Low occurrence of equine herpesvirus 1 (EHV-1) as cause of abortion and perinatal mortality in Brazil
ABSTRACT: Equine herpesvirus type 1 (EHV-1) is an important pathogen that causes abortion, neonatal disease, respiratory disorders, and neurological syndrome in equine populations worldwide. To evaluate EHV-1 as a cause of abortion and perinatal mortality in Brazil, tissue samples from 105 aborted equine fetuses, stillbirths, and foals up to one month of age were examined using virus isolation, immunohistochemistry (IHC), histopathology, and nested polymerase chain reaction (PCR). Two fetuses were positive for EHV-1 by PCR, one of which showed syncytia and eosinophilic intranuclear inclusion bodies in bronchial epithelia, but it was negative by virus isolation. The other showed no characteristic histological lesions, but it was positive by viral isolation. No sample was positive by IHC. The results presented low occurrence of EHV-1 in the studied population and suggested that the use of a combination of techniques increases the likelihood of an accurate diagnosis of EHV-1.
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Instituto Biológico
2018
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oai:scielo:S1808-165720180001002222023-01-09Low occurrence of equine herpesvirus 1 (EHV-1) as cause of abortion and perinatal mortality in BrazilSilva,Aline Aparecida daCunha,Elenice Maria SequetinLara,Maria do Carmo Custódio de Souza HunoldVillalobos,Eliana Monteforte CassaroNassar,Alessandra Figueiredo de CastroMori,EnioZanuzzi,Carolina NataliaGalosi,Cecília MônicaFava,Claudia Del equine herpesvirus type 1 histopathology immunohistochemistry polymerase chain reaction virus isolation ABSTRACT: Equine herpesvirus type 1 (EHV-1) is an important pathogen that causes abortion, neonatal disease, respiratory disorders, and neurological syndrome in equine populations worldwide. To evaluate EHV-1 as a cause of abortion and perinatal mortality in Brazil, tissue samples from 105 aborted equine fetuses, stillbirths, and foals up to one month of age were examined using virus isolation, immunohistochemistry (IHC), histopathology, and nested polymerase chain reaction (PCR). Two fetuses were positive for EHV-1 by PCR, one of which showed syncytia and eosinophilic intranuclear inclusion bodies in bronchial epithelia, but it was negative by virus isolation. The other showed no characteristic histological lesions, but it was positive by viral isolation. No sample was positive by IHC. The results presented low occurrence of EHV-1 in the studied population and suggested that the use of a combination of techniques increases the likelihood of an accurate diagnosis of EHV-1.info:eu-repo/semantics/openAccessInstituto BiológicoArquivos do Instituto Biológico v.85 20182018-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100222en10.1590/1808-1657000852017 |
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Silva,Aline Aparecida da Cunha,Elenice Maria Sequetin Lara,Maria do Carmo Custódio de Souza Hunold Villalobos,Eliana Monteforte Cassaro Nassar,Alessandra Figueiredo de Castro Mori,Enio Zanuzzi,Carolina Natalia Galosi,Cecília Mônica Fava,Claudia Del |
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Silva,Aline Aparecida da Cunha,Elenice Maria Sequetin Lara,Maria do Carmo Custódio de Souza Hunold Villalobos,Eliana Monteforte Cassaro Nassar,Alessandra Figueiredo de Castro Mori,Enio Zanuzzi,Carolina Natalia Galosi,Cecília Mônica Fava,Claudia Del Low occurrence of equine herpesvirus 1 (EHV-1) as cause of abortion and perinatal mortality in Brazil |
author_facet |
Silva,Aline Aparecida da Cunha,Elenice Maria Sequetin Lara,Maria do Carmo Custódio de Souza Hunold Villalobos,Eliana Monteforte Cassaro Nassar,Alessandra Figueiredo de Castro Mori,Enio Zanuzzi,Carolina Natalia Galosi,Cecília Mônica Fava,Claudia Del |
author_sort |
Silva,Aline Aparecida da |
title |
Low occurrence of equine herpesvirus 1 (EHV-1) as cause of abortion and perinatal mortality in Brazil |
title_short |
Low occurrence of equine herpesvirus 1 (EHV-1) as cause of abortion and perinatal mortality in Brazil |
title_full |
Low occurrence of equine herpesvirus 1 (EHV-1) as cause of abortion and perinatal mortality in Brazil |
title_fullStr |
Low occurrence of equine herpesvirus 1 (EHV-1) as cause of abortion and perinatal mortality in Brazil |
title_full_unstemmed |
Low occurrence of equine herpesvirus 1 (EHV-1) as cause of abortion and perinatal mortality in Brazil |
title_sort |
low occurrence of equine herpesvirus 1 (ehv-1) as cause of abortion and perinatal mortality in brazil |
description |
ABSTRACT: Equine herpesvirus type 1 (EHV-1) is an important pathogen that causes abortion, neonatal disease, respiratory disorders, and neurological syndrome in equine populations worldwide. To evaluate EHV-1 as a cause of abortion and perinatal mortality in Brazil, tissue samples from 105 aborted equine fetuses, stillbirths, and foals up to one month of age were examined using virus isolation, immunohistochemistry (IHC), histopathology, and nested polymerase chain reaction (PCR). Two fetuses were positive for EHV-1 by PCR, one of which showed syncytia and eosinophilic intranuclear inclusion bodies in bronchial epithelia, but it was negative by virus isolation. The other showed no characteristic histological lesions, but it was positive by viral isolation. No sample was positive by IHC. The results presented low occurrence of EHV-1 in the studied population and suggested that the use of a combination of techniques increases the likelihood of an accurate diagnosis of EHV-1. |
publisher |
Instituto Biológico |
publishDate |
2018 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572018000100222 |
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