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Evaluation of soil-microbial communities by their CLPP. Standardization of a laboratory technique to replace commercial available microplates |
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Evaluation of soil-microbial communities by their CLPP. Standardization of a laboratory technique to replace commercial available microplates |
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Evaluation of soil-microbial communities by their CLPP. Standardization of a laboratory technique to replace commercial available microplates |
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Evaluation of soil-microbial communities by their CLPP. Standardization of a laboratory technique to replace commercial available microplates |
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Evaluation of soil-microbial communities by their CLPP. Standardization of a laboratory technique to replace commercial available microplates |
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evaluation of soil-microbial communities by their clpp. standardization of a laboratory technique to replace commercial available microplates |
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Variation of soil-microbial communities are good bioindicators of human impacts in soils, such as different soils management or contamination. Considering that traditional methods of isolation and taxonomic analysis do not consider the functionality of the microbial community, Community-Level Physiological Profi les (CLPP) emerged as a complementary methodology to study microbial communities. Several studies have shown that Biolog® EcoPlates® are very useful for determining physiological differences between communities from different samples. However, commercial microplates have some disadvantages which led us to the idea of replacing them by microplates prepared in the laboratory (Laboratory’s). Here, we compared both types of microplates using soil samples from a bioremediation assay. We compared a) the average well color development for each sample, b) the averages of absorbance values for each type of microplate, c) Principal Components, and d) Shannon-Weaver’s diversity index (H). Although Laboratory’s showed signifi cantly lower Average absorbance values than EcoPlates®, the principal component analysis and diversity index did not differ between types of microplates. In conclusion, both types of microplates showed a relatively similar ability to detect differences in the CLPP of the treatments studied. Consequently, microplates prepared in laboratory are a reliable and economical tool to study the physiology of soil microbial communities. |
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Asociación Argentina de Ecología |
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2012 |
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https://ojs.ecologiaaustral.com.ar/index.php/Ecologia_Austral/article/view/1256 |
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AT disalvolucianap evaluationofsoilmicrobialcommunitiesbytheirclppstandardizationofalaboratorytechniquetoreplacecommercialavailablemicroplates AT garciadesalamoneinese evaluationofsoilmicrobialcommunitiesbytheirclppstandardizationofalaboratorytechniquetoreplacecommercialavailablemicroplates AT disalvolucianap evaluaciondecomunidadesmicrobianasedaficasmedianteclppestandarizaciondeunatecnicadelaboratorioparareemplazarmicroplacascomerciales AT garciadesalamoneinese evaluaciondecomunidadesmicrobianasedaficasmedianteclppestandarizaciondeunatecnicadelaboratorioparareemplazarmicroplacascomerciales |
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rev-ecoaus-article-12562023-11-02T20:51:15Z Evaluation of soil-microbial communities by their CLPP. Standardization of a laboratory technique to replace commercial available microplates Evaluación de comunidades microbianas edáficas mediante CLPP. Estandarización de una técnica de laboratorio para reemplazar microplacas comerciales Di Salvo, Luciana P. García de Salamone, Inés E. carbon-source utilization community-level physiological profiles functional diversity metabolic profiles perfiles fisiológicos de uso de fuentes carbonadas diversidad funcional perfiles metabólicos Variation of soil-microbial communities are good bioindicators of human impacts in soils, such as different soils management or contamination. Considering that traditional methods of isolation and taxonomic analysis do not consider the functionality of the microbial community, Community-Level Physiological Profi les (CLPP) emerged as a complementary methodology to study microbial communities. Several studies have shown that Biolog® EcoPlates® are very useful for determining physiological differences between communities from different samples. However, commercial microplates have some disadvantages which led us to the idea of replacing them by microplates prepared in the laboratory (Laboratory’s). Here, we compared both types of microplates using soil samples from a bioremediation assay. We compared a) the average well color development for each sample, b) the averages of absorbance values for each type of microplate, c) Principal Components, and d) Shannon-Weaver’s diversity index (H). Although Laboratory’s showed signifi cantly lower Average absorbance values than EcoPlates®, the principal component analysis and diversity index did not differ between types of microplates. In conclusion, both types of microplates showed a relatively similar ability to detect differences in the CLPP of the treatments studied. Consequently, microplates prepared in laboratory are a reliable and economical tool to study the physiology of soil microbial communities. La variación de la composición de algunas comunidades microbianas edáficas son buenos bioindicadores del impacto de la actividad antrópica sobre los suelos, tales como diferentes formas de manejo o su contaminación. Los métodos tradicionales de aislamiento y análisis taxonómico no consideran la funcionalidad de las comunidades microbianas, por lo que los perfiles fisiológicos de uso de fuentes carbonadas (CLPP) constituyen una metodología complementaria para su estudio. Numerosos trabajos demostraron que las microplacas de Biolog® EcoPlates® son útiles para determinar diferencias fisiológicas entre comunidades de diferentes suelos. Sin embargo, estas microplacas comerciales poseen algunas desventajas, por lo cual surgió la idea de reemplazarlas por microplacas preparadas en el laboratorio. Comparamos ambos tipos de microplacas con muestras de suelo provenientes de un ensayo de biorremediación. Analizamos a) el desarrollo promedio de color para cada tratamiento, b) los valores promedio de absorbancia para cada tipo de microplaca, c) los análisis de componentes principales, y d) el índice de diversidad de Shannon-Weaver (H) para cada muestra. Si bien los valores promedio de absorbanciadifirieron significativamente entre ambos tipos de microplacas, los resultados del análisis de componentes principales y de diversidad fueron relativamente similares. En conclusión, ambos tipos de microplacas resultaron similares para detectar diferencias en los CLPP de los distintos tratamientos. Es por ello que las microplacas preparadas en el laboratorio constituyen una herramienta confiable y económica para el estudio de la fisiología de comunidades microbianas de suelo. Asociación Argentina de Ecología 2012-08-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Communications Comunicaciones application/pdf https://ojs.ecologiaaustral.com.ar/index.php/Ecologia_Austral/article/view/1256 Ecología Austral; Vol. 22 No. 2 (2012): August 2012, Pages 81-158; 129-136 Ecología Austral; Vol. 22 Núm. 2 (2012): August 2012, Pages 81-158; 129-136 1667-782X 0327-5477 eng https://ojs.ecologiaaustral.com.ar/index.php/Ecologia_Austral/article/view/1256/627 Derechos de autor 2020 Luciana P. Di Salvo, Inés E. García de Salamone https://creativecommons.org/licenses/by/3.0/ |