Lack of evidence for Mycoplasma spp. in bulk tank milk of herds located in mid-western Colombia
Mycoplasma spp. is reported as a highly contagious mastitis-causing bacteria in dairy cattle, without successful or low response to most common antibiotic treatments due to the lack of cell wall. In Colombia it has been reported in the Central Andean region during 2014. The aim was to estimate the prevalence of Mycoplasma spp. in bulk tank milk using microbiological and molecular diagnosis. A random longitudinal study enrolling 220 commercial dairy farms located in four provinces of the mid-western region of Colombia from four pasteurizer companies was performed. Bulk tank milk samples were collected once monthly for three months period for determining somatic cell count (SCC) and microbiological and molecular diagnosis of Mycoplasma spp. cultures were done without pre-enrichment procedures directly in mycoplasma agar with cefoperazone to inhibit growth of opportunistic microorganisms, plates were incubated under 37° C and atmosphere of 10% CO2 and inspected during a 10d period. Molecular analysis was done by a multiplex PCR using specific primers targeting the 16S-23S rARN gene of Mycoplasma spp. and from non-pathogenic bacteria occasionally found in milk. LnSCC average of included dairy farms was 6.19 x103 cells/mL, Mycoplasma spp. was not isolated during microbiological cultures, and no DNA belonging to the species was detected by PCR in the 220 bulk tanks milk, with an estimated prevalence lower than 2.3%. This finding shows that there is not microbiological or molecular evidence that demonstrates the presence of the pathogen in the milk from the mid-western region of Colombia at herd level.
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Universidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina Veterinaria y de Zootecnia
2022
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Online Access: | https://revistas.unal.edu.co/index.php/remevez/article/view/103807 |
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Velasco-Bolaños, Juan Jaramillo–Jaramillo, A. S. Villa–Arcila, N. A. Dufour, S. Ceballos–Márquez, A. Piepers, S. |
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Velasco-Bolaños, Juan Jaramillo–Jaramillo, A. S. Villa–Arcila, N. A. Dufour, S. Ceballos–Márquez, A. Piepers, S. Lack of evidence for Mycoplasma spp. in bulk tank milk of herds located in mid-western Colombia |
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Velasco-Bolaños, Juan Jaramillo–Jaramillo, A. S. Villa–Arcila, N. A. Dufour, S. Ceballos–Márquez, A. Piepers, S. |
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Velasco-Bolaños, Juan |
title |
Lack of evidence for Mycoplasma spp. in bulk tank milk of herds located in mid-western Colombia |
title_short |
Lack of evidence for Mycoplasma spp. in bulk tank milk of herds located in mid-western Colombia |
title_full |
Lack of evidence for Mycoplasma spp. in bulk tank milk of herds located in mid-western Colombia |
title_fullStr |
Lack of evidence for Mycoplasma spp. in bulk tank milk of herds located in mid-western Colombia |
title_full_unstemmed |
Lack of evidence for Mycoplasma spp. in bulk tank milk of herds located in mid-western Colombia |
title_sort |
lack of evidence for mycoplasma spp. in bulk tank milk of herds located in mid-western colombia |
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Mycoplasma spp. is reported as a highly contagious mastitis-causing bacteria in dairy cattle, without successful or low response to most common antibiotic treatments due to the lack of cell wall. In Colombia it has been reported in the Central Andean region during 2014. The aim was to estimate the prevalence of Mycoplasma spp. in bulk tank milk using microbiological and molecular diagnosis. A random longitudinal study enrolling 220 commercial dairy farms located in four provinces of the mid-western region of Colombia from four pasteurizer companies was performed. Bulk tank milk samples were collected once monthly for three months period for determining somatic cell count (SCC) and microbiological and molecular diagnosis of Mycoplasma spp. cultures were done without pre-enrichment procedures directly in mycoplasma agar with cefoperazone to inhibit growth of opportunistic microorganisms, plates were incubated under 37° C and atmosphere of 10% CO2 and inspected during a 10d period. Molecular analysis was done by a multiplex PCR using specific primers targeting the 16S-23S rARN gene of Mycoplasma spp. and from non-pathogenic bacteria occasionally found in milk. LnSCC average of included dairy farms was 6.19 x103 cells/mL, Mycoplasma spp. was not isolated during microbiological cultures, and no DNA belonging to the species was detected by PCR in the 220 bulk tanks milk, with an estimated prevalence lower than 2.3%. This finding shows that there is not microbiological or molecular evidence that demonstrates the presence of the pathogen in the milk from the mid-western region of Colombia at herd level.
