The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from Brazil
Abstract Rearing free-range chicken is based on grazing feeding patterns, and these animals could be potential environmental contaminants of Cryptosporidium oocysts for humans and other animals. Therefore, the present study aimed to evaluate the molecular prevalence of Cryptosporidium spp. in free-range chickens from Brazil. A total of 351 fecal samples from chickens were examined from 20 farms. For detection of Cryptosporidium spp., 18S rRNA gene fragments were amplified using a nested PCR reaction. Positive samples were sent for sequencing. The overall prevalence of Cryptosporidium was 25.6% (95% CI = 21.2% - 30.6%). Sequencing of the amplified fragments allowed for the identification of three species: C. meleagridis in 57 (62.6%), C. baileyi in 15 (16.4%), C. parvum in 3 (3.2%) samples, and a new Cryptosporidium genotype (C. genotype BrPR1) in 3 (3.2%) samples. Cryptosporidium genotype BrPR1 has not yet been classified as a species, and its host spectrum is not known. Cryptosporidium, including zoonotic species, exists at a high prevalence in free-range chickens within the region studied.
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Colégio Brasileiro de Parasitologia Veterinária
2017
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oai:scielo:S1984-296120170004004722018-06-19The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from BrazilEwald,Maria Paula de CarvalhoMartins,Felippe Danyel CardosoCaldart,Eloiza TelesVieira,Fernando Emmanuel GonçalvesYamamura,Milton HissashiSasse,João PedroBarros,Luiz Daniel deFreire,Roberta LemosNavarro,Italmar TeodoricoGarcia,João Luis Cryptosporidiosis zoonosis public health epidemiology Abstract Rearing free-range chicken is based on grazing feeding patterns, and these animals could be potential environmental contaminants of Cryptosporidium oocysts for humans and other animals. Therefore, the present study aimed to evaluate the molecular prevalence of Cryptosporidium spp. in free-range chickens from Brazil. A total of 351 fecal samples from chickens were examined from 20 farms. For detection of Cryptosporidium spp., 18S rRNA gene fragments were amplified using a nested PCR reaction. Positive samples were sent for sequencing. The overall prevalence of Cryptosporidium was 25.6% (95% CI = 21.2% - 30.6%). Sequencing of the amplified fragments allowed for the identification of three species: C. meleagridis in 57 (62.6%), C. baileyi in 15 (16.4%), C. parvum in 3 (3.2%) samples, and a new Cryptosporidium genotype (C. genotype BrPR1) in 3 (3.2%) samples. Cryptosporidium genotype BrPR1 has not yet been classified as a species, and its host spectrum is not known. Cryptosporidium, including zoonotic species, exists at a high prevalence in free-range chickens within the region studied.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.26 n.4 20172017-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000400472en10.1590/s1984-29612017068 |
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Ewald,Maria Paula de Carvalho Martins,Felippe Danyel Cardoso Caldart,Eloiza Teles Vieira,Fernando Emmanuel Gonçalves Yamamura,Milton Hissashi Sasse,João Pedro Barros,Luiz Daniel de Freire,Roberta Lemos Navarro,Italmar Teodorico Garcia,João Luis |
| spellingShingle |
Ewald,Maria Paula de Carvalho Martins,Felippe Danyel Cardoso Caldart,Eloiza Teles Vieira,Fernando Emmanuel Gonçalves Yamamura,Milton Hissashi Sasse,João Pedro Barros,Luiz Daniel de Freire,Roberta Lemos Navarro,Italmar Teodorico Garcia,João Luis The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from Brazil |
| author_facet |
Ewald,Maria Paula de Carvalho Martins,Felippe Danyel Cardoso Caldart,Eloiza Teles Vieira,Fernando Emmanuel Gonçalves Yamamura,Milton Hissashi Sasse,João Pedro Barros,Luiz Daniel de Freire,Roberta Lemos Navarro,Italmar Teodorico Garcia,João Luis |
| author_sort |
Ewald,Maria Paula de Carvalho |
| title |
The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from Brazil |
| title_short |
The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from Brazil |
| title_full |
The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from Brazil |
| title_fullStr |
The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from Brazil |
| title_full_unstemmed |
The first study of molecular prevalence and species characterization of Cryptosporidium in free-range chicken (Gallus gallus domesticus) from Brazil |
| title_sort |
first study of molecular prevalence and species characterization of cryptosporidium in free-range chicken (gallus gallus domesticus) from brazil |
| description |
Abstract Rearing free-range chicken is based on grazing feeding patterns, and these animals could be potential environmental contaminants of Cryptosporidium oocysts for humans and other animals. Therefore, the present study aimed to evaluate the molecular prevalence of Cryptosporidium spp. in free-range chickens from Brazil. A total of 351 fecal samples from chickens were examined from 20 farms. For detection of Cryptosporidium spp., 18S rRNA gene fragments were amplified using a nested PCR reaction. Positive samples were sent for sequencing. The overall prevalence of Cryptosporidium was 25.6% (95% CI = 21.2% - 30.6%). Sequencing of the amplified fragments allowed for the identification of three species: C. meleagridis in 57 (62.6%), C. baileyi in 15 (16.4%), C. parvum in 3 (3.2%) samples, and a new Cryptosporidium genotype (C. genotype BrPR1) in 3 (3.2%) samples. Cryptosporidium genotype BrPR1 has not yet been classified as a species, and its host spectrum is not known. Cryptosporidium, including zoonotic species, exists at a high prevalence in free-range chickens within the region studied. |
| publisher |
Colégio Brasileiro de Parasitologia Veterinária |
| publishDate |
2017 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612017000400472 |
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