Results from the first bimester of word at the Faculty of Veterinary Sciences- UNLP COVID-19 Diagnostic Unit: FCV-UNLP COVID-19 Diagnostic

Results from the first bimester of word at the Faculty of Veterinary Sciences- UNLP COVID-19 Diagnostic Unit: FCV-UNLP COVID-19 Diagnostic

The COVID-19 pandemic required rapid development and approval of diagnostic methods and kits. Nasal swab samples (n=1114) were derived to Faculty of Veterinary Sciences- UNLP COVID-19 diagnostic unit by the Buenos Aires Province Ministry of Health during June and July 2020, to perform molecular diagnosis of SARS- CoV-2. Samples of RNA were purified in a type II biosafety cabin and analyzed by real time RT-PCR with the GeneFinder™ kit, which amplifies 3 viral target genes (N, E y RdRp). Of the 1110 samples with positive internal control, 458 (41.2%) were positive (26.9% to all target genes, 4.2% to 2 target genes and almost 10% to one gene, mainly N). Percentage of positive samples remained similar throughout the study period, although the sample number was higher in July (781 samples; 315 positive) compared to June (333 samples; 143 positive). Samples from Berisso, Ensenada and La Plata had significantly lower positive rate than those coming from other counties (27.6% versus 60.7%, respectively). Positivity rate was higher in samples from patients with ≥3 clinical signs (55.6%). It is necessary to conduct inter-laboratory validation studies and standardization of diagnostic methods used in order to obtain reliable and reproducible results.

