Random amplified microsatellites (RAM´s) in plant genetic diversity studies

The use and importance, advantages, disadvantages and features of the Random Amplified Microsatellites RAMs technique, were reviewed in Cape gooseberry Physalis peruviana, blackberry Rubus spp, guava Psidium guajava and heliconias Heliconia spp. In blackberry, we differentiated the species R. glaucus, R. robustus y R. urticifolius, detected duplicated accessions and found high genetic diversity in R. glaucus, the most important specie. In cape gooseberry we found high diversity and two red fruit accessions genetically differentiated from the yellow fruit ones and a geographical region with high variability. In guava, primers were highly polymorphic and found high variability in Valle del Cauca region. In Heliconia and related species we differentiated families belonging to Zingiberal order, between some sub genera and variation among specie. The technique has low cost of implementation, use a single primer, do not require previous information, is highly polymorphic and can differentiate species is taxa were technique was evaluated.

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Auteurs principaux: Muñoz Flórez, Jaime Eduardo, Morillo Coronado, Ana Cruz, Morillo Coronado, Yacenia
Format: Digital revista
Langue:spa
Publié: Universidad Nacional de Colombia - Sede Palmira 2008
Accès en ligne:https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/9255
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