Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.
In the present study, the effects of two CaCl2.2H2O levels (440 and 1320 mg L-1) and two subcultures were evaluated on in vitro shoots of Lavandula angustifolia cv. Provence Blue. Ca2+ content of the apical, middle and basal portion of shoots