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Main Authors: Moré, Gastón, Panei, Carlos Javier, Fuentealba, Nadia, Aspitia, Carolina, Bravi, Maria Emilia, Origlia, Javier, Rambeaud, Magdalena, Tizzano, Marco, Rudd Garcés, Gabriela, Golijow, Carlos, Unzaga, Juan Manuel, Pecoraro, Marcelo
Format: Digital revista
Language:spa
Published: Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata 2020
Online Access:https://revistas.unlp.edu.ar/analecta/article/view/10429
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spelling rev-analecta-ar-article-104292021-04-14T14:41:46Z Results from the first bimester of word at the Faculty of Veterinary Sciences- UNLP COVID-19 Diagnostic Unit: FCV-UNLP COVID-19 Diagnostic Resultados del primer bimestre de trabajo de la unidad de diagnóstico COVID-19 de la Facultad de Ciencias Veterinarias-UNLP: Diagnóstico COVID-19 en la FCV-UNLP Moré, Gastón Panei, Carlos Javier Fuentealba, Nadia Aspitia, Carolina Bravi, Maria Emilia Origlia, Javier Rambeaud, Magdalena Tizzano, Marco Rudd Garcés, Gabriela Golijow, Carlos Unzaga, Juan Manuel Pecoraro, Marcelo SARS-CoV-2 GeneFinder™ N gene Argentina SARS-CoV-2 GeneFinder™ gen N Argentina The COVID-19 pandemic required rapid development and approval of diagnostic methods and kits. Nasal swab samples (n=1114) were derived to Faculty of Veterinary Sciences- UNLP COVID-19 diagnostic unit by the Buenos Aires Province Ministry of Health during June and July 2020, to perform molecular diagnosis of SARS- CoV-2. Samples of RNA were purified in a type II biosafety cabin and analyzed by real time RT-PCR with the GeneFinder™ kit, which amplifies 3 viral target genes (N, E y RdRp). Of the 1110 samples with positive internal control, 458 (41.2%) were positive (26.9% to all target genes, 4.2% to 2 target genes and almost 10% to one gene, mainly N). Percentage of positive samples remained similar throughout the study period, although the sample number was higher in July (781 samples; 315 positive) compared to June (333 samples; 143 positive). Samples from Berisso, Ensenada and La Plata had significantly lower positive rate than those coming from other counties (27.6% versus 60.7%, respectively). Positivity rate was higher in samples from patients with ≥3 clinical signs (55.6%). It is necessary to conduct inter-laboratory validation studies and standardization of diagnostic methods used in order to obtain reliable and reproducible results. La pandemia de la COVID-19 planteó el rápido diseño y aprobación excepcional de diversos métodos de diagnóstico. En la unidad de diagnóstico COVID- 19 de la FCV-UNLP, se realizó el diagnóstico molecular de la presencia de SARS-CoV-2 en 1114 hisopados de pacientes derivados por el Ministerio de Salud de la Provincia de Buenos Aires. Las muestras de ARN fueron purificadas en cabina de seguridad y se analizaron mediante real time RT-PCR con el kit GeneFinder™ para tres targets virales (N, E y RdRp). De 1110 muestras con reacción al control interno, 458 (41,2%) fueron reactivas, 26,9% a tres targets virales, 4,2% a dos y cerca del 10% a uno (principalmente N). El porcentaje de positividad fue similar en el tiempo, aunque la cantidad fue mayor en julio (781 muestras; 315 reactivas) respecto a junio (333 muestras; 143 reactivas). Las muestras de Berisso, Ensenada y La Plata presentaron un porcentaje de positividad significativamente menor al de los demás municipios (27,6% vs. 60,7%). Las muestras de pacientes con tres o más signos relevantes presentaron una mayor positividad (55,6%) y menor reactividad a un único target. Es necesaria la validación interlaboratorios y la estandarización de los métodos para brindar resultados confiables y reproducibles. Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata 2020-10-28 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Articles Trabajos de Investigación application/pdf application/zip text/html https://revistas.unlp.edu.ar/analecta/article/view/10429 10.24215/15142590e050 Analecta Veterinary; Vol. 40 No. 2 (2020): Analecta Veterinaria; 050 Analecta Veterinaria; Vol. 40 Núm. 2 (2020): Analecta Veterinaria; 050 Analecta Veterinaria; Vol. 40 No. 2 (2020): Analecta Veterinaria; 050 Analecta Veterinaria; v. 40 n. 2 (2020): Analecta Veterinaria; 050 1514-2590 0365-5148 spa https://revistas.unlp.edu.ar/analecta/article/view/10429/9299 https://revistas.unlp.edu.ar/analecta/article/view/10429/9300 https://revistas.unlp.edu.ar/analecta/article/view/10429/9301 Derechos de autor 2020 Gastón Moré, Carlos Javier Panei, Nadia Fuentealba, Magdalena Rambeaud, Juan Manuel Unzaga
institution UNLP AR
collection OJS
country Argentina
countrycode AR
component Revista
access En linea
databasecode rev-analecta-ar
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Ciencias Veterinarias de la UNLP Argentina
language spa
format Digital
author Moré, Gastón
Panei, Carlos Javier
Fuentealba, Nadia
Aspitia, Carolina
Bravi, Maria Emilia
Origlia, Javier
Rambeaud, Magdalena
Tizzano, Marco
Rudd Garcés, Gabriela
Golijow, Carlos
Unzaga, Juan Manuel
Pecoraro, Marcelo
spellingShingle Moré, Gastón
Panei, Carlos Javier
Fuentealba, Nadia
Aspitia, Carolina
Bravi, Maria Emilia
Origlia, Javier
Rambeaud, Magdalena
Tizzano, Marco
Rudd Garcés, Gabriela
Golijow, Carlos
Unzaga, Juan Manuel
Pecoraro, Marcelo
Results from the first bimester of word at the Faculty of Veterinary Sciences- UNLP COVID-19 Diagnostic Unit: FCV-UNLP COVID-19 Diagnostic
author_facet Moré, Gastón
Panei, Carlos Javier
Fuentealba, Nadia
Aspitia, Carolina
Bravi, Maria Emilia
Origlia, Javier
Rambeaud, Magdalena
Tizzano, Marco
Rudd Garcés, Gabriela
Golijow, Carlos
Unzaga, Juan Manuel
Pecoraro, Marcelo
author_sort Moré, Gastón
title Results from the first bimester of word at the Faculty of Veterinary Sciences- UNLP COVID-19 Diagnostic Unit: FCV-UNLP COVID-19 Diagnostic
title_short Results from the first bimester of word at the Faculty of Veterinary Sciences- UNLP COVID-19 Diagnostic Unit: FCV-UNLP COVID-19 Diagnostic
title_full Results from the first bimester of word at the Faculty of Veterinary Sciences- UNLP COVID-19 Diagnostic Unit: FCV-UNLP COVID-19 Diagnostic
title_fullStr Results from the first bimester of word at the Faculty of Veterinary Sciences- UNLP COVID-19 Diagnostic Unit: FCV-UNLP COVID-19 Diagnostic
title_full_unstemmed Results from the first bimester of word at the Faculty of Veterinary Sciences- UNLP COVID-19 Diagnostic Unit: FCV-UNLP COVID-19 Diagnostic
title_sort results from the first bimester of word at the faculty of veterinary sciences- unlp covid-19 diagnostic unit: fcv-unlp covid-19 diagnostic
description The COVID-19 pandemic required rapid development and approval of diagnostic methods and kits. Nasal swab samples (n=1114) were derived to Faculty of Veterinary Sciences- UNLP COVID-19 diagnostic unit by the Buenos Aires Province Ministry of Health during June and July 2020, to perform molecular diagnosis of SARS- CoV-2. Samples of RNA were purified in a type II biosafety cabin and analyzed by real time RT-PCR with the GeneFinder™ kit, which amplifies 3 viral target genes (N, E y RdRp). Of the 1110 samples with positive internal control, 458 (41.2%) were positive (26.9% to all target genes, 4.2% to 2 target genes and almost 10% to one gene, mainly N). Percentage of positive samples remained similar throughout the study period, although the sample number was higher in July (781 samples; 315 positive) compared to June (333 samples; 143 positive). Samples from Berisso, Ensenada and La Plata had significantly lower positive rate than those coming from other counties (27.6% versus 60.7%, respectively). Positivity rate was higher in samples from patients with ≥3 clinical signs (55.6%). It is necessary to conduct inter-laboratory validation studies and standardization of diagnostic methods used in order to obtain reliable and reproducible results.
publisher Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata
publishDate 2020
url https://revistas.unlp.edu.ar/analecta/article/view/10429
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