Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds

Soybean oil contains around 60 % of polyunsaturated fatty acids, which are responsible for the low oxidative stability of soy-derived products. Soybean lines with low linolenic acid content can be obtained by genetic manipulation; however, a high proportion of the variation in fatty acids content is due to environmental factors. This work aimed to determine the effect of temperature on oil composition and on the activity of the enzymes CDP-choline:1,2-diacylglycerolcholine phosphotransferase (CPT) and acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), responsible for maintenance of polyunsaturated fatty acids in the cytoplasmic acyl-CoA pool, that is used for oil synthesis in the seeds. CAC-1, a soybean variety with linolenic acid content of about 8 % and CC4, a BC3F4 CAC-1 derived line, with about 4 % linolenic acid, were used. The lines were cultivated under two temperature: 34/28ºC or 22/13ºC - day/night. The seeds were collected along seven development stages, according to their fresh weight. Fatty acid analysis was carried out by gas chromatography and CPT and LPCAT activities were determined by measuring the radioactivity incorporated in their products, phosphatidyl-[14C]choline and phosphatidylcholine-[14C]oleoyl, respectively. Linolenic acid contents were 3.89 and 6.92 % for line CC4 and 7.39 and 12.49 % for variety CAC-1, when submitted to high and low temperature conditions, respectively. Both enzymes were more active, in the development stages analyzed, in seeds produced under low temperature. Kinetics characterization of CPT and LPCAT were conducted previously.

Saved in:
Bibliographic Details
Main Authors: Lanna,Anna Cristina, José,Inês Chamel, Oliveira,Maria Goreti de Almeida, Barros,Everaldo Gonçalves, Moreira,Maurilio Alves
Format: Digital revista
Language:English
Published: Brazilian Journal of Plant Physiology 2005
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200004
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S1677-04202005000200004
record_format ojs
spelling oai:scielo:S1677-042020050002000042005-09-01Effect of temperature on polyunsaturated fatty acid accumulation in soybean seedsLanna,Anna CristinaJosé,Inês ChamelOliveira,Maria Goreti de AlmeidaBarros,Everaldo GonçalvesMoreira,Maurilio Alves Cholinephosphotransferase kinetic characterization linoleic acid linolenic acid lysophosphatidylcholine acyltransferase soybean seed Soybean oil contains around 60 % of polyunsaturated fatty acids, which are responsible for the low oxidative stability of soy-derived products. Soybean lines with low linolenic acid content can be obtained by genetic manipulation; however, a high proportion of the variation in fatty acids content is due to environmental factors. This work aimed to determine the effect of temperature on oil composition and on the activity of the enzymes CDP-choline:1,2-diacylglycerolcholine phosphotransferase (CPT) and acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), responsible for maintenance of polyunsaturated fatty acids in the cytoplasmic acyl-CoA pool, that is used for oil synthesis in the seeds. CAC-1, a soybean variety with linolenic acid content of about 8 % and CC4, a BC3F4 CAC-1 derived line, with about 4 % linolenic acid, were used. The lines were cultivated under two temperature: 34/28ºC or 22/13ºC - day/night. The seeds were collected along seven development stages, according to their fresh weight. Fatty acid analysis was carried out by gas chromatography and CPT and LPCAT activities were determined by measuring the radioactivity incorporated in their products, phosphatidyl-[14C]choline and phosphatidylcholine-[14C]oleoyl, respectively. Linolenic acid contents were 3.89 and 6.92 % for line CC4 and 7.39 and 12.49 % for variety CAC-1, when submitted to high and low temperature conditions, respectively. Both enzymes were more active, in the development stages analyzed, in seeds produced under low temperature. Kinetics characterization of CPT and LPCAT were conducted previously.info:eu-repo/semantics/openAccessBrazilian Journal of Plant PhysiologyBrazilian Journal of Plant Physiology v.17 n.2 20052005-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200004en10.1590/S1677-04202005000200004
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Lanna,Anna Cristina
José,Inês Chamel
Oliveira,Maria Goreti de Almeida
Barros,Everaldo Gonçalves
Moreira,Maurilio Alves
spellingShingle Lanna,Anna Cristina
José,Inês Chamel
Oliveira,Maria Goreti de Almeida
Barros,Everaldo Gonçalves
Moreira,Maurilio Alves
Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds
author_facet Lanna,Anna Cristina
José,Inês Chamel
Oliveira,Maria Goreti de Almeida
Barros,Everaldo Gonçalves
Moreira,Maurilio Alves
author_sort Lanna,Anna Cristina
title Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds
title_short Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds
title_full Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds
title_fullStr Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds
title_full_unstemmed Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds
title_sort effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds
description Soybean oil contains around 60 % of polyunsaturated fatty acids, which are responsible for the low oxidative stability of soy-derived products. Soybean lines with low linolenic acid content can be obtained by genetic manipulation; however, a high proportion of the variation in fatty acids content is due to environmental factors. This work aimed to determine the effect of temperature on oil composition and on the activity of the enzymes CDP-choline:1,2-diacylglycerolcholine phosphotransferase (CPT) and acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), responsible for maintenance of polyunsaturated fatty acids in the cytoplasmic acyl-CoA pool, that is used for oil synthesis in the seeds. CAC-1, a soybean variety with linolenic acid content of about 8 % and CC4, a BC3F4 CAC-1 derived line, with about 4 % linolenic acid, were used. The lines were cultivated under two temperature: 34/28ºC or 22/13ºC - day/night. The seeds were collected along seven development stages, according to their fresh weight. Fatty acid analysis was carried out by gas chromatography and CPT and LPCAT activities were determined by measuring the radioactivity incorporated in their products, phosphatidyl-[14C]choline and phosphatidylcholine-[14C]oleoyl, respectively. Linolenic acid contents were 3.89 and 6.92 % for line CC4 and 7.39 and 12.49 % for variety CAC-1, when submitted to high and low temperature conditions, respectively. Both enzymes were more active, in the development stages analyzed, in seeds produced under low temperature. Kinetics characterization of CPT and LPCAT were conducted previously.
publisher Brazilian Journal of Plant Physiology
publishDate 2005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200004
work_keys_str_mv AT lannaannacristina effectoftemperatureonpolyunsaturatedfattyacidaccumulationinsoybeanseeds
AT joseineschamel effectoftemperatureonpolyunsaturatedfattyacidaccumulationinsoybeanseeds
AT oliveiramariagoretidealmeida effectoftemperatureonpolyunsaturatedfattyacidaccumulationinsoybeanseeds
AT barroseveraldogoncalves effectoftemperatureonpolyunsaturatedfattyacidaccumulationinsoybeanseeds
AT moreiramaurilioalves effectoftemperatureonpolyunsaturatedfattyacidaccumulationinsoybeanseeds
_version_ 1756427659763515392