Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experience

Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experience

ABSTRACT Background: Due to laboratory logistic issues, our center has traditionally scheduled peripheral blood stem cell harvests based on timing from the start of mobilization. This has proved to be useful in some cases, but also resulted in many fruitless harvests due to poor mobilization. In order to improve the efficiency of collections and compare the effectiveness of peripheral blood CD34+ cells as a predictor with data from other reports, this study analyzed the implementation of this routine. Methods: Peripheral blood and leukapheresis samples were quantified by flow cytometry and the association between these parameters was assessed. Results: Sixty-six consecutive leukapheresis samples were collected from 34 patients after the collection of peripheral blood samples for CD34+ quantification. A moderate positive correlation was observed between peripheral blood CD34+ cell count and total CD34+ cell count/kg (r = 0.596; p-value < 0.001). A multivariable regression model also confirmed this association and allowed the estimation that for every increase in five CD34+ cells/µL in the peripheral blood, a mean increase of 0.38 × 106 CD34+ cells/kg could be predicted. Demographic characteristics, baseline comorbidities and mobilization regimen did not influence final CD34+ cell count in this sample. Conclusions: As observed in other centers, quantification of peripheral blood CD34+ progenitor cells is a strong predictor of effectiveness to guide stem cell harvesting. Due to the results of this study, a modification in the peripheral blood stem cell harvesting logistics was implemented at our center in order to incorporate this routine.

