Identificación de metodologías para la evaluación de tolerancia a temperaturas bajas en arroz (Oryza sativa L.)
In this study the methodologies for the evaluation of the cold tolerance level of rice in the following stages were identified: germination from sterilized dry seeds (placed on a Petri dish at 13°C up the time when the controls showed 70 % of the seeds with coleoptile 5 mm or 35 days after planting), pregerminated seeds (incubated 5° C for 15 days and planted on sterilized soil and kept at ambient for 7 days) and in the seedling stage (seedlings with 3rd leaf well developed were kept at 13°C for 7 days, transferred to ambient temperature for other 7 days). This methodologies, utilizing non-sophisticated equipment, were capable of evaluating large quantities of genotypes in a short time. With this methodologies were evaluated in F s and R2 (double haploids from the anther culture) 624lines which coming from crosses destined 10 improve the cooking quality of genotypes from temperate zones and 19 lines were identified from the evaluation that combined tolerance to low temperature in the three evaluated stages and excellent cooking quality (larges or extralarges grain sizes, low white belly and more of 23 % amylose content).
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Universidad Nacional de Colombia - Sede Palmira
1989
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oai:www.revistas.unal.edu.co:article-153802010-09-17T16:43:28Z Identificación de metodologías para la evaluación de tolerancia a temperaturas bajas en arroz (Oryza sativa L.) Mejía M., Olga Isabel Pulver, Edward L. bajas temperaturas arroz semilla seca esterilizada plántula granos largos extralargos amilosa Oryza sativa L. cold tolerance rice dry seeds In this study the methodologies for the evaluation of the cold tolerance level of rice in the following stages were identified: germination from sterilized dry seeds (placed on a Petri dish at 13°C up the time when the controls showed 70 % of the seeds with coleoptile 5 mm or 35 days after planting), pregerminated seeds (incubated 5° C for 15 days and planted on sterilized soil and kept at ambient for 7 days) and in the seedling stage (seedlings with 3rd leaf well developed were kept at 13°C for 7 days, transferred to ambient temperature for other 7 days). This methodologies, utilizing non-sophisticated equipment, were capable of evaluating large quantities of genotypes in a short time. With this methodologies were evaluated in F s and R2 (double haploids from the anther culture) 624lines which coming from crosses destined 10 improve the cooking quality of genotypes from temperate zones and 19 lines were identified from the evaluation that combined tolerance to low temperature in the three evaluated stages and excellent cooking quality (larges or extralarges grain sizes, low white belly and more of 23 % amylose content). En el presente trabajo se identificaron metodologías para evaluar tolerancia a temperaturas bajas del arroz en las etapas de semilla seca esterilizada (se colocaron 50 semillas en una caja de Petri y se sometieron a 13°C hasta que los testigos tolerantes presentaran el 70 % de las semillas con coleoptilo 5 mm o 35 días después de la siembra), semilla pregerminada (se incubaron a 5°C durante 15 días, se sembraron en suelo esterilizado y se mantuvieron a temperatura ambiente por 7 días) y estado de plántula (plántulas con tercera hoja desarrollada se sometieron a 13°C durante 7 días y se transfirieron a temperatura ambiente otros 7 días). Estas metodologías sencillas, utilizando equipos no sofisticados, fueron capaces de evaluar gran cantidad de genotipos y en corto tiempo. Entre 624 líneas en F5 Y R2 (dobles haploides del cultivo de anteras), provenientes de cruces destinados a mejorar la calidad culinaria de genotipos de una zona templada, se identificaron 19 líneas que combinaron la tolerancia a temperaturas bajas en los tres estados evaluados y excelente calidad culinaria, expresada en granos largos o extralargos bajo centro blanco y contenido de amilosa mayor de 23 %. Universidad Nacional de Colombia - Sede Palmira 1989-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/15380 Acta Agronómica; Vol. 39 No. 1-2 (1989); 7-17 Acta Agronómica; Vol. 39 Núm. 1-2 (1989); 7-17 Acta Agronómica; v. 39 n. 1-2 (1989); 7-17 2323-0118 0120-2812 spa https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/15380/16164 Derechos de autor 1989 Acta Agronómica https://creativecommons.org/licenses/by-nc-nd/4.0 |
| institution |
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| collection |
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| country |
Colombia |
| countrycode |
CO |
| component |
Revista |
| access |
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| databasecode |
rev-actaagronomica |
| tag |
revista |
| region |
America del Sur |
| libraryname |
Sistema Nacional de Bibliotecas de la UNAL |
| language |
spa |
| format |
Digital |
| author |
Mejía M., Olga Isabel Pulver, Edward L. |
| spellingShingle |
Mejía M., Olga Isabel Pulver, Edward L. Identificación de metodologías para la evaluación de tolerancia a temperaturas bajas en arroz (Oryza sativa L.) |
| author_facet |
Mejía M., Olga Isabel Pulver, Edward L. |
| author_sort |
Mejía M., Olga Isabel |
| title |
Identificación de metodologías para la evaluación de tolerancia a temperaturas bajas en arroz (Oryza sativa L.) |
| title_short |
Identificación de metodologías para la evaluación de tolerancia a temperaturas bajas en arroz (Oryza sativa L.) |
| title_full |
Identificación de metodologías para la evaluación de tolerancia a temperaturas bajas en arroz (Oryza sativa L.) |
| title_fullStr |
Identificación de metodologías para la evaluación de tolerancia a temperaturas bajas en arroz (Oryza sativa L.) |
| title_full_unstemmed |
Identificación de metodologías para la evaluación de tolerancia a temperaturas bajas en arroz (Oryza sativa L.) |
| title_sort |
identificación de metodologías para la evaluación de tolerancia a temperaturas bajas en arroz (oryza sativa l.) |
| description |
In this study the methodologies for the evaluation of the cold tolerance level of rice in the following stages were identified: germination from sterilized dry seeds (placed on a Petri dish at 13°C up the time when the controls showed 70 % of the seeds with coleoptile 5 mm or 35 days after planting), pregerminated seeds (incubated 5° C for 15 days and planted on sterilized soil and kept at ambient for 7 days) and in the seedling stage (seedlings with 3rd leaf well developed were kept at 13°C for 7 days, transferred to ambient temperature for other 7 days). This methodologies, utilizing non-sophisticated equipment, were capable of evaluating large quantities of genotypes in a short time. With this methodologies were evaluated in F s and R2 (double haploids from the anther culture) 624lines which coming from crosses destined 10 improve the cooking quality of genotypes from temperate zones and 19 lines were identified from the evaluation that combined tolerance to low temperature in the three evaluated stages and excellent cooking quality (larges or extralarges grain sizes, low white belly and more of 23 % amylose content). |
| publisher |
Universidad Nacional de Colombia - Sede Palmira |
| publishDate |
1989 |
| url |
https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/15380 |
| work_keys_str_mv |
AT mejiamolgaisabel identificaciondemetodologiasparalaevaluaciondetoleranciaatemperaturasbajasenarrozoryzasatival AT pulveredwardl identificaciondemetodologiasparalaevaluaciondetoleranciaatemperaturasbajasenarrozoryzasatival |
| _version_ |
1755913684992917504 |