Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines

Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines

ABSTRACT. The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot length was significantly lower under dry conditions. Through transcript analysis, the genes ZmDBP3 and ZmAN13 were identified as potential markers for the early selection of maize lines tolerant to water deficit.

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Main Authors: Marques,Thaís Lima, Pinho,Renzo Garcia Von, Pinho,Édila Vilela de Resende Von, Paniago,Bruno da Costa, Freitas,Natália Chagas, Santos,Heloisa Oliveira dos
Format: Digital revista
Language:English
Published: Editora da Universidade Estadual de Maringá - EDUEM 2020
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86212020000102006
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spelling oai:scielo:S1807-862120200001020062020-01-22Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize linesMarques,Thaís LimaPinho,Renzo Garcia VonPinho,Édila Vilela de Resende VonPaniago,Bruno da CostaFreitas,Natália ChagasSantos,Heloisa Oliveira dos abiotic stress Zea mays qRT-PCR water deficit ABSTRACT. The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot length was significantly lower under dry conditions. Through transcript analysis, the genes ZmDBP3 and ZmAN13 were identified as potential markers for the early selection of maize lines tolerant to water deficit.info:eu-repo/semantics/openAccessEditora da Universidade Estadual de Maringá - EDUEMActa Scientiarum. Agronomy v.42 20202020-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86212020000102006en10.4025/actasciagron.v42i1.43479
institution SCIELO
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country Brasil
countrycode BR
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databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Marques,Thaís Lima
Pinho,Renzo Garcia Von
Pinho,Édila Vilela de Resende Von
Paniago,Bruno da Costa
Freitas,Natália Chagas
Santos,Heloisa Oliveira dos
spellingShingle Marques,Thaís Lima
Pinho,Renzo Garcia Von
Pinho,Édila Vilela de Resende Von
Paniago,Bruno da Costa
Freitas,Natália Chagas
Santos,Heloisa Oliveira dos
Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
author_facet Marques,Thaís Lima
Pinho,Renzo Garcia Von
Pinho,Édila Vilela de Resende Von
Paniago,Bruno da Costa
Freitas,Natália Chagas
Santos,Heloisa Oliveira dos
author_sort Marques,Thaís Lima
title Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
title_short Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
title_full Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
title_fullStr Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
title_full_unstemmed Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
title_sort physiological analysis and gene expression analysis of zmdbp3, zmaldh9, zman13, and zmdreb2a in maize lines
description ABSTRACT. The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot length was significantly lower under dry conditions. Through transcript analysis, the genes ZmDBP3 and ZmAN13 were identified as potential markers for the early selection of maize lines tolerant to water deficit.
publisher Editora da Universidade Estadual de Maringá - EDUEM
publishDate 2020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1807-86212020000102006
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