Molecular identification of Potyviruses infecting potato plots in Eastern Antioquia (Colombia)
Potyviruses are one of the most limiting viruses for the potato (Solanum tuberosum and S. phureja) production worldwide, being PVY, PVV and PVA the most prevalent species. In this work, we tested the presence of these viruses in four commercial S. tuberosum cv. Diacol-Capiro and S. phureja cv. Criolla-Colombia plots in eastern Antioquia using RT-PCR and Sanger sequencing of the coat protein (CP) region, as well as next generation sequencing (NGS) for S. tuberosum samples. Our results revealed the presence of a PVV strain infecting S. phureja with high sequence identity (>99%) to lineage PVVPhu previously reported in Antioquia. Three strains of PVY (PVYN, PVYNTN and PVYO) were found to infect S. tuberosum. The presence of these three PVY strains was confirmed by NGS, allowing the assembly of their complete genomes. This is the first report of the full genome sequence of PVYo strain in Colombia. These findings highlight the need to adjust the tuber-seed certification programs currently implemented in the country.
| Auteurs principaux: | , , |
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| Format: | Digital revista |
| Langue: | spa |
| Publié: |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología
2018
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| Accès en ligne: | https://revistas.unal.edu.co/index.php/actabiol/article/view/65683 |
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