Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae

Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae

Microsatellite markers previously developed for Pitcairnia albiflos Herb. and Pitcairnia geysksii L.B.Sm. were used in cross-amplification tests of five other Bromeliaceae species. Ten (76.9%) out of the 13 evaluated pair of primers had positive results for some of the species tested. The number of polymorphic alleles ranged between two and four in most species. Ho values ranged between zero, in Pitcairnia flammea Lindl. (PaA05), Aechmea ramosa Mart ex Schult & Schult and Billbergia horrida Regel (PaC05), and one in Billbergia euphemiae E. Morren (PaA05, PaA10, PaC05 and PaD07). This study showed that microsatellite markers developed for P. albiflos and P. geysksii effectively amplified the DNA samples of Pitcairnia flammea, Aechmea nudicaulis (L.) Griseb., Aechmea ramosa, Billbergia horrida and Billbergia euphemiae, validating the transferability of these markers to species of the Pitcairnioideae and Bromelioideae subfamilies.

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Main Authors: Miranda,Fabio Demolinari de, Gontijo,Andreia Barcelos Passos Lima, Santiliano,Fabiano Costa, Favoreto,Fernanda Campanharo, Soares,Taís Cristina Bastos
Format: Digital revista
Language:English
Published: Instituto Virtual da Biodiversidade | BIOTA - FAPESP 2012
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300032
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spelling oai:scielo:S1676-060320120003000322012-11-08Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and BromelioideaeMiranda,Fabio Demolinari deGontijo,Andreia Barcelos Passos LimaSantiliano,Fabiano CostaFavoreto,Fernanda CampanharoSoares,Taís Cristina Bastos cross-amplification SSR genetic diversity bromeliads conservation Microsatellite markers previously developed for Pitcairnia albiflos Herb. and Pitcairnia geysksii L.B.Sm. were used in cross-amplification tests of five other Bromeliaceae species. Ten (76.9%) out of the 13 evaluated pair of primers had positive results for some of the species tested. The number of polymorphic alleles ranged between two and four in most species. Ho values ranged between zero, in Pitcairnia flammea Lindl. (PaA05), Aechmea ramosa Mart ex Schult & Schult and Billbergia horrida Regel (PaC05), and one in Billbergia euphemiae E. Morren (PaA05, PaA10, PaC05 and PaD07). This study showed that microsatellite markers developed for P. albiflos and P. geysksii effectively amplified the DNA samples of Pitcairnia flammea, Aechmea nudicaulis (L.) Griseb., Aechmea ramosa, Billbergia horrida and Billbergia euphemiae, validating the transferability of these markers to species of the Pitcairnioideae and Bromelioideae subfamilies.info:eu-repo/semantics/openAccessInstituto Virtual da Biodiversidade | BIOTA - FAPESPBiota Neotropica v.12 n.3 20122012-09-01info:eu-repo/semantics/othertext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300032en10.1590/S1676-06032012000300032
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Miranda,Fabio Demolinari de
Gontijo,Andreia Barcelos Passos Lima
Santiliano,Fabiano Costa
Favoreto,Fernanda Campanharo
Soares,Taís Cristina Bastos
spellingShingle Miranda,Fabio Demolinari de
Gontijo,Andreia Barcelos Passos Lima
Santiliano,Fabiano Costa
Favoreto,Fernanda Campanharo
Soares,Taís Cristina Bastos
Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae
author_facet Miranda,Fabio Demolinari de
Gontijo,Andreia Barcelos Passos Lima
Santiliano,Fabiano Costa
Favoreto,Fernanda Campanharo
Soares,Taís Cristina Bastos
author_sort Miranda,Fabio Demolinari de
title Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae
title_short Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae
title_full Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae
title_fullStr Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae
title_full_unstemmed Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae
title_sort transferability and characterization of microsatellite markers in five bromeliaceae species belonging to the subfamilies pitcairnioideae and bromelioideae
description Microsatellite markers previously developed for Pitcairnia albiflos Herb. and Pitcairnia geysksii L.B.Sm. were used in cross-amplification tests of five other Bromeliaceae species. Ten (76.9%) out of the 13 evaluated pair of primers had positive results for some of the species tested. The number of polymorphic alleles ranged between two and four in most species. Ho values ranged between zero, in Pitcairnia flammea Lindl. (PaA05), Aechmea ramosa Mart ex Schult & Schult and Billbergia horrida Regel (PaC05), and one in Billbergia euphemiae E. Morren (PaA05, PaA10, PaC05 and PaD07). This study showed that microsatellite markers developed for P. albiflos and P. geysksii effectively amplified the DNA samples of Pitcairnia flammea, Aechmea nudicaulis (L.) Griseb., Aechmea ramosa, Billbergia horrida and Billbergia euphemiae, validating the transferability of these markers to species of the Pitcairnioideae and Bromelioideae subfamilies.
publisher Instituto Virtual da Biodiversidade | BIOTA - FAPESP
publishDate 2012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300032
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