Virus diagnosis in tree tomato (Solanum betaceum Cav.) by RT-PCR and transmission electrón microscopy in Pichincha and Tungurahua Provinces of Ecuador
Tree tomato (Solanum betaceum Cav.) is an economically important fruit crop in Ecuador. The symptoms of higher virus-related incidence in plantations located in Pichincha and Tungurahua correspond to yellowing, mosaic, leaf deformation and blistering. This research carried out a diagnosis of the viruses present in these production areas using molecular techniques (RT-PCR) and electron microscopy. The identified viruses belonged to the genera Potyvirus (Potato virus Y; PVY), Polerovirus (Potato leaf roll virus; PLRV) and Tobamovirus (Tomato mosaic virus;ToMV), constituting a viral complex of at least two of the three viruses found. The symptomatology related to PVY was spots with tan or oily appearance and leaf deformation; in addition to these symptoms, yellowing and chlorosis were related to PLRV. Foliar blistering was related to the occurrence of the three viruses. The results indicated the presence of the three viruses in Tumbaco (Pichincha) and two (PLRV and PVY) in Pelileo (Tungurahua). PLRV was found in the 100% of the total samples, PVY in 50% of the Pelileo's samples and ToMV only in Tumbaco. ToMV virus has been detected for the first time infecting tree tomato in Ecuador.
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Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrarias
2017
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oai:www.revistas.unal.edu.co:article-609602020-12-20T22:23:16Z Virus diagnosis in tree tomato (Solanum betaceum Cav.) by RT-PCR and transmission electrón microscopy in Pichincha and Tungurahua Provinces of Ecuador Diagnosis de virus en tomate de árbol (Solanum betaceum Cav.) mediante RT-PCR y microscopía electrónica de transmisión en Pichincha y Tungurahua Provincias de Ecuador Espinoza, Diego Viera, William Debut, Alexis Vásquez, Wilson Ayala, Ligia Potyvirus ToMV leaf deformation chlorosis Potyvirus ToMV deformación foliar clorosis virología Tree tomato (Solanum betaceum Cav.) is an economically important fruit crop in Ecuador. The symptoms of higher virus-related incidence in plantations located in Pichincha and Tungurahua correspond to yellowing, mosaic, leaf deformation and blistering. This research carried out a diagnosis of the viruses present in these production areas using molecular techniques (RT-PCR) and electron microscopy. The identified viruses belonged to the genera Potyvirus (Potato virus Y; PVY), Polerovirus (Potato leaf roll virus; PLRV) and Tobamovirus (Tomato mosaic virus;ToMV), constituting a viral complex of at least two of the three viruses found. The symptomatology related to PVY was spots with tan or oily appearance and leaf deformation; in addition to these symptoms, yellowing and chlorosis were related to PLRV. Foliar blistering was related to the occurrence of the three viruses. The results indicated the presence of the three viruses in Tumbaco (Pichincha) and two (PLRV and PVY) in Pelileo (Tungurahua). PLRV was found in the 100% of the total samples, PVY in 50% of the Pelileo's samples and ToMV only in Tumbaco. ToMV virus has been detected for the first time infecting tree tomato in Ecuador. Tomate de árbol (Solanum betaceum Cav.) es un frutal de importancia económica en Ecuador. La sintomatología relacionada con alta incidencia de virus en plantaciones localizadas en Pichincha y Tungurahua corresponde a amarillamiento, mosaicos, deformación foliar y ampollamiento. Este estudio realizó un diagnóstico de virus presentes en estas zonas de producción utilizando técnicas moleculares (RT-PCR) y microscopía electrónica). Los virus identificados correspondieron a losgéneros Potyvirus (Virus Y de la papa; PVY), Polerovirus (Virus del enrollamiento de la papa; PLRV) and Tobamovirus (Virus del mosaico del tomate; ToMV), constituyendo un complejo viral de al menos dos de los tres virus identificados. La sintomatología relacionada a la presencia de PVY fue manchas de apariencia aceitosa y deformación de la hoja; adicionalmente a estos síntomas, amarillamiento y clorosis estuvo relacionado con PLRV. Ampollamiento de las hojas estuvo relacionado con la existencia de los tres virus. Los resultados indicaron la presencia de los tres virus en Tumbaco (Pichincha) y dos (PLRV y PVY) en Pelileo (Tungurahua). PLRV fue encontrado en el 100% del total de muestras analizadas, PVY en 50% de las muestras en Pelileo y ToMV solo en Tumbaco. Se ha detectado por primera vez el virus ToMV infectando tomate de árbol en Ecuador. Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrarias 2017-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion experimental application/pdf https://revistas.unal.edu.co/index.php/agrocol/article/view/60960 10.15446/agron.colomb.v35n1.60960 Agronomía Colombiana; Vol. 35 No. 1 (2017); 35-43 Agronomía Colombiana; Vol. 35 Núm. 1 (2017); 35-43 Agronomía Colombiana; v. 35 n. 1 (2017); 35-43 2357-3732 0120-9965 eng https://revistas.unal.edu.co/index.php/agrocol/article/view/60960/60394 Copyright (c) 2017 Agronomía Colombiana https://creativecommons.org/licenses/by-nc-sa/4.0 |
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| author |
Espinoza, Diego Viera, William Debut, Alexis Vásquez, Wilson Ayala, Ligia |
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Espinoza, Diego Viera, William Debut, Alexis Vásquez, Wilson Ayala, Ligia Virus diagnosis in tree tomato (Solanum betaceum Cav.) by RT-PCR and transmission electrón microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| author_facet |
Espinoza, Diego Viera, William Debut, Alexis Vásquez, Wilson Ayala, Ligia |
| author_sort |
Espinoza, Diego |
| title |
Virus diagnosis in tree tomato (Solanum betaceum Cav.) by RT-PCR and transmission electrón microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| title_short |
Virus diagnosis in tree tomato (Solanum betaceum Cav.) by RT-PCR and transmission electrón microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| title_full |
Virus diagnosis in tree tomato (Solanum betaceum Cav.) by RT-PCR and transmission electrón microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| title_fullStr |
Virus diagnosis in tree tomato (Solanum betaceum Cav.) by RT-PCR and transmission electrón microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| title_full_unstemmed |
Virus diagnosis in tree tomato (Solanum betaceum Cav.) by RT-PCR and transmission electrón microscopy in Pichincha and Tungurahua Provinces of Ecuador |
| title_sort |
virus diagnosis in tree tomato (solanum betaceum cav.) by rt-pcr and transmission electrón microscopy in pichincha and tungurahua provinces of ecuador |
| description |
Tree tomato (Solanum betaceum Cav.) is an economically important fruit crop in Ecuador. The symptoms of higher virus-related incidence in plantations located in Pichincha and Tungurahua correspond to yellowing, mosaic, leaf deformation and blistering. This research carried out a diagnosis of the viruses present in these production areas using molecular techniques (RT-PCR) and electron microscopy. The identified viruses belonged to the genera Potyvirus (Potato virus Y; PVY), Polerovirus (Potato leaf roll virus; PLRV) and Tobamovirus (Tomato mosaic virus;ToMV), constituting a viral complex of at least two of the three viruses found. The symptomatology related to PVY was spots with tan or oily appearance and leaf deformation; in addition to these symptoms, yellowing and chlorosis were related to PLRV. Foliar blistering was related to the occurrence of the three viruses. The results indicated the presence of the three viruses in Tumbaco (Pichincha) and two (PLRV and PVY) in Pelileo (Tungurahua). PLRV was found in the 100% of the total samples, PVY in 50% of the Pelileo's samples and ToMV only in Tumbaco. ToMV virus has been detected for the first time infecting tree tomato in Ecuador. |
| publisher |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias Agrarias |
| publishDate |
2017 |
| url |
https://revistas.unal.edu.co/index.php/agrocol/article/view/60960 |
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