Cryopreservation of horse semen with a liposome and trehalose added extender

Cryopreservation of horse semen with a liposome and trehalose added extender

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The aim of the study was to evaluate the effect of cryopreserved equine semen in the presence of trehalose-loaded liposomes on the integrity and function of sperm cells. Six ejaculations of five stallions collected with an artificial vagina were used. The ejaculates were diluted with INRA 96® 2:1 v/v and transported at 22 °C to the laboratory. Before cryopreservation, the semen was diluted with INRA Freeze® to obtain the following treatments: T1) INRA Freeze® (control), T2) INRA Freeze® + liposomes, T3) INRA Freeze® + liposomes+trehalose. Data were analysed using the Kruskal Wallis test. The percentages of sperm with intact DNA were 54.5, 57.9, and 64.8% for T1, T2 and T3, respectively (P>0.05). When evaluating the acrosomal and capacitation state after filtering with Percoll®, the percentages of spermatozoa without acrosome reaction and without capacitation were 67.8, 79.2 and 68.1% in T1, T2, and T3, respectively (P>0.05), while the capacitated sperm without acrosome reaction and without capacitation was similar in T1 (47%) and T3 (32%) (P>0.05), and lower in T2 (16%) before filtering with Percoll®. The use of liposomes and liposome-trehalose did not affect on the functional status and nuclear chromatin of the equine sperm after freezing, but it did affect the percentage of capacitated sperm without acrosome reaction after selecting the thawed semen using the Percoll® gradient.

