Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics
Maize (Zea mays) hybrid breeding programs are increasingly adopting double haploid (DH) technology to enhance efficiency and meet the growing demand for food in the face of population growth and climate change. However, in developing countries such as those in subtropical regions, the utilization of DH technology is still limited. This study aims to evaluate the potential benefits of incorporating this technology into both new and established maize breeding programs. Using a pedigree selfing population from the CIMMYT Genebank, DH lines were developed and compared to the conventional pedigree selfing method through a line-by-tester evaluation. Fifteen DH lines and fourteen lines from each of the S2, S4, and S6 selfing generations were subjected to multilocation yield trials in the subtropics of Mexico. The results showed that it took three years for the S1 population to identify DH lines with 100 % homozygosity and the highest general combining ability (GCA), which was one year earlier than the S6 lines (98,4 % homozygous). DH lines exhibited more genotype-by-environment interactions (GxE) compared to S2 testcrosses but demonstrated similar heritability to the S6 generation. Significant statistical differences were observed between the DH evaluations and the S4 and S6 testcrosses, while no significant difference was found between the S2 and DH evaluations. Based on these findings, a gradual implementation of DH technology in hybrid breeding programs is recommended. However, the conventional pedigree method should not be abandoned entirely, and testing in selfing generations, particularly in S6, remains crucial. This study provides a statistical comparison of DH versus the conventional pedigree method using a line-by-tester approach, contributing valuable insights for plant breeders and researchers in the field of maize breeding.
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Universidad Nacional de Colombia - Sede Palmira
2024
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Vargas Escobar, Ever Andrés García Dávila, Mario Augusto |
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Vargas Escobar, Ever Andrés García Dávila, Mario Augusto Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics |
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Vargas Escobar, Ever Andrés García Dávila, Mario Augusto |
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Vargas Escobar, Ever Andrés |
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Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics |
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Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics |
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Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics |
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Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics |
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Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics |
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comparison of maize performance between doubled haploids and different selfing generations in the mexican subtropics |
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Maize (Zea mays) hybrid breeding programs are increasingly adopting double haploid (DH) technology to enhance efficiency and meet the growing demand for food in the face of population growth and climate change. However, in developing countries such as those in subtropical regions, the utilization of DH technology is still limited. This study aims to evaluate the potential benefits of incorporating this technology into both new and established maize breeding programs. Using a pedigree selfing population from the CIMMYT Genebank, DH lines were developed and compared to the conventional pedigree selfing method through a line-by-tester evaluation. Fifteen DH lines and fourteen lines from each of the S2, S4, and S6 selfing generations were subjected to multilocation yield trials in the subtropics of Mexico. The results showed that it took three years for the S1 population to identify DH lines with 100 % homozygosity and the highest general combining ability (GCA), which was one year earlier than the S6 lines (98,4 % homozygous). DH lines exhibited more genotype-by-environment interactions (GxE) compared to S2 testcrosses but demonstrated similar heritability to the S6 generation. Significant statistical differences were observed between the DH evaluations and the S4 and S6 testcrosses, while no significant difference was found between the S2 and DH evaluations. Based on these findings, a gradual implementation of DH technology in hybrid breeding programs is recommended. However, the conventional pedigree method should not be abandoned entirely, and testing in selfing generations, particularly in S6, remains crucial. This study provides a statistical comparison of DH versus the conventional pedigree method using a line-by-tester approach, contributing valuable insights for plant breeders and researchers in the field of maize breeding. |
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Universidad Nacional de Colombia - Sede Palmira |
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2024 |
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https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/85349 |