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Universidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina Veterinaria y de Zootecnia |
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2022 |
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https://revistas.unal.edu.co/index.php/remevez/article/view/103807 |
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oai:www.revistas.unal.edu.co:article-1038072022-12-09T18:20:11Z Lack of evidence for Mycoplasma spp. in bulk tank milk of herds located in mid-western Colombia Falta de evidencia para Mycoplasma spp. en leche de tanques de enfriamiento de hatos del centro-occidente colombiano Velasco-Bolaños, Juan Jaramillo–Jaramillo, A. S. Villa–Arcila, N. A. Dufour, S. Ceballos–Márquez, A. Piepers, S. molecular prevalence dairy cow mastitis prevalencia vacas lecheras Mycoplasma spp. is reported as a highly contagious mastitis-causing bacteria in dairy cattle, without successful or low response to most common antibiotic treatments due to the lack of cell wall. In Colombia it has been reported in the Central Andean region during 2014. The aim was to estimate the prevalence of Mycoplasma spp. in bulk tank milk using microbiological and molecular diagnosis. A random longitudinal study enrolling 220 commercial dairy farms located in four provinces of the mid-western region of Colombia from four pasteurizer companies was performed. Bulk tank milk samples were collected once monthly for three months period for determining somatic cell count (SCC) and microbiological and molecular diagnosis of Mycoplasma spp. cultures were done without pre-enrichment procedures directly in mycoplasma agar with cefoperazone to inhibit growth of opportunistic microorganisms, plates were incubated under 37° C and atmosphere of 10% CO2 and inspected during a 10d period. Molecular analysis was done by a multiplex PCR using specific primers targeting the 16S-23S rARN gene of Mycoplasma spp. and from non-pathogenic bacteria occasionally found in milk. LnSCC average of included dairy farms was 6.19 x103 cells/mL, Mycoplasma spp. was not isolated during microbiological cultures, and no DNA belonging to the species was detected by PCR in the 220 bulk tanks milk, with an estimated prevalence lower than 2.3%. This finding shows that there is not microbiological or molecular evidence that demonstrates the presence of the pathogen in the milk from the mid-western region of Colombia at herd level. Mycoplasma spp. es una bacteria causante de mastitis altamente contagiosa en ganado lechero, sin o con baja respuesta a tratamientos antibióticos convencionales debido a que carece de pared celular. En Colombia ha sido reportado en la región Andina Central durante 2014. El objetivo fue estimar la prevalencia de Mycoplasma spp. en leche de tanques de enfriamiento empleando diagnósticos microbiológicos y moleculares. Se realizó un estudio aleatorio longitudinal que incluyó 220 lecherías comerciales en cuatro departamentos del centro–occidente colombiano acopiadas por cuatro compañías pasteurizadoras. Se recolectaron muestras de leche del tanque de enfriamiento mensualmente durante tres meses para determinar el recuento de células somáticas (SCC) y el diagnóstico microbiológico y molecular de Mycoplasma spp. Los cultivos se realizaron sin procedimientos de preenriquecimiento directamente en agar micoplasma con cefoperazona para inhibir crecimiento de microorganismos oportunistas, los agares se incubaron a 37° C con una atmosfera del 10% CO2 e inspeccionados durante 10d. Los análisis moleculares se realizaron por PCR multiplex usando cebadores específicos para los genes 16S-23S rRNA del Mycoplasma spp. y de algunas bacterias oportunistas ocasionales en la leche. El promedio del LnSCC fue de 6.19 x103 células/ mL, Mycoplasma spp. no fue aislado de los cultivos microbiológicos y no se encontró ADN de a esta especie mediante PCR en los 220 tanques de leche. Lo anterior indica una prevalencia estimada menor a 2,3%. Se concluye que no existe evidencia microbiológica ni molecular para demostrar la presencia del patógeno en la leche de la región centro-occidente colombiana a nivel de hato. Universidad Nacional de Colombia - Sede Bogotá - Facultad de Medicina Veterinaria y de Zootecnia 2022-11-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.unal.edu.co/index.php/remevez/article/view/103807 10.15446/rfmvz.v69n3.103807 Revista de la Facultad de Medicina Veterinaria y de Zootecnia; Vol. 69 No. 3 (2022) Revista de la Facultad de Medicina Veterinaria y de Zootecnia; Vol. 69 Núm. 3 (2022) Revista de la Facultad de Medicina Veterinaria y de Zootecnia; v. 69 n. 3 (2022) 2357-3813 0120-2952 eng https://revistas.unal.edu.co/index.php/remevez/article/view/103807/85211 Derechos de autor 2022 Revista de la Facultad de Medicina Veterinaria y de Zootecnia http://creativecommons.org/licenses/by-nc-nd/4.0 |