Penetration of fosfomycin in HEP-2 cells and its interaction with deoxynivalenol

Penetration of fosfomycin in HEP-2 cells and its interaction with deoxynivalenol

Fosfomycin (FOS) is an antibiotic used in swine production for the treatment and prevention of resistant bacteria during the post weaning. The calcium salt form can be used in food or drinking water. Several studies showed the presence of this antibiotic in tissues and fluids such as lungs and bronchial secretions. The HEp-2 line cell is a model to study the penetration of systemically available FOS. Deoxynivalenol (DON) is a mycotoxin produced by Fusarium sp. that contaminates the raw materials and influences the performance of pigs negatively. The aim of this work was to study the penetration of FOS in cell culture lines and evaluate the interactive effect of DON on the penetration of the antibiotic in cell culture lines. The results showed that intracellular antibiotic concentrations in HEp-2 cells incubated with 130 ppm of calcium FOS oscillated between 0.4 and 1.12 mg/ml with a tmax of 8 h. When HEp-2 cells were incubated with FOS and DON, a significant variation was not observed on the cellular penetration of the antibiotic, as regard the Cmax (1.10 ppm) and tmax (12 h). It is concluded that the presence of the mycotoxin would not alter the cellular distribution of FOS in pigs.

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Main Authors: Martínez, G, Soraci, AL, Tapia, MO
Format: Digital revista
Language:spa
Published: Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata 2011
Online Access:https://revistas.unlp.edu.ar/analecta/article/view/12387
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spelling rev-analecta-ar-article-123872021-07-12T13:11:36Z Penetration of fosfomycin in HEP-2 cells and its interaction with deoxynivalenol Penetración de fosfomicina en células HEp-2 y su interacción con deoxinivalenol Martínez, G Soraci, AL Tapia, MO fosfomicina deoxinivalenol células HEp-2 cerdos fosfomycin deoxynivalenol HEp-2 cells pigs Fosfomycin (FOS) is an antibiotic used in swine production for the treatment and prevention of resistant bacteria during the post weaning. The calcium salt form can be used in food or drinking water. Several studies showed the presence of this antibiotic in tissues and fluids such as lungs and bronchial secretions. The HEp-2 line cell is a model to study the penetration of systemically available FOS. Deoxynivalenol (DON) is a mycotoxin produced by Fusarium sp. that contaminates the raw materials and influences the performance of pigs negatively. The aim of this work was to study the penetration of FOS in cell culture lines and evaluate the interactive effect of DON on the penetration of the antibiotic in cell culture lines. The results showed that intracellular antibiotic concentrations in HEp-2 cells incubated with 130 ppm of calcium FOS oscillated between 0.4 and 1.12 mg/ml with a tmax of 8 h. When HEp-2 cells were incubated with FOS and DON, a significant variation was not observed on the cellular penetration of the antibiotic, as regard the Cmax (1.10 ppm) and tmax (12 h). It is concluded that the presence of the mycotoxin would not alter the cellular distribution of FOS in pigs. Fosfomicina (FOS) es un antimicrobiano utilizado en producción porcina para prevención y tratamiento de bacterias resistentes durante el post destete. Su presentación como sal cálcica permite incorporarse en el alimento o agua de bebida. Diversos estudios evidencian la presencia del antibiótico en tejidos y fluidos como pulmón y secreciones bronquiales. La línea celular HEp-2 (células laringeas) constituye un modelo para estudiar la penetración de FOS disponible sistémicamente. Deoxinivalenol (DON) es una micotoxina producida por Fusarium sp. que contamina las materias primas e influye negativamente el rendimiento de las piaras. El objetivo del trabajo fue estudiar la penetración de FOS en líneas de cultivos celulares y evaluar el potencial efecto interactivo de DON sobre la penetración del antibiótico en líneas de cultivos celulares. Los resultados muestran que las concentraciones de antibiótico intracelular en células HEp-2 incubadas con 130 ppm de FOS cálcica, oscilaron entre 0.4 y 1.12 μg/ml con un tmax de 8 h. Cuando las células HEp-2 fueron incubadas con FOS y DON, la penetración celular del antibiótico no presentó variación significativa, en relación a la Cmax (1.10 ppm) y tmax (12 h). Se concluye que la presencia de la micotoxina no modificaría la distribución celular de FOS en cerdos. Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata 2011-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Articles Trabajos de Investigación application/pdf https://revistas.unlp.edu.ar/analecta/article/view/12387 Analecta Veterinary; Vol. 31 No. 2 (2011): Analecta Veterinaria; 23-27 Analecta Veterinaria; Vol. 31 Núm. 2 (2011): Analecta Veterinaria; 23-27 Analecta Veterinaria; Vol. 31 No. 2 (2011): Analecta Veterinaria; 23-27 Analecta Veterinaria; v. 31 n. 2 (2011): Analecta Veterinaria; 23-27 1514-2590 0365-5148 spa https://revistas.unlp.edu.ar/analecta/article/view/12387/11203
institution UNLP AR
collection OJS
country Argentina
countrycode AR
component Revista
access En linea
databasecode rev-analecta-ar
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Ciencias Veterinarias de la UNLP Argentina
language spa
format Digital
author Martínez, G
Soraci, AL
Tapia, MO
spellingShingle Martínez, G
Soraci, AL
Tapia, MO
Penetration of fosfomycin in HEP-2 cells and its interaction with deoxynivalenol
author_facet Martínez, G
Soraci, AL
Tapia, MO
author_sort Martínez, G
title Penetration of fosfomycin in HEP-2 cells and its interaction with deoxynivalenol
title_short Penetration of fosfomycin in HEP-2 cells and its interaction with deoxynivalenol
title_full Penetration of fosfomycin in HEP-2 cells and its interaction with deoxynivalenol
title_fullStr Penetration of fosfomycin in HEP-2 cells and its interaction with deoxynivalenol
title_full_unstemmed Penetration of fosfomycin in HEP-2 cells and its interaction with deoxynivalenol
title_sort penetration of fosfomycin in hep-2 cells and its interaction with deoxynivalenol
description Fosfomycin (FOS) is an antibiotic used in swine production for the treatment and prevention of resistant bacteria during the post weaning. The calcium salt form can be used in food or drinking water. Several studies showed the presence of this antibiotic in tissues and fluids such as lungs and bronchial secretions. The HEp-2 line cell is a model to study the penetration of systemically available FOS. Deoxynivalenol (DON) is a mycotoxin produced by Fusarium sp. that contaminates the raw materials and influences the performance of pigs negatively. The aim of this work was to study the penetration of FOS in cell culture lines and evaluate the interactive effect of DON on the penetration of the antibiotic in cell culture lines. The results showed that intracellular antibiotic concentrations in HEp-2 cells incubated with 130 ppm of calcium FOS oscillated between 0.4 and 1.12 mg/ml with a tmax of 8 h. When HEp-2 cells were incubated with FOS and DON, a significant variation was not observed on the cellular penetration of the antibiotic, as regard the Cmax (1.10 ppm) and tmax (12 h). It is concluded that the presence of the mycotoxin would not alter the cellular distribution of FOS in pigs.
publisher Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata
publishDate 2011
url https://revistas.unlp.edu.ar/analecta/article/view/12387
work_keys_str_mv AT martinezg penetrationoffosfomycininhep2cellsanditsinteractionwithdeoxynivalenol
AT soracial penetrationoffosfomycininhep2cellsanditsinteractionwithdeoxynivalenol
AT tapiamo penetrationoffosfomycininhep2cellsanditsinteractionwithdeoxynivalenol
AT martinezg penetraciondefosfomicinaencelulashep2ysuinteraccioncondeoxinivalenol
AT soracial penetraciondefosfomicinaencelulashep2ysuinteraccioncondeoxinivalenol
AT tapiamo penetraciondefosfomicinaencelulashep2ysuinteraccioncondeoxinivalenol
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