Genetic Diversity in a Population of Rhinoclemmys nasuta (Testudines:Geoemydidae) Associatted with an Insular Locality in the Chocó Biogeographic Region

Genetic Diversity in a Population of Rhinoclemmys nasuta (Testudines:Geoemydidae) Associatted with an Insular Locality in the Chocó Biogeographic Region

Characterization of the genetic diversity of Rhinoclemmys nasuta population inhabits Isla Palma (Malaga Bay, Valle del Cauca) was carried out using three microsatellite systems (Cm72 , Cm58 and Cm3). In this locality, individuals of R. nasuta are widely distributed in the inland streams and creeks system. 100 to 200 mL of peripheral blood was taken off from ten turtles in five streams of the island, preserving samples in a 0.5 M EDTA. DNA was extracted using Salting-out and Chelex solution techniques. PCR amplified products were visualized and measured in polyacrylamide gels stained with silver nitrate. Successful amplification products were obtained for all systems analyzed, two of which (Cm72 and Cm3) were found to be monomorphic, while the system Cm58 had a high PIC (0.698) allowing to estimate the genetic diversity of this population. The observed heterozygosity was low (Ho = 0.26) and inbreeding indices FIS and FIT were high (0.67857 and 0.67881), indicating an excess of homozygotes in each of the rivers and for the all population. The molecular analysis of variance suggested that there is no difference in genetic structure of the population (FST = 0.00075, p= 0.95112). Therefore, the results suggest that the genetic diversity of R. nasuta population in Isla Palma was low and exhibited a highly inbred index.

