Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a
In four mountain aquatic ecosystems of altiplano Cundiboyacense, Colombia, we compared through analysis regression data set of chlorophyll-a obtained with spectoropho-tometric (tricromatic formula) and fluorometric (Welschmeyer) methods. The analysis showed that the spectrophotometric method overestimated the chlorophyll-a concen-tration, but it could be used with precaution in the lower trophic environments. An equation that relates the two chlorophyll-a measures was obteined.
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Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología
2005
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oai:www.revistas.unal.edu.co:article-271342012-02-01T20:54:35Z Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a Comparación de la estimación de la Clorofila-a mediante los métodos espectrofotométrico y fluorométrico Rivera R., Carlos Zapata A., Ángela Pinilla, Gabriel Donato, Jhon Chaparro, Beatriz Jiménez, Paola Chlorophyll-A Spectrophotometry Fluorometry Wetlands Mountain Aquatic Ecosystems Clorofila-A Espectrofotometría Fluorometría Humedales Ecosistemas Acuáticos De Montaña In four mountain aquatic ecosystems of altiplano Cundiboyacense, Colombia, we compared through analysis regression data set of chlorophyll-a obtained with spectoropho-tometric (tricromatic formula) and fluorometric (Welschmeyer) methods. The analysis showed that the spectrophotometric method overestimated the chlorophyll-a concen-tration, but it could be used with precaution in the lower trophic environments. An equation that relates the two chlorophyll-a measures was obteined. En cuatro ecosistemas acuáticos de montaña del altiplano Cundiboyacense, Colombia, se compararon mediante un análisis de regresión los datos de clorofila-a obtenidos a partir de los métodos espectrofotométrico (fórmula tricromática) y fluorométrico(método de Welschmeyer). El análisis demostró que el método espectrofotométrico sobreestimó la concentración de clorofila-a, pero se puede utilizar con precaución en ambientes de baja trofia. Se halló una ecuación que permite relacionar las medidas de clorofila-a obtenidas con las dos metodologías. Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología 2005-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unal.edu.co/index.php/actabiol/article/view/27134 Acta Biológica Colombiana; Vol. 10 No. 2 (2005); 95-103 Acta Biológica Colombiana; Vol. 10 Núm. 2 (2005); 95-103 1900-1649 0120-548X spa https://revistas.unal.edu.co/index.php/actabiol/article/view/27134/27409 Derechos de autor 2005 Acta Biológica Colombiana https://creativecommons.org/licenses/by/4.0 |
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Colombia |
| countrycode |
CO |
| component |
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| tag |
revista |
| region |
America del Sur |
| libraryname |
Sistema Nacional de Bibliotecas de la UNAL |
| language |
spa |
| format |
Digital |
| author |
Rivera R., Carlos Zapata A., Ángela Pinilla, Gabriel Donato, Jhon Chaparro, Beatriz Jiménez, Paola |
| spellingShingle |
Rivera R., Carlos Zapata A., Ángela Pinilla, Gabriel Donato, Jhon Chaparro, Beatriz Jiménez, Paola Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a |
| author_facet |
Rivera R., Carlos Zapata A., Ángela Pinilla, Gabriel Donato, Jhon Chaparro, Beatriz Jiménez, Paola |
| author_sort |
Rivera R., Carlos |
| title |
Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a |
| title_short |
Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a |
| title_full |
Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a |
| title_fullStr |
Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a |
| title_full_unstemmed |
Comparation of Spectrophotometric and Fluorometric Measurements of Chlorophyll-a |
| title_sort |
comparation of spectrophotometric and fluorometric measurements of chlorophyll-a |
| description |
In four mountain aquatic ecosystems of altiplano Cundiboyacense, Colombia, we compared through analysis regression data set of chlorophyll-a obtained with spectoropho-tometric (tricromatic formula) and fluorometric (Welschmeyer) methods. The analysis showed that the spectrophotometric method overestimated the chlorophyll-a concen-tration, but it could be used with precaution in the lower trophic environments. An equation that relates the two chlorophyll-a measures was obteined. |
| publisher |
Universidad Nacional de Colombia - Sede Bogotá - Facultad de Ciencias - Departamento de Biología |
| publishDate |
2005 |
| url |
https://revistas.unal.edu.co/index.php/actabiol/article/view/27134 |
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