Spontaneous healing of the tympanic membrane after traumatic perforation in rats
OBJECTIVE: The objective of the present study was to assess the healing of traumatic tympanic membrane perforation in rats. METHODS: The tympanic membrane from male Wistar rats was perforated in the anterior and posterior portions to the handle of the malleus. Five tympanic membranes were evaluated 3 days after tympanic perforation; 5 after 5 days; 5 after 7 days; 3 after 10 days; and 4 after 14 days. The tympanic membranes were submitted to histopathological evaluation after hematoxylin-eosin staining. RESULTS: Tympanic membrane closure occurred at about 7-10 days after injury and the healing process was complete by day 14. The proliferative activity of the outer epithelial layer was present close to the handle of the malleus and to the tympanic annulus. CONCLUSION: The spontaneous healing process of the tympanic membrane starts from the outer epithelial layer, with later healing of the lamina propria and the mucosal layer.
Main Authors: | , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial.
2014
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942014000400330 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S1808-86942014000400330 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S1808-869420140004003302015-08-25Spontaneous healing of the tympanic membrane after traumatic perforation in rats Araújo,Marcos Miranda deMurashima,Adriana Andrade BatistaAlves,Vani MariaJamur,Maria CeliaHyppolito,Miguel Angelo Tympanic membrane perforation Wound healing Histology OBJECTIVE: The objective of the present study was to assess the healing of traumatic tympanic membrane perforation in rats. METHODS: The tympanic membrane from male Wistar rats was perforated in the anterior and posterior portions to the handle of the malleus. Five tympanic membranes were evaluated 3 days after tympanic perforation; 5 after 5 days; 5 after 7 days; 3 after 10 days; and 4 after 14 days. The tympanic membranes were submitted to histopathological evaluation after hematoxylin-eosin staining. RESULTS: Tympanic membrane closure occurred at about 7-10 days after injury and the healing process was complete by day 14. The proliferative activity of the outer epithelial layer was present close to the handle of the malleus and to the tympanic annulus. CONCLUSION: The spontaneous healing process of the tympanic membrane starts from the outer epithelial layer, with later healing of the lamina propria and the mucosal layer. info:eu-repo/semantics/openAccessAssociação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial.Brazilian Journal of Otorhinolaryngology v.80 n.4 20142014-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942014000400330en10.1016/j.bjorl.2014.05.014 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Araújo,Marcos Miranda de Murashima,Adriana Andrade Batista Alves,Vani Maria Jamur,Maria Celia Hyppolito,Miguel Angelo |
spellingShingle |
Araújo,Marcos Miranda de Murashima,Adriana Andrade Batista Alves,Vani Maria Jamur,Maria Celia Hyppolito,Miguel Angelo Spontaneous healing of the tympanic membrane after traumatic perforation in rats |
author_facet |
Araújo,Marcos Miranda de Murashima,Adriana Andrade Batista Alves,Vani Maria Jamur,Maria Celia Hyppolito,Miguel Angelo |
author_sort |
Araújo,Marcos Miranda de |
title |
Spontaneous healing of the tympanic membrane after traumatic perforation in rats |
title_short |
Spontaneous healing of the tympanic membrane after traumatic perforation in rats |
title_full |
Spontaneous healing of the tympanic membrane after traumatic perforation in rats |
title_fullStr |
Spontaneous healing of the tympanic membrane after traumatic perforation in rats |
title_full_unstemmed |
Spontaneous healing of the tympanic membrane after traumatic perforation in rats |
title_sort |
spontaneous healing of the tympanic membrane after traumatic perforation in rats |
description |
OBJECTIVE: The objective of the present study was to assess the healing of traumatic tympanic membrane perforation in rats. METHODS: The tympanic membrane from male Wistar rats was perforated in the anterior and posterior portions to the handle of the malleus. Five tympanic membranes were evaluated 3 days after tympanic perforation; 5 after 5 days; 5 after 7 days; 3 after 10 days; and 4 after 14 days. The tympanic membranes were submitted to histopathological evaluation after hematoxylin-eosin staining. RESULTS: Tympanic membrane closure occurred at about 7-10 days after injury and the healing process was complete by day 14. The proliferative activity of the outer epithelial layer was present close to the handle of the malleus and to the tympanic annulus. CONCLUSION: The spontaneous healing process of the tympanic membrane starts from the outer epithelial layer, with later healing of the lamina propria and the mucosal layer. |
publisher |
Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. |
publishDate |
2014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-86942014000400330 |
work_keys_str_mv |
AT araujomarcosmirandade spontaneoushealingofthetympanicmembraneaftertraumaticperforationinrats AT murashimaadrianaandradebatista spontaneoushealingofthetympanicmembraneaftertraumaticperforationinrats AT alvesvanimaria spontaneoushealingofthetympanicmembraneaftertraumaticperforationinrats AT jamurmariacelia spontaneoushealingofthetympanicmembraneaftertraumaticperforationinrats AT hyppolitomiguelangelo spontaneoushealingofthetympanicmembraneaftertraumaticperforationinrats |
_version_ |
1756433238054666240 |