Evaluation of Microbiological Cloth and Sponge Disinfection Methods in a Hospital’s Food and Nutrition Unit

Abstract Food safety, within the context of the hospital’s Food and Nutrition Units (FNU), is one of the key factors in ensuring the quality of the service. Thus, the aim of this study was to analyze the microbiological contamination of sponges and multipurpose cloths used in a hospital’s FNU, as well as the effectiveness of different disinfection methods. The materials were submitted to viable cell count for total and thermotolerant coliforms, coagulase-positive Staphylococcus, and mesophiles; in addition, efficiency tests of the disinfection methods carried out by the FNU were done by scanning electron microscopy, antimicrobial susceptibility testing, biofilm formation capacity, and virulence factors. The analysis results confirmed the contamination of these utensils, and the disinfection methods used were efficient. Electromicroscopic analysis identified high bacterial adhesion after use, and the isolates were resistant to the antimicrobial penicillin and classified as poor biofilm producers. Sponge isolates showed greater virulence than other materials. Therefore, good practices in food services must follow strict hygiene and disinfection protocols to ensure the safety of the food produced.

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Main Authors: Almeida,Ana Paula Maia, Rosa,Gabriela Rosa da, Blumberg,Thayane Garcia, Marques,Luany Oliveira, Bordini,Fernanda Weber, Rosolen,Michele Dutra, Oliveira,Jordan Teixeira, Pieniz,Simone
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2022
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100327
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spelling oai:scielo:S1516-891320220001003272022-07-20Evaluation of Microbiological Cloth and Sponge Disinfection Methods in a Hospital’s Food and Nutrition UnitAlmeida,Ana Paula MaiaRosa,Gabriela Rosa daBlumberg,Thayane GarciaMarques,Luany OliveiraBordini,Fernanda WeberRosolen,Michele DutraOliveira,Jordan TeixeiraPieniz,Simone Sanitation Disinfection Food safety Foodborne Diseases. Abstract Food safety, within the context of the hospital’s Food and Nutrition Units (FNU), is one of the key factors in ensuring the quality of the service. Thus, the aim of this study was to analyze the microbiological contamination of sponges and multipurpose cloths used in a hospital’s FNU, as well as the effectiveness of different disinfection methods. The materials were submitted to viable cell count for total and thermotolerant coliforms, coagulase-positive Staphylococcus, and mesophiles; in addition, efficiency tests of the disinfection methods carried out by the FNU were done by scanning electron microscopy, antimicrobial susceptibility testing, biofilm formation capacity, and virulence factors. The analysis results confirmed the contamination of these utensils, and the disinfection methods used were efficient. Electromicroscopic analysis identified high bacterial adhesion after use, and the isolates were resistant to the antimicrobial penicillin and classified as poor biofilm producers. Sponge isolates showed greater virulence than other materials. Therefore, good practices in food services must follow strict hygiene and disinfection protocols to ensure the safety of the food produced.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.65 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100327en10.1590/1678-4324-2022210551
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language English
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author Almeida,Ana Paula Maia
Rosa,Gabriela Rosa da
Blumberg,Thayane Garcia
Marques,Luany Oliveira
Bordini,Fernanda Weber
Rosolen,Michele Dutra
Oliveira,Jordan Teixeira
Pieniz,Simone
spellingShingle Almeida,Ana Paula Maia
Rosa,Gabriela Rosa da
Blumberg,Thayane Garcia
Marques,Luany Oliveira
Bordini,Fernanda Weber
Rosolen,Michele Dutra
Oliveira,Jordan Teixeira
Pieniz,Simone
Evaluation of Microbiological Cloth and Sponge Disinfection Methods in a Hospital’s Food and Nutrition Unit
author_facet Almeida,Ana Paula Maia
Rosa,Gabriela Rosa da
Blumberg,Thayane Garcia
Marques,Luany Oliveira
Bordini,Fernanda Weber
Rosolen,Michele Dutra
Oliveira,Jordan Teixeira
Pieniz,Simone
author_sort Almeida,Ana Paula Maia
title Evaluation of Microbiological Cloth and Sponge Disinfection Methods in a Hospital’s Food and Nutrition Unit
title_short Evaluation of Microbiological Cloth and Sponge Disinfection Methods in a Hospital’s Food and Nutrition Unit
title_full Evaluation of Microbiological Cloth and Sponge Disinfection Methods in a Hospital’s Food and Nutrition Unit
title_fullStr Evaluation of Microbiological Cloth and Sponge Disinfection Methods in a Hospital’s Food and Nutrition Unit
title_full_unstemmed Evaluation of Microbiological Cloth and Sponge Disinfection Methods in a Hospital’s Food and Nutrition Unit
title_sort evaluation of microbiological cloth and sponge disinfection methods in a hospital’s food and nutrition unit
description Abstract Food safety, within the context of the hospital’s Food and Nutrition Units (FNU), is one of the key factors in ensuring the quality of the service. Thus, the aim of this study was to analyze the microbiological contamination of sponges and multipurpose cloths used in a hospital’s FNU, as well as the effectiveness of different disinfection methods. The materials were submitted to viable cell count for total and thermotolerant coliforms, coagulase-positive Staphylococcus, and mesophiles; in addition, efficiency tests of the disinfection methods carried out by the FNU were done by scanning electron microscopy, antimicrobial susceptibility testing, biofilm formation capacity, and virulence factors. The analysis results confirmed the contamination of these utensils, and the disinfection methods used were efficient. Electromicroscopic analysis identified high bacterial adhesion after use, and the isolates were resistant to the antimicrobial penicillin and classified as poor biofilm producers. Sponge isolates showed greater virulence than other materials. Therefore, good practices in food services must follow strict hygiene and disinfection protocols to ensure the safety of the food produced.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100327
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