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Main Authors: Medina-León, Ariadna Z., Domínguez-Mancera, Belisario, Cazalez-Penino, Nicolás, Cervantes-Acosta, Patricia, Jácome-Sosa, Edelmira, Romero-Salas, Dora, Barrientos-Morales, Manuel
Format: Digital revista
Language:eng
Published: Faculty of Veterinary Sciences - Universidad Austral de Chile 2019
Online Access:http://revistas.uach.cl/index.php/australjvs/article/view/5914
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spelling rev-australjvs-cl-article-59142019-11-07T19:14:14Z Cryopreservation of horse semen with a liposome and trehalose added extender Cryopreservation of horse semen with a liposome and trehalose added extender Medina-León, Ariadna Z. Domínguez-Mancera, Belisario Cazalez-Penino, Nicolás Cervantes-Acosta, Patricia Jácome-Sosa, Edelmira Romero-Salas, Dora Barrientos-Morales, Manuel The aim of the study was to evaluate the effect of cryopreserved equine semen in the presence of trehalose-loaded liposomes on the integrity and function of sperm cells. Six ejaculations of five stallions collected with an artificial vagina were used. The ejaculates were diluted with INRA 96® 2:1 v/v and transported at 22 °C to the laboratory. Before cryopreservation, the semen was diluted with INRA Freeze® to obtain the following treatments: T1) INRA Freeze® (control), T2) INRA Freeze® + liposomes, T3) INRA Freeze® + liposomes+trehalose. Data were analysed using the Kruskal Wallis test. The percentages of sperm with intact DNA were 54.5, 57.9, and 64.8% for T1, T2 and T3, respectively (P>0.05). When evaluating the acrosomal and capacitation state after filtering with Percoll®, the percentages of spermatozoa without acrosome reaction and without capacitation were 67.8, 79.2 and 68.1% in T1, T2, and T3, respectively (P>0.05), while the capacitated sperm without acrosome reaction and without capacitation was similar in T1 (47%) and T3 (32%) (P>0.05), and lower in T2 (16%) before filtering with Percoll®. The use of liposomes and liposome-trehalose did not affect on the functional status and nuclear chromatin of the equine sperm after freezing, but it did affect the percentage of capacitated sperm without acrosome reaction after selecting the thawed semen using the Percoll® gradient. The aim of the study was to evaluate the effect of cryopreserved equine semen in the presence of trehalose-loaded liposomes on the integrity and function of sperm cells. Six ejaculations of five stallions collected with an artificial vagina were used. The ejaculates were diluted with INRA 96® 2:1 v/v and transported at 22 °C to the laboratory. Before cryopreservation, the semen was diluted with INRA Freeze® to obtain the following treatments: T1) INRA Freeze® (control), T2) INRA Freeze® + liposomes, T3) INRA Freeze® + liposomes+trehalose. Data were analysed using the Kruskal Wallis test. The percentages of sperm with intact DNA were 54.5, 57.9, and 64.8% for T1, T2 and T3, respectively (P>0.05). When evaluating the acrosomal and capacitation state after filtering with Percoll®, the percentages of spermatozoa without acrosome reaction and without capacitation were 67.8, 79.2 and 68.1% in T1, T2, and T3, respectively (P>0.05), while the capacitated sperm without acrosome reaction and without capacitation was similar in T1 (47%) and T3 (32%) (P>0.05), and lower in T2 (16%) before filtering with Percoll®. The use of liposomes and liposome-trehalose did not affect on the functional status and nuclear chromatin of the equine sperm after freezing, but it did affect the percentage of capacitated sperm without acrosome reaction after selecting the thawed semen using the Percoll® gradient. Faculty of Veterinary Sciences - Universidad Austral de Chile 2019-09-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf http://revistas.uach.cl/index.php/australjvs/article/view/5914 10.4067/s0719-81322019000300119 Austral Journal of Veterinary Sciences; Vol. 51 No. 3 (2019); 119-123 Austral Journal of Veterinary Sciences; Vol. 51 Núm. 3 (2019); 119-123 0719-8132 0719-8000 eng http://revistas.uach.cl/index.php/australjvs/article/view/5914/7034 Derechos de autor 2019 Austral Journal of Veterinary Sciences
institution UACH CL
collection OJS
country Chile
countrycode CL
component Revista
access En linea
databasecode rev-australjvs-cl
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Ciencias Veterinarias de la UACH
language eng
format Digital
author Medina-León, Ariadna Z.
Domínguez-Mancera, Belisario
Cazalez-Penino, Nicolás
Cervantes-Acosta, Patricia
Jácome-Sosa, Edelmira
Romero-Salas, Dora
Barrientos-Morales, Manuel
spellingShingle Medina-León, Ariadna Z.
Domínguez-Mancera, Belisario
Cazalez-Penino, Nicolás
Cervantes-Acosta, Patricia
Jácome-Sosa, Edelmira
Romero-Salas, Dora
Barrientos-Morales, Manuel
Cryopreservation of horse semen with a liposome and trehalose added extender
author_facet Medina-León, Ariadna Z.
Domínguez-Mancera, Belisario
Cazalez-Penino, Nicolás
Cervantes-Acosta, Patricia
Jácome-Sosa, Edelmira
Romero-Salas, Dora
Barrientos-Morales, Manuel
author_sort Medina-León, Ariadna Z.
title Cryopreservation of horse semen with a liposome and trehalose added extender
title_short Cryopreservation of horse semen with a liposome and trehalose added extender
title_full Cryopreservation of horse semen with a liposome and trehalose added extender
title_fullStr Cryopreservation of horse semen with a liposome and trehalose added extender
title_full_unstemmed Cryopreservation of horse semen with a liposome and trehalose added extender
title_sort cryopreservation of horse semen with a liposome and trehalose added extender
description The aim of the study was to evaluate the effect of cryopreserved equine semen in the presence of trehalose-loaded liposomes on the integrity and function of sperm cells. Six ejaculations of five stallions collected with an artificial vagina were used. The ejaculates were diluted with INRA 96® 2:1 v/v and transported at 22 °C to the laboratory. Before cryopreservation, the semen was diluted with INRA Freeze® to obtain the following treatments: T1) INRA Freeze® (control), T2) INRA Freeze® + liposomes, T3) INRA Freeze® + liposomes+trehalose. Data were analysed using the Kruskal Wallis test. The percentages of sperm with intact DNA were 54.5, 57.9, and 64.8% for T1, T2 and T3, respectively (P>0.05). When evaluating the acrosomal and capacitation state after filtering with Percoll®, the percentages of spermatozoa without acrosome reaction and without capacitation were 67.8, 79.2 and 68.1% in T1, T2, and T3, respectively (P>0.05), while the capacitated sperm without acrosome reaction and without capacitation was similar in T1 (47%) and T3 (32%) (P>0.05), and lower in T2 (16%) before filtering with Percoll®. The use of liposomes and liposome-trehalose did not affect on the functional status and nuclear chromatin of the equine sperm after freezing, but it did affect the percentage of capacitated sperm without acrosome reaction after selecting the thawed semen using the Percoll® gradient.
publisher Faculty of Veterinary Sciences - Universidad Austral de Chile
publishDate 2019
url http://revistas.uach.cl/index.php/australjvs/article/view/5914
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