STANDARDIZATION OF SPECTROPHOTOMETRY FOR THE ASSESSMENT OF SPERM CONCENTRATION IN THE DOMESTIC DOG
The aims of this study were to determine the repeatability of semen measures carried out by spectrophotometry and to compare them with that of the hemocytometer, to design a standard spectrophotometric curve for canine seminal concentration, and to correlate results obtained by both methods. For these purposes, 9 ejaculates were obtained from 5 normal male dogs. Each ejaculate was divided into four parts, and then diluted 1:25, 1:50, 1:75 in distilled water; the last fraction was not diluted. All the samples were measured three consecutive times by each method. Repeatability and correlation coefficient were calculated for both methods. The coefficient of variation of the spectrophotometer and hemocytometer was 7.8% and 16.9%, respectively and the correlation between absorbance and sperm count was r=0.89 (p < 0.01). These findings further present spectrophotometry not only as a rapid and low cost method but also precise for semen evaluation in this species.
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Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata
2011
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rev-analecta-ar-article-123862021-07-12T13:23:41Z STANDARDIZATION OF SPECTROPHOTOMETRY FOR THE ASSESSMENT OF SPERM CONCENTRATION IN THE DOMESTIC DOG Estandarización de la espectrofotometría para la medición de la concentración seminal en el perro doméstico Díaz, JD Valiente, C Corrada, Y Gobello, C canino espermatozoide semen hemocitometría espectrofotometría canine spermatozoo semen hemocytometry spectrophotometry The aims of this study were to determine the repeatability of semen measures carried out by spectrophotometry and to compare them with that of the hemocytometer, to design a standard spectrophotometric curve for canine seminal concentration, and to correlate results obtained by both methods. For these purposes, 9 ejaculates were obtained from 5 normal male dogs. Each ejaculate was divided into four parts, and then diluted 1:25, 1:50, 1:75 in distilled water; the last fraction was not diluted. All the samples were measured three consecutive times by each method. Repeatability and correlation coefficient were calculated for both methods. The coefficient of variation of the spectrophotometer and hemocytometer was 7.8% and 16.9%, respectively and the correlation between absorbance and sperm count was r=0.89 (p < 0.01). These findings further present spectrophotometry not only as a rapid and low cost method but also precise for semen evaluation in this species. Los objetivos de este trabajo fueron: determinar la repetibilidad de las lecturas seminales realizadas con el espectrofotómetro y compararlas con aquellas del hemocitómetro, realizar una curva de estandarización de lectura espectrofotométrica de concentración seminal y, finalmente, correlacionar los resultados obtenidos por ambos métodos en la especie canina. Con este fin se evaluaron 9 eyaculados de 5 perros machos reproductivamente aptos. Cada uno de los eyaculados se fraccionó en cuatro partes iguales las que posteriormente se diluyeron 1:25, 1:50, y 1:75 en agua destilada, dejando una de las fracciones sin diluir. Se realizaron tres lecturas consecutivas con cada uno de los métodos. Se calculó la repetibilidad de cada método y el coeficiente de correlación entre ambos. Así, los coeficientes de variación fueron 7,8% y 16,9% para el espectrofotómetro y el hemocitómetro, respectivamente y el coeficiente de correlación entre la absorbancia y la concentración fue de r= 0,89 (p < 0.01). Estos hallazgos reivindican el uso de la espectrofotometría en la especie canina como un método no solo rápido y económico sino también con una precisión aceptable. Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata 2011-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Articles Trabajos de Investigación application/pdf https://revistas.unlp.edu.ar/analecta/article/view/12386 Analecta Veterinary; Vol. 31 No. 2 (2011): Analecta Veterinaria; 28-31 Analecta Veterinaria; Vol. 31 Núm. 2 (2011): Analecta Veterinaria; 28-31 Analecta Veterinaria; Vol. 31 No. 2 (2011): Analecta Veterinaria; 28-31 Analecta Veterinaria; v. 31 n. 2 (2011): Analecta Veterinaria; 28-31 1514-2590 0365-5148 spa https://revistas.unlp.edu.ar/analecta/article/view/12386/11204 |
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Biblioteca de la Facultad de Ciencias Veterinarias de la UNLP Argentina |
| language |
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| author |
Díaz, JD Valiente, C Corrada, Y Gobello, C |
| spellingShingle |
Díaz, JD Valiente, C Corrada, Y Gobello, C STANDARDIZATION OF SPECTROPHOTOMETRY FOR THE ASSESSMENT OF SPERM CONCENTRATION IN THE DOMESTIC DOG |
| author_facet |
Díaz, JD Valiente, C Corrada, Y Gobello, C |
| author_sort |
Díaz, JD |
| title |
STANDARDIZATION OF SPECTROPHOTOMETRY FOR THE ASSESSMENT OF SPERM CONCENTRATION IN THE DOMESTIC DOG |
| title_short |
STANDARDIZATION OF SPECTROPHOTOMETRY FOR THE ASSESSMENT OF SPERM CONCENTRATION IN THE DOMESTIC DOG |
| title_full |
STANDARDIZATION OF SPECTROPHOTOMETRY FOR THE ASSESSMENT OF SPERM CONCENTRATION IN THE DOMESTIC DOG |
| title_fullStr |
STANDARDIZATION OF SPECTROPHOTOMETRY FOR THE ASSESSMENT OF SPERM CONCENTRATION IN THE DOMESTIC DOG |
| title_full_unstemmed |
STANDARDIZATION OF SPECTROPHOTOMETRY FOR THE ASSESSMENT OF SPERM CONCENTRATION IN THE DOMESTIC DOG |
| title_sort |
standardization of spectrophotometry for the assessment of sperm concentration in the domestic dog |
| description |
The aims of this study were to determine the repeatability of semen measures carried out by spectrophotometry and to compare them with that of the hemocytometer, to design a standard spectrophotometric curve for canine seminal concentration, and to correlate results obtained by both methods. For these purposes, 9 ejaculates were obtained from 5 normal male dogs. Each ejaculate was divided into four parts, and then diluted 1:25, 1:50, 1:75 in distilled water; the last fraction was not diluted. All the samples were measured three consecutive times by each method. Repeatability and correlation coefficient were calculated for both methods. The coefficient of variation of the spectrophotometer and hemocytometer was 7.8% and 16.9%, respectively and the correlation between absorbance and sperm count was r=0.89 (p < 0.01). These findings further present spectrophotometry not only as a rapid and low cost method but also precise for semen evaluation in this species. |
| publisher |
Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata |
| publishDate |
2011 |
| url |
https://revistas.unlp.edu.ar/analecta/article/view/12386 |
| work_keys_str_mv |
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