Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds
Soybean oil contains around 60 % of polyunsaturated fatty acids, which are responsible for the low oxidative stability of soy-derived products. Soybean lines with low linolenic acid content can be obtained by genetic manipulation; however, a high proportion of the variation in fatty acids content is due to environmental factors. This work aimed to determine the effect of temperature on oil composition and on the activity of the enzymes CDP-choline:1,2-diacylglycerolcholine phosphotransferase (CPT) and acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), responsible for maintenance of polyunsaturated fatty acids in the cytoplasmic acyl-CoA pool, that is used for oil synthesis in the seeds. CAC-1, a soybean variety with linolenic acid content of about 8 % and CC4, a BC3F4 CAC-1 derived line, with about 4 % linolenic acid, were used. The lines were cultivated under two temperature: 34/28ºC or 22/13ºC - day/night. The seeds were collected along seven development stages, according to their fresh weight. Fatty acid analysis was carried out by gas chromatography and CPT and LPCAT activities were determined by measuring the radioactivity incorporated in their products, phosphatidyl-[14C]choline and phosphatidylcholine-[14C]oleoyl, respectively. Linolenic acid contents were 3.89 and 6.92 % for line CC4 and 7.39 and 12.49 % for variety CAC-1, when submitted to high and low temperature conditions, respectively. Both enzymes were more active, in the development stages analyzed, in seeds produced under low temperature. Kinetics characterization of CPT and LPCAT were conducted previously.
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Brazilian Journal of Plant Physiology
2005
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oai:scielo:S1677-042020050002000042005-09-01Effect of temperature on polyunsaturated fatty acid accumulation in soybean seedsLanna,Anna CristinaJosé,Inês ChamelOliveira,Maria Goreti de AlmeidaBarros,Everaldo GonçalvesMoreira,Maurilio Alves Cholinephosphotransferase kinetic characterization linoleic acid linolenic acid lysophosphatidylcholine acyltransferase soybean seed Soybean oil contains around 60 % of polyunsaturated fatty acids, which are responsible for the low oxidative stability of soy-derived products. Soybean lines with low linolenic acid content can be obtained by genetic manipulation; however, a high proportion of the variation in fatty acids content is due to environmental factors. This work aimed to determine the effect of temperature on oil composition and on the activity of the enzymes CDP-choline:1,2-diacylglycerolcholine phosphotransferase (CPT) and acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), responsible for maintenance of polyunsaturated fatty acids in the cytoplasmic acyl-CoA pool, that is used for oil synthesis in the seeds. CAC-1, a soybean variety with linolenic acid content of about 8 % and CC4, a BC3F4 CAC-1 derived line, with about 4 % linolenic acid, were used. The lines were cultivated under two temperature: 34/28ºC or 22/13ºC - day/night. The seeds were collected along seven development stages, according to their fresh weight. Fatty acid analysis was carried out by gas chromatography and CPT and LPCAT activities were determined by measuring the radioactivity incorporated in their products, phosphatidyl-[14C]choline and phosphatidylcholine-[14C]oleoyl, respectively. Linolenic acid contents were 3.89 and 6.92 % for line CC4 and 7.39 and 12.49 % for variety CAC-1, when submitted to high and low temperature conditions, respectively. Both enzymes were more active, in the development stages analyzed, in seeds produced under low temperature. Kinetics characterization of CPT and LPCAT were conducted previously.info:eu-repo/semantics/openAccessBrazilian Journal of Plant PhysiologyBrazilian Journal of Plant Physiology v.17 n.2 20052005-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200004en10.1590/S1677-04202005000200004 |
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Lanna,Anna Cristina José,Inês Chamel Oliveira,Maria Goreti de Almeida Barros,Everaldo Gonçalves Moreira,Maurilio Alves |
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Lanna,Anna Cristina José,Inês Chamel Oliveira,Maria Goreti de Almeida Barros,Everaldo Gonçalves Moreira,Maurilio Alves Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds |
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Lanna,Anna Cristina José,Inês Chamel Oliveira,Maria Goreti de Almeida Barros,Everaldo Gonçalves Moreira,Maurilio Alves |
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Lanna,Anna Cristina |
title |
Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds |
title_short |
Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds |
title_full |
Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds |
title_fullStr |
Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds |
title_full_unstemmed |
Effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds |
title_sort |
effect of temperature on polyunsaturated fatty acid accumulation in soybean seeds |
description |
Soybean oil contains around 60 % of polyunsaturated fatty acids, which are responsible for the low oxidative stability of soy-derived products. Soybean lines with low linolenic acid content can be obtained by genetic manipulation; however, a high proportion of the variation in fatty acids content is due to environmental factors. This work aimed to determine the effect of temperature on oil composition and on the activity of the enzymes CDP-choline:1,2-diacylglycerolcholine phosphotransferase (CPT) and acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), responsible for maintenance of polyunsaturated fatty acids in the cytoplasmic acyl-CoA pool, that is used for oil synthesis in the seeds. CAC-1, a soybean variety with linolenic acid content of about 8 % and CC4, a BC3F4 CAC-1 derived line, with about 4 % linolenic acid, were used. The lines were cultivated under two temperature: 34/28ºC or 22/13ºC - day/night. The seeds were collected along seven development stages, according to their fresh weight. Fatty acid analysis was carried out by gas chromatography and CPT and LPCAT activities were determined by measuring the radioactivity incorporated in their products, phosphatidyl-[14C]choline and phosphatidylcholine-[14C]oleoyl, respectively. Linolenic acid contents were 3.89 and 6.92 % for line CC4 and 7.39 and 12.49 % for variety CAC-1, when submitted to high and low temperature conditions, respectively. Both enzymes were more active, in the development stages analyzed, in seeds produced under low temperature. Kinetics characterization of CPT and LPCAT were conducted previously. |
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Brazilian Journal of Plant Physiology |
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2005 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200004 |
work_keys_str_mv |
AT lannaannacristina effectoftemperatureonpolyunsaturatedfattyacidaccumulationinsoybeanseeds AT joseineschamel effectoftemperatureonpolyunsaturatedfattyacidaccumulationinsoybeanseeds AT oliveiramariagoretidealmeida effectoftemperatureonpolyunsaturatedfattyacidaccumulationinsoybeanseeds AT barroseveraldogoncalves effectoftemperatureonpolyunsaturatedfattyacidaccumulationinsoybeanseeds AT moreiramaurilioalves effectoftemperatureonpolyunsaturatedfattyacidaccumulationinsoybeanseeds |
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