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Main Authors: Castillo Cutiva, Leslie Anais, Barreto, Guillermo, Giraldo, Alan
Format: Digital revista
Language:spa
Published: Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología 2014
Online Access:https://revistas.unal.edu.co/index.php/actabiol/article/view/42776
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access En linea
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language spa
format Digital
author Castillo Cutiva, Leslie Anais
Barreto, Guillermo
Giraldo, Alan
spellingShingle Castillo Cutiva, Leslie Anais
Barreto, Guillermo
Giraldo, Alan
Genetic Diversity in a Population of Rhinoclemmys nasuta (Testudines:Geoemydidae) Associatted with an Insular Locality in the Chocó Biogeographic Region
author_facet Castillo Cutiva, Leslie Anais
Barreto, Guillermo
Giraldo, Alan
author_sort Castillo Cutiva, Leslie Anais
title Genetic Diversity in a Population of Rhinoclemmys nasuta (Testudines:Geoemydidae) Associatted with an Insular Locality in the Chocó Biogeographic Region
title_short Genetic Diversity in a Population of Rhinoclemmys nasuta (Testudines:Geoemydidae) Associatted with an Insular Locality in the Chocó Biogeographic Region
title_full Genetic Diversity in a Population of Rhinoclemmys nasuta (Testudines:Geoemydidae) Associatted with an Insular Locality in the Chocó Biogeographic Region
title_fullStr Genetic Diversity in a Population of Rhinoclemmys nasuta (Testudines:Geoemydidae) Associatted with an Insular Locality in the Chocó Biogeographic Region
title_full_unstemmed Genetic Diversity in a Population of Rhinoclemmys nasuta (Testudines:Geoemydidae) Associatted with an Insular Locality in the Chocó Biogeographic Region
title_sort genetic diversity in a population of rhinoclemmys nasuta (testudines:geoemydidae) associatted with an insular locality in the chocó biogeographic region
description Characterization of the genetic diversity of Rhinoclemmys nasuta population inhabits Isla Palma (Malaga Bay, Valle del Cauca) was carried out using three microsatellite systems (Cm72 , Cm58 and Cm3). In this locality, individuals of R. nasuta are widely distributed in the inland streams and creeks system. 100 to 200 mL of peripheral blood was taken off from ten turtles in five streams of the island, preserving samples in a 0.5 M EDTA. DNA was extracted using Salting-out and Chelex solution techniques. PCR amplified products were visualized and measured in polyacrylamide gels stained with silver nitrate. Successful amplification products were obtained for all systems analyzed, two of which (Cm72 and Cm3) were found to be monomorphic, while the system Cm58 had a high PIC (0.698) allowing to estimate the genetic diversity of this population. The observed heterozygosity was low (Ho = 0.26) and inbreeding indices FIS and FIT were high (0.67857 and 0.67881), indicating an excess of homozygotes in each of the rivers and for the all population. The molecular analysis of variance suggested that there is no difference in genetic structure of the population (FST = 0.00075, p= 0.95112). Therefore, the results suggest that the genetic diversity of R. nasuta population in Isla Palma was low and exhibited a highly inbred index.
publisher Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología
publishDate 2014
url https://revistas.unal.edu.co/index.php/actabiol/article/view/42776
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spelling oai:www.revistas.unal.edu.co:article-427762018-02-13T14:16:55Z Genetic Diversity in a Population of Rhinoclemmys nasuta (Testudines:Geoemydidae) Associatted with an Insular Locality in the Chocó Biogeographic Region DIVERSIDAD GENÉTICA EN UNA POBLACION DE Rhinoclemmys nasuta (TESTUDINES:GEOEMYDIDAE) ASOCIADA AUN AMBIENTE INSULAR DEL CHOCÓ BIOGEOGRÁFICO Castillo Cutiva, Leslie Anais Barreto, Guillermo Giraldo, Alan Chocó Colombia conservation large-nosed wood turtle Neotropical turtles turtles. Chocó Biogeográfico Colombia conservación Neotrópico tortugas tortuga río Chocoana. Characterization of the genetic diversity of Rhinoclemmys nasuta population inhabits Isla Palma (Malaga Bay, Valle del Cauca) was carried out using three microsatellite systems (Cm72 , Cm58 and Cm3). In this locality, individuals of R. nasuta are widely distributed in the inland streams and creeks system. 100 to 200 mL of peripheral blood was taken off from ten turtles in five streams of the island, preserving samples in a 0.5 M EDTA. DNA was extracted using Salting-out and Chelex solution techniques. PCR amplified products were visualized and measured in polyacrylamide gels stained with silver nitrate. Successful amplification products were obtained for all systems analyzed, two of which (Cm72 and Cm3) were found to be monomorphic, while the system Cm58 had a high PIC (0.698) allowing to estimate the genetic diversity of this population. The observed heterozygosity was low (Ho = 0.26) and inbreeding indices FIS and FIT were high (0.67857 and 0.67881), indicating an excess of homozygotes in each of the rivers and for the all population. The molecular analysis of variance suggested that there is no difference in genetic structure of the population (FST = 0.00075, p= 0.95112). Therefore, the results suggest that the genetic diversity of R. nasuta population in Isla Palma was low and exhibited a highly inbred index. Se realizó la caracterización de la diversidad genética de la población de Rhinoclemmys nasuta, que habita en la localidad insular de Isla Palma, Bahía Málaga (Valle del Cauca), utilizando tres sistemas de microsatélites (Cm72, Cm58 y Cm3). En esta localidad, R. nasuta se encuentra ampliamente distribuida en los sistemas de riachuelos y quebradas presentes. Se tomaron entre 100 a 200 µL de sangre periférica de diez tortugas en cinco riachuelos, preservando las muestras en una solución 0,5 M de EDTA y el ADN fue extraído mediante las técnicas de Salting-out y Chelex. Los productos amplificados por PCR fueron visualizados y medidos en geles de poliacrilamida teñidos con nitrato de plata. Se obtuvieron productos amplificados exitosos para todos los sistemas analizados, dos de los cuales (Cm72 y Cm3) resultaron ser monomórficos, mientras que el sistema Cm58 presentó un PIC alto (0,698) permitiendo estimar la diversidad genética de esta población. La heterocigosidad observada fue baja (Ho = 0,26) y los índices de endogamia FIS y FIT fueron altos (0,67857 y 0,67881), indicando un exceso de homocigotos en cada uno de los ríos y para la población total. El Análisis Molecular de Varianza sugirió que no existe estructura genética diferencial en esta población (FST = 0,00075; p = 0,95112). En conclusión los resultados sugieren que la diversidad genética de la población de R. nasuta en Isla Palma es baja, siendo una población altamente endogámica  Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología 2014-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion "Artículo revisado por pares" application/pdf text/html https://revistas.unal.edu.co/index.php/actabiol/article/view/42776 10.15446/abc.v19n3.42776 Acta Biológica Colombiana; Vol. 19 No. 3 (2014): Tortugas de Colombia; 513-519 Acta Biológica Colombiana; Vol. 19 Núm. 3 (2014): Tortugas de Colombia; 513-519 1900-1649 0120-548X spa https://revistas.unal.edu.co/index.php/actabiol/article/view/42776/46303 https://revistas.unal.edu.co/index.php/actabiol/article/view/42776/46920 Derechos de autor 2014 Acta Biológica Colombiana https://creativecommons.org/licenses/by/4.0

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