First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, Paraguay

First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, Paraguay

Abstract To verify the occurrence of natural Trypanosoma cruzi infection in non-human primates from a rural endemic area of the east region of Paraguay, xenodiagnosis was performed in 35 animals belonging to two species. For genotyping and T. cruzi discrete typing unit (DTU) assignment, a combination of four markers was used, including amplification products of the small (18S) and large (24Sα) subunits of ribosomal ribonucleic acid gene, the intergenic region of mini-exon gene and the heat shock protein 60 Eco-RV polymerase chain reaction-restriction fragment length polymorphism (HSP60/EcoRV-PCR-RFLP). One specimen of Sapajus cay was found positive and infected by the DTU TcII. This result constitutes the first record of natural T. cruzi infection in a sylvatic monkey in Paraguay, harbouring a DTU associated with severe Chagas disease in humans.

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Main Authors: Acosta,Nidia, Miret,Jorge, López,Elsa, Schinini,Alicia
Format: Digital revista
Language:English
Published: Colégio Brasileiro de Parasitologia Veterinária 2016
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000300327
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spelling oai:scielo:S1984-296120160003003272016-09-16First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, ParaguayAcosta,NidiaMiret,JorgeLópez,ElsaSchinini,Alicia Trypanosoma cruzi primates Paraguay Abstract To verify the occurrence of natural Trypanosoma cruzi infection in non-human primates from a rural endemic area of the east region of Paraguay, xenodiagnosis was performed in 35 animals belonging to two species. For genotyping and T. cruzi discrete typing unit (DTU) assignment, a combination of four markers was used, including amplification products of the small (18S) and large (24Sα) subunits of ribosomal ribonucleic acid gene, the intergenic region of mini-exon gene and the heat shock protein 60 Eco-RV polymerase chain reaction-restriction fragment length polymorphism (HSP60/EcoRV-PCR-RFLP). One specimen of Sapajus cay was found positive and infected by the DTU TcII. This result constitutes the first record of natural T. cruzi infection in a sylvatic monkey in Paraguay, harbouring a DTU associated with severe Chagas disease in humans.info:eu-repo/semantics/openAccessColégio Brasileiro de Parasitologia VeterináriaRevista Brasileira de Parasitologia Veterinária v.25 n.3 20162016-09-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000300327en10.1590/S1984-29612016052
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Acosta,Nidia
Miret,Jorge
López,Elsa
Schinini,Alicia
spellingShingle Acosta,Nidia
Miret,Jorge
López,Elsa
Schinini,Alicia
First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, Paraguay
author_facet Acosta,Nidia
Miret,Jorge
López,Elsa
Schinini,Alicia
author_sort Acosta,Nidia
title First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, Paraguay
title_short First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, Paraguay
title_full First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, Paraguay
title_fullStr First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, Paraguay
title_full_unstemmed First report of Sapajus cay naturally infected by Trypanosoma cruzi in San Pedro Department, Paraguay
title_sort first report of sapajus cay naturally infected by trypanosoma cruzi in san pedro department, paraguay
description Abstract To verify the occurrence of natural Trypanosoma cruzi infection in non-human primates from a rural endemic area of the east region of Paraguay, xenodiagnosis was performed in 35 animals belonging to two species. For genotyping and T. cruzi discrete typing unit (DTU) assignment, a combination of four markers was used, including amplification products of the small (18S) and large (24Sα) subunits of ribosomal ribonucleic acid gene, the intergenic region of mini-exon gene and the heat shock protein 60 Eco-RV polymerase chain reaction-restriction fragment length polymorphism (HSP60/EcoRV-PCR-RFLP). One specimen of Sapajus cay was found positive and infected by the DTU TcII. This result constitutes the first record of natural T. cruzi infection in a sylvatic monkey in Paraguay, harbouring a DTU associated with severe Chagas disease in humans.
publisher Colégio Brasileiro de Parasitologia Veterinária
publishDate 2016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612016000300327
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AT lopezelsa firstreportofsapajuscaynaturallyinfectedbytrypanosomacruziinsanpedrodepartmentparaguay
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