Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz)

Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz)

Transient expression is a common technique used for the co-expression of proteins for a wide range of experiments in molecular biology. This technique has been frequently used for gene study in plant pathogen interaction and has proved to be versatile and effective. However, there are still few reports and sparse data for transient expression in cassava leaves from adult/mature plants. For this reason, in this study we evaluated some regards of transient expression for the gene GUS in cassava: bacteria introduction methods, type of Agrobacterium strains, time post-inoculation, introduction of gene VirG and GUS and their expression into different cassava varieties. The results show that GUS expression is more efficient between 5-7 days post-inoculation with the GV3101 strain, moreover the virulence of this strain increases with the VirG gene. Finally, we observed a range of GUS expression pattern between different cassava varieties, altogether showing that the genetic factor is important for an accurate and effective agroinfiltration in cassava.

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Main Authors: Ramírez, Edilene, Szurek, Boris, Lopez Carrascal, Camilo Ernesto
Format: Digital revista
Language:spa
Published: Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología 2018
Online Access:https://revistas.unal.edu.co/index.php/biotecnologia/article/view/77063
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institution UNAL
collection OJS
country Colombia
countrycode CO
component Revista
access En linea
databasecode rev-biotecnologia-co
tag revista
region America del Sur
libraryname Sistema Nacional de Bibliotecas de la UNAL
language spa
format Digital
author Ramírez, Edilene
Szurek, Boris
Lopez Carrascal, Camilo Ernesto
spellingShingle Ramírez, Edilene
Szurek, Boris
Lopez Carrascal, Camilo Ernesto
Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz)
author_facet Ramírez, Edilene
Szurek, Boris
Lopez Carrascal, Camilo Ernesto
author_sort Ramírez, Edilene
title Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz)
title_short Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz)
title_full Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz)
title_fullStr Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz)
title_full_unstemmed Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz)
title_sort factors affecting the transient expression of gene gus in cassava (manihot esculenta crantz)
description Transient expression is a common technique used for the co-expression of proteins for a wide range of experiments in molecular biology. This technique has been frequently used for gene study in plant pathogen interaction and has proved to be versatile and effective. However, there are still few reports and sparse data for transient expression in cassava leaves from adult/mature plants. For this reason, in this study we evaluated some regards of transient expression for the gene GUS in cassava: bacteria introduction methods, type of Agrobacterium strains, time post-inoculation, introduction of gene VirG and GUS and their expression into different cassava varieties. The results show that GUS expression is more efficient between 5-7 days post-inoculation with the GV3101 strain, moreover the virulence of this strain increases with the VirG gene. Finally, we observed a range of GUS expression pattern between different cassava varieties, altogether showing that the genetic factor is important for an accurate and effective agroinfiltration in cassava.
publisher Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología
publishDate 2018
url https://revistas.unal.edu.co/index.php/biotecnologia/article/view/77063
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spelling oai:www.revistas.unal.edu.co:article-770632019-02-14T20:29:57Z Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz) Factores que afectan la expresión transitoria del gen GUS en yuca (Manihot esculenta Crantz) Fatores que afetam a expressão transitória do gene GUS na mandioca (Manihot esculenta Crantz) Ramírez, Edilene Szurek, Boris Lopez Carrascal, Camilo Ernesto Agrobacterium tumefaciens beta-glucuronidase VirG agroinfiltration cassava Agrobacterium tumefaciens Beta-glucuronidasa VirG agroinfiltración yuca Agrobacterium tumefaciens Beta-glucuronidase VirG agroinfiltração mandioca Transient expression is a common technique used for the co-expression of proteins for a wide range of experiments in molecular biology. This technique has been frequently used for gene study in plant pathogen interaction and has proved to be versatile and effective. However, there are still few reports and sparse data for transient expression in cassava leaves from adult/mature plants. For this reason, in this study we evaluated some regards of transient expression for the gene GUS in cassava: bacteria introduction methods, type of Agrobacterium strains, time post-inoculation, introduction of gene VirG and GUS and their expression into different cassava varieties. The results show that GUS expression is more efficient between 5-7 days post-inoculation with the GV3101 strain, moreover the virulence of this strain increases with the VirG gene. Finally, we observed a range of GUS expression pattern between different cassava varieties, altogether showing that the genetic factor is important for an accurate and effective agroinfiltration in cassava. La expresión transitoria es una métodología ampliamente utilizada para el estudio de genes. Sin embargo, hasta la fecha no existe un reporte en donde se utilice esta técnica en hojas de yuca de plantas adultas. Por esta razón este trabajo se centró en la determinación de algunos parámetros críticos para la expresión transitoria del gen GUS en yuca como son: la metodología para introducir la bacteria, la cepa de Agrobacterium, el tiempo post-inoculación, la introducción del gen VirG y la expresión del gen GUS en algunas variedades de yuca. Los resultados indicaron niveles más altos de expresión del gen GUS entre 5-7 días post-inoculación (dpi), agroinfiltrando con la cepa GV3101 y un incremento en la virulencia de esta cepa mediante la introducción del gen VirG. Por último se observaron diferentes niveles de expresión del gen GUS entre las variedades de yuca evaluadas, lo que indica que el factor genético es clave en la eficiencia de la agroinfiltración en este cultivo. A expressão transitória é uma metodologia amplamente utilizada para o estudo de genes. Entretanto, até o momento não há relato onde esta técnica é usada em folhas de mandioca de plantas adultas. Por esta razão, este trabalho focou-se na identificação de alguns parâmetros críticos para a expressão transiente do gene GUS em mandioca como são os métodos para a introdução da bactéria Agrobacterium estirpe, o tempo de pós-inoculação, a introdução do gene virG e a expressão do gene GUS em algumas variedades de mandioca. Os resultados indicam níveis mais elevados de expressão do gene GUS entre pós-inoculação (dpi), com agroinfiltrando GV3101 estirpe e aumentou de virulência desta estirpe por introdução do gene VirG 5-7 dias. Finalmente, diferentes níveis de expressão do gene GUS foram observados entre as variedades de mandioca avaliadas, o que indica que o fator genético é fundamental na eficiência da agroinfiltração nessa cultura. Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología 2018-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf https://revistas.unal.edu.co/index.php/biotecnologia/article/view/77063 10.15446/rev.colomb.biote.v20n2.77063 Revista Colombiana de Biotecnología; Vol. 20 No. 2 (2018); 57-67 Revista Colombiana de Biotecnología; Vol. 20 Núm. 2 (2018); 57-67 1909-8758 0123-3475 spa https://revistas.unal.edu.co/index.php/biotecnologia/article/view/77063/doc https://revistas.unal.edu.co/index.php/biotecnologia/article/view/77063/pdf Derechos de autor 2018 Revista Colombiana de Biotecnología https://creativecommons.org/licenses/by/4.0

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