Gene expression during the germination of coffee seed
Abstract: Germination of the coffee (Coffea arabica L.) seed is the result of events that occur simultaneously in the embryo and endosperm. To understand the molecular mechanisms responsible for these events, we undertook a transcriptome analysis of embryo, micropylar and lateral endosperms from 10-day-imbibed seeds. The sequencing yielded contigs coding for 16,813 proteins. From those, 14,005 (~ 83%) were highly similar to at least one protein sequence in the nr database. 162 genes were significantly expressed in the embryo, 36 in the micropylar endosperm and 72 in the lateral endosperm. The tissue specificity analysis of the significantly expressed genes showed that the embryo had the highest proportion of specific genes (113/162, ~70%), while 11 were expressed in the micropylar and lateral endosperms. In the embryo, genes were mainly associated with abiotic stress, cell growth, and intercellular communication. In the micropylar and lateral endosperms, they were associated with abiotic stress and cell wall degradation. The accuracy of RNA-seq data was confirmed by RT-qPCR. This work adds new information about the molecular mechanism involved in coffee seed germination.
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ABRATES - Associação Brasileira de Tecnologia de Sementes
2019
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oai:scielo:S2317-153720190002001682019-06-27Gene expression during the germination of coffee seedSilva,Edvaldo Aparecido Amaral daAcencio,Marcio LuisBovolenta,Luiz AugustoLemke,NeyVarani,Alessandro de MelloBravo,Juliana PereiraHoshino-Bezerra,Andrea AkemiLemos,Eliana Gertrudes Macedo RNA-seq embryo micropylar endosperm lateral endosperm Coffea arabica Abstract: Germination of the coffee (Coffea arabica L.) seed is the result of events that occur simultaneously in the embryo and endosperm. To understand the molecular mechanisms responsible for these events, we undertook a transcriptome analysis of embryo, micropylar and lateral endosperms from 10-day-imbibed seeds. The sequencing yielded contigs coding for 16,813 proteins. From those, 14,005 (~ 83%) were highly similar to at least one protein sequence in the nr database. 162 genes were significantly expressed in the embryo, 36 in the micropylar endosperm and 72 in the lateral endosperm. The tissue specificity analysis of the significantly expressed genes showed that the embryo had the highest proportion of specific genes (113/162, ~70%), while 11 were expressed in the micropylar and lateral endosperms. In the embryo, genes were mainly associated with abiotic stress, cell growth, and intercellular communication. In the micropylar and lateral endosperms, they were associated with abiotic stress and cell wall degradation. The accuracy of RNA-seq data was confirmed by RT-qPCR. This work adds new information about the molecular mechanism involved in coffee seed germination.info:eu-repo/semantics/openAccessABRATES - Associação Brasileira de Tecnologia de SementesJournal of Seed Science v.41 n.2 20192019-04-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372019000200168en10.1590/2317-1545v41n2208263 |
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Silva,Edvaldo Aparecido Amaral da Acencio,Marcio Luis Bovolenta,Luiz Augusto Lemke,Ney Varani,Alessandro de Mello Bravo,Juliana Pereira Hoshino-Bezerra,Andrea Akemi Lemos,Eliana Gertrudes Macedo |
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Silva,Edvaldo Aparecido Amaral da Acencio,Marcio Luis Bovolenta,Luiz Augusto Lemke,Ney Varani,Alessandro de Mello Bravo,Juliana Pereira Hoshino-Bezerra,Andrea Akemi Lemos,Eliana Gertrudes Macedo Gene expression during the germination of coffee seed |
author_facet |
Silva,Edvaldo Aparecido Amaral da Acencio,Marcio Luis Bovolenta,Luiz Augusto Lemke,Ney Varani,Alessandro de Mello Bravo,Juliana Pereira Hoshino-Bezerra,Andrea Akemi Lemos,Eliana Gertrudes Macedo |
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Silva,Edvaldo Aparecido Amaral da |
title |
Gene expression during the germination of coffee seed |
title_short |
Gene expression during the germination of coffee seed |
title_full |
Gene expression during the germination of coffee seed |
title_fullStr |
Gene expression during the germination of coffee seed |
title_full_unstemmed |
Gene expression during the germination of coffee seed |
title_sort |
gene expression during the germination of coffee seed |
description |
Abstract: Germination of the coffee (Coffea arabica L.) seed is the result of events that occur simultaneously in the embryo and endosperm. To understand the molecular mechanisms responsible for these events, we undertook a transcriptome analysis of embryo, micropylar and lateral endosperms from 10-day-imbibed seeds. The sequencing yielded contigs coding for 16,813 proteins. From those, 14,005 (~ 83%) were highly similar to at least one protein sequence in the nr database. 162 genes were significantly expressed in the embryo, 36 in the micropylar endosperm and 72 in the lateral endosperm. The tissue specificity analysis of the significantly expressed genes showed that the embryo had the highest proportion of specific genes (113/162, ~70%), while 11 were expressed in the micropylar and lateral endosperms. In the embryo, genes were mainly associated with abiotic stress, cell growth, and intercellular communication. In the micropylar and lateral endosperms, they were associated with abiotic stress and cell wall degradation. The accuracy of RNA-seq data was confirmed by RT-qPCR. This work adds new information about the molecular mechanism involved in coffee seed germination. |
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ABRATES - Associação Brasileira de Tecnologia de Sementes |
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2019 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372019000200168 |
work_keys_str_mv |
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1756440796060450816 |