Molecular characterization reveals Brazilian Tomato chlorosis virus to be closely related to a Greek isolate
Tomato chlorosis virus (ToCV, genus Crinivirus, family Closteroviridae) is a whitefly-transmitted crinivirus with a bipartite RNA genome. This virus is emerging as a serious threat to tomato crops worldwide. To date, only three complete genomic sequences of ToCV have been described from North America, Spain, and Greece isolates. In this study, we present the fourth complete nucleotide sequence of the RNA 1 (8594 nt) and RNA 2 (8242 nt) components of a Brazilian ToCV isolate (ToCV-BR). The complete genome sequences of RNA 1 and RNA 2 have been deposited in the GenBank database under the accession numbers JQ952600 and JQ952601, respectively. The sequences of RNA 1 and RNA 2 shares the highest nucleotide identity of 99.6% and 99.5%, respectively, with the Greek isolate sequences. Phylogenetic analysis confirmed that both RNA 1 and RNA 2 of the Brazilian isolate are most closely related to the Greek isolate of that virus. These results suggest that ToCV may have been recently introduced to Brazil from Europe.
| Autores principales: | , , , , , |
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| Formato: | Digital revista |
| Idioma: | English |
| Publicado: |
Sociedade Brasileira de Fitopatologia
2013
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| Acceso en línea: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762013000400008 |
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