BRAF mutations in cutaneous melanoma: no correlation with histological prognostic factors or overall survival
INTRODUCTION: Molecular biology techniques allow identification of molecular markers such as BRAF and c-Kit gene mutations in melanomas. Studies on genetic alterations in melanomas of South-American patients are sparse. OBJECTIVES: To identify the incidence of BRAF and c-Kit gene mutations in primary cutaneous melanomas in Brazilian patients and to evaluate pathogenetic and prognostic implications of these mutations correlating them with clinical and histopathological data. MATERIAL AND METHODS: Ninety-six surgical specimens of primary cutaneous melanoma and 15 corresponding metastasis were analyzed using TaqMan Real-Time polymerase chain reaction (PCR) assays. RESULTS: In comparison with the medical literature, a relatively low frequency of BRAF mutation in primary (39%) and metastatic (40%) melanomas and complete absence of c-Kit gene mutations were demonstrated. BRAF mutations arose at an early stage during melanoma progression and were not involved in the transition of thin (< 1 mm) to thick (> 1 mm) melanomas. BRAF mutations are related to patients' younger age and to the pattern of sun exposure, although there was no correlation with any histological prognostic factor or overall survival. CONCLUSION: The identification of both BRAF and c-Kit mutation is not a suitable prognostic indicator in the Brazilian population. Moreover, the relatively low frequency of BRAF mutations brings into question if it actually plays a key role in melanoma pathogenesis.
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Sociedade Brasileira de Patologia Clínica
2010
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oai:scielo:S1676-244420100006000092011-01-05BRAF mutations in cutaneous melanoma: no correlation with histological prognostic factors or overall survivalJung,Juliana ElizabethFalk,Thomas M.Bresch,MartinaMatias,Jorge Eduardo FoutoBöer,Almut Proto-oncogene proteins BRAF Proto-oncogene proteins c-Kit Malignant melanoma INTRODUCTION: Molecular biology techniques allow identification of molecular markers such as BRAF and c-Kit gene mutations in melanomas. Studies on genetic alterations in melanomas of South-American patients are sparse. OBJECTIVES: To identify the incidence of BRAF and c-Kit gene mutations in primary cutaneous melanomas in Brazilian patients and to evaluate pathogenetic and prognostic implications of these mutations correlating them with clinical and histopathological data. MATERIAL AND METHODS: Ninety-six surgical specimens of primary cutaneous melanoma and 15 corresponding metastasis were analyzed using TaqMan Real-Time polymerase chain reaction (PCR) assays. RESULTS: In comparison with the medical literature, a relatively low frequency of BRAF mutation in primary (39%) and metastatic (40%) melanomas and complete absence of c-Kit gene mutations were demonstrated. BRAF mutations arose at an early stage during melanoma progression and were not involved in the transition of thin (< 1 mm) to thick (> 1 mm) melanomas. BRAF mutations are related to patients' younger age and to the pattern of sun exposure, although there was no correlation with any histological prognostic factor or overall survival. CONCLUSION: The identification of both BRAF and c-Kit mutation is not a suitable prognostic indicator in the Brazilian population. Moreover, the relatively low frequency of BRAF mutations brings into question if it actually plays a key role in melanoma pathogenesis.info:eu-repo/semantics/openAccessSociedade Brasileira de Patologia Clínica Jornal Brasileiro de Patologia e Medicina Laboratorial v.46 n.6 20102010-12-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442010000600009en10.1590/S1676-24442010000600009 |
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Jung,Juliana Elizabeth Falk,Thomas M. Bresch,Martina Matias,Jorge Eduardo Fouto Böer,Almut |
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Jung,Juliana Elizabeth Falk,Thomas M. Bresch,Martina Matias,Jorge Eduardo Fouto Böer,Almut BRAF mutations in cutaneous melanoma: no correlation with histological prognostic factors or overall survival |
author_facet |
Jung,Juliana Elizabeth Falk,Thomas M. Bresch,Martina Matias,Jorge Eduardo Fouto Böer,Almut |
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Jung,Juliana Elizabeth |
title |
BRAF mutations in cutaneous melanoma: no correlation with histological prognostic factors or overall survival |
title_short |
BRAF mutations in cutaneous melanoma: no correlation with histological prognostic factors or overall survival |
title_full |
BRAF mutations in cutaneous melanoma: no correlation with histological prognostic factors or overall survival |
title_fullStr |
BRAF mutations in cutaneous melanoma: no correlation with histological prognostic factors or overall survival |
title_full_unstemmed |
BRAF mutations in cutaneous melanoma: no correlation with histological prognostic factors or overall survival |
title_sort |
braf mutations in cutaneous melanoma: no correlation with histological prognostic factors or overall survival |
description |
INTRODUCTION: Molecular biology techniques allow identification of molecular markers such as BRAF and c-Kit gene mutations in melanomas. Studies on genetic alterations in melanomas of South-American patients are sparse. OBJECTIVES: To identify the incidence of BRAF and c-Kit gene mutations in primary cutaneous melanomas in Brazilian patients and to evaluate pathogenetic and prognostic implications of these mutations correlating them with clinical and histopathological data. MATERIAL AND METHODS: Ninety-six surgical specimens of primary cutaneous melanoma and 15 corresponding metastasis were analyzed using TaqMan Real-Time polymerase chain reaction (PCR) assays. RESULTS: In comparison with the medical literature, a relatively low frequency of BRAF mutation in primary (39%) and metastatic (40%) melanomas and complete absence of c-Kit gene mutations were demonstrated. BRAF mutations arose at an early stage during melanoma progression and were not involved in the transition of thin (< 1 mm) to thick (> 1 mm) melanomas. BRAF mutations are related to patients' younger age and to the pattern of sun exposure, although there was no correlation with any histological prognostic factor or overall survival. CONCLUSION: The identification of both BRAF and c-Kit mutation is not a suitable prognostic indicator in the Brazilian population. Moreover, the relatively low frequency of BRAF mutations brings into question if it actually plays a key role in melanoma pathogenesis. |
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Sociedade Brasileira de Patologia Clínica |
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2010 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-24442010000600009 |
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1756427470421098496 |