Guardado en:
Detalles Bibliográficos
Autores principales: Lemos,Natália Emerim, Farias,Mariela Granero, Kubaski,Francyne, Scotti,Luciana, Onsten,Tor Gunnar Hugo, Brondani,Letícia de Almeida, Wagner,Sandrine Comparsi, Sekine,Leo
Formato: Digital revista
Idioma:English
Publicado: Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular (ABHH) 2018
Acceso en línea:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2531-13792018000300213
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:scielo:S2531-13792018000300213
record_format ojs
spelling oai:scielo:S2531-137920180003002132019-08-06Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experienceLemos,Natália EmerimFarias,Mariela GraneroKubaski,FrancyneScotti,LucianaOnsten,Tor Gunnar HugoBrondani,Letícia de AlmeidaWagner,Sandrine ComparsiSekine,Leo Transplantation Apheresis CD34+ progenitor cells Flow cytometry ABSTRACT Background: Due to laboratory logistic issues, our center has traditionally scheduled peripheral blood stem cell harvests based on timing from the start of mobilization. This has proved to be useful in some cases, but also resulted in many fruitless harvests due to poor mobilization. In order to improve the efficiency of collections and compare the effectiveness of peripheral blood CD34+ cells as a predictor with data from other reports, this study analyzed the implementation of this routine. Methods: Peripheral blood and leukapheresis samples were quantified by flow cytometry and the association between these parameters was assessed. Results: Sixty-six consecutive leukapheresis samples were collected from 34 patients after the collection of peripheral blood samples for CD34+ quantification. A moderate positive correlation was observed between peripheral blood CD34+ cell count and total CD34+ cell count/kg (r = 0.596; p-value < 0.001). A multivariable regression model also confirmed this association and allowed the estimation that for every increase in five CD34+ cells/µL in the peripheral blood, a mean increase of 0.38 × 106 CD34+ cells/kg could be predicted. Demographic characteristics, baseline comorbidities and mobilization regimen did not influence final CD34+ cell count in this sample. Conclusions: As observed in other centers, quantification of peripheral blood CD34+ progenitor cells is a strong predictor of effectiveness to guide stem cell harvesting. Due to the results of this study, a modification in the peripheral blood stem cell harvesting logistics was implemented at our center in order to incorporate this routine.info:eu-repo/semantics/openAccessAssociação Brasileira de Hematologia, Hemoterapia e Terapia Celular (ABHH)Hematology, Transfusion and Cell Therapy v.40 n.3 20182018-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2531-13792018000300213en10.1016/j.htct.2018.01.002
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Lemos,Natália Emerim
Farias,Mariela Granero
Kubaski,Francyne
Scotti,Luciana
Onsten,Tor Gunnar Hugo
Brondani,Letícia de Almeida
Wagner,Sandrine Comparsi
Sekine,Leo
spellingShingle Lemos,Natália Emerim
Farias,Mariela Granero
Kubaski,Francyne
Scotti,Luciana
Onsten,Tor Gunnar Hugo
Brondani,Letícia de Almeida
Wagner,Sandrine Comparsi
Sekine,Leo
Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experience
author_facet Lemos,Natália Emerim
Farias,Mariela Granero
Kubaski,Francyne
Scotti,Luciana
Onsten,Tor Gunnar Hugo
Brondani,Letícia de Almeida
Wagner,Sandrine Comparsi
Sekine,Leo
author_sort Lemos,Natália Emerim
title Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experience
title_short Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experience
title_full Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experience
title_fullStr Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experience
title_full_unstemmed Quantification of peripheral blood CD34+ cells prior to stem cell harvesting by leukapheresis: a single center experience
title_sort quantification of peripheral blood cd34+ cells prior to stem cell harvesting by leukapheresis: a single center experience
description ABSTRACT Background: Due to laboratory logistic issues, our center has traditionally scheduled peripheral blood stem cell harvests based on timing from the start of mobilization. This has proved to be useful in some cases, but also resulted in many fruitless harvests due to poor mobilization. In order to improve the efficiency of collections and compare the effectiveness of peripheral blood CD34+ cells as a predictor with data from other reports, this study analyzed the implementation of this routine. Methods: Peripheral blood and leukapheresis samples were quantified by flow cytometry and the association between these parameters was assessed. Results: Sixty-six consecutive leukapheresis samples were collected from 34 patients after the collection of peripheral blood samples for CD34+ quantification. A moderate positive correlation was observed between peripheral blood CD34+ cell count and total CD34+ cell count/kg (r = 0.596; p-value < 0.001). A multivariable regression model also confirmed this association and allowed the estimation that for every increase in five CD34+ cells/µL in the peripheral blood, a mean increase of 0.38 × 106 CD34+ cells/kg could be predicted. Demographic characteristics, baseline comorbidities and mobilization regimen did not influence final CD34+ cell count in this sample. Conclusions: As observed in other centers, quantification of peripheral blood CD34+ progenitor cells is a strong predictor of effectiveness to guide stem cell harvesting. Due to the results of this study, a modification in the peripheral blood stem cell harvesting logistics was implemented at our center in order to incorporate this routine.
publisher Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular (ABHH)
publishDate 2018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2531-13792018000300213
work_keys_str_mv AT lemosnataliaemerim quantificationofperipheralbloodcd34cellspriortostemcellharvestingbyleukapheresisasinglecenterexperience
AT fariasmarielagranero quantificationofperipheralbloodcd34cellspriortostemcellharvestingbyleukapheresisasinglecenterexperience
AT kubaskifrancyne quantificationofperipheralbloodcd34cellspriortostemcellharvestingbyleukapheresisasinglecenterexperience
AT scottiluciana quantificationofperipheralbloodcd34cellspriortostemcellharvestingbyleukapheresisasinglecenterexperience
AT onstentorgunnarhugo quantificationofperipheralbloodcd34cellspriortostemcellharvestingbyleukapheresisasinglecenterexperience
AT brondanileticiadealmeida quantificationofperipheralbloodcd34cellspriortostemcellharvestingbyleukapheresisasinglecenterexperience
AT wagnersandrinecomparsi quantificationofperipheralbloodcd34cellspriortostemcellharvestingbyleukapheresisasinglecenterexperience
AT sekineleo quantificationofperipheralbloodcd34cellspriortostemcellharvestingbyleukapheresisasinglecenterexperience
_version_ 1756442040679268352

Ejemplares similares