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AT vargasescobareverandres comparisonofmaizeperformancebetweendoubledhaploidsanddifferentselfinggenerationsinthemexicansubtropics AT garciadavilamarioaugusto comparisonofmaizeperformancebetweendoubledhaploidsanddifferentselfinggenerationsinthemexicansubtropics AT vargasescobareverandres comparaciondelrendimientodelmaizentredobleshaploidesydiferentesgeneracionesdeautofecundacionenelsubtropicomexicano AT garciadavilamarioaugusto comparaciondelrendimientodelmaizentredobleshaploidesydiferentesgeneracionesdeautofecundacionenelsubtropicomexicano |
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oai:www.revistas.unal.edu.co:article-853492024-04-03T20:41:07Z Comparison of maize performance between doubled haploids and different selfing generations in the Mexican subtropics Comparación del rendimiento del maíz entre dobles haploides y diferentes generaciones de autofecundación en el subtrópico mexicano Vargas Escobar, Ever Andrés García Dávila, Mario Augusto double haploid homozygosis line by tester maize yield trails Plant breeding homocigosis línea por probador maíz pruebas de rendimiento Fitomejoramiento Maize (Zea mays) hybrid breeding programs are increasingly adopting double haploid (DH) technology to enhance efficiency and meet the growing demand for food in the face of population growth and climate change. However, in developing countries such as those in subtropical regions, the utilization of DH technology is still limited. This study aims to evaluate the potential benefits of incorporating this technology into both new and established maize breeding programs. Using a pedigree selfing population from the CIMMYT Genebank, DH lines were developed and compared to the conventional pedigree selfing method through a line-by-tester evaluation. Fifteen DH lines and fourteen lines from each of the S2, S4, and S6 selfing generations were subjected to multilocation yield trials in the subtropics of Mexico. The results showed that it took three years for the S1 population to identify DH lines with 100 % homozygosity and the highest general combining ability (GCA), which was one year earlier than the S6 lines (98,4 % homozygous). DH lines exhibited more genotype-by-environment interactions (GxE) compared to S2 testcrosses but demonstrated similar heritability to the S6 generation. Significant statistical differences were observed between the DH evaluations and the S4 and S6 testcrosses, while no significant difference was found between the S2 and DH evaluations. Based on these findings, a gradual implementation of DH technology in hybrid breeding programs is recommended. However, the conventional pedigree method should not be abandoned entirely, and testing in selfing generations, particularly in S6, remains crucial. This study provides a statistical comparison of DH versus the conventional pedigree method using a line-by-tester approach, contributing valuable insights for plant breeders and researchers in the field of maize breeding. Los programas de fitomejoramiento de maíz (Zea mays) híbrido están adoptando cada vez más la tecnología de dobles haploides (DH) para mejorar la eficiencia y satisfacer la creciente demanda de alimentos en el contexto del crecimiento de la población y el cambio climático. Sin embargo, en países en desarrollo, como aquellos en regiones subtropicales, la utilización de la tecnología DH aún es limitada. Este estudio tiene como objetivo evaluar los posibles beneficios de incorporar la tecnología DH en programas de mejoramiento de maíz, tanto nuevos como establecidos. Utilizando una población de autofecundación de pedigrí proveniente del Banco de Germoplasma CIMMYT, se desarrollaron líneas DH y se compararon con el método convencional de autofecundación de pedigrí a través de una evaluación línea por probador. Quince líneas DH y catorce líneas de cada una de las generaciones de autofecundación S2, S4 y S6 se sometieron a ensayos de rendimiento en múltiples localidades de la región subtropical de México. Los resultados mostraron que se necesitaron tres años en la población S1 para identificar líneas DH con 100 % de homocigosidad y la mayor habilidad combinatoria general (HCG), lo cual ocurrió un año antes que las líneas S6 (98,4 % homocigotas). Las líneas DH mostraron más interacciones genotipo por ambiente (GxE) en comparación con los híbridos S2, pero demostraron una heredabilidad similar a la generación S6. Se encontraron diferencias estadísticamente significativas entre las evaluaciones DH y los híbridos S4 y S6, mientras que no se encontraron diferencias significativas entre las evaluaciones S2 y DH. Con base en estos hallazgos, se recomienda una implementación gradual de la tecnología DH en programas de mejoramiento de híbridos. Sin embargo, el método convencional de pedigrí no debe ser abandonado por completo y las pruebas en generaciones de autofecundación, especialmente en S6, siguen siendo fundamentales. Este estudio proporciona una comparación estadística de DH versus el método convencional de pedigrí utilizando un enfoque línea por probador, aportando ideas valiosas para los fitomejoradores de plantas e investigadores en el campo del mejoramiento del maíz. Universidad Nacional de Colombia - Sede Palmira 2024-03-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion experimental experimental application/pdf https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/85349 10.15446/acag.v72n2.85349 Acta Agronómica; Vol. 72 No. 2 (2023); 168-175 Acta Agronómica; Vol. 72 Núm. 2 (2023); 168-175 Acta Agronómica; v. 72 n. 2 (2023); 168-175 2323-0118 0120-2812 eng https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/85349/91424 Worldwide Mundial Derechos de autor 2024 Acta Agronómica https://creativecommons.org/licenses/by-nc-nd/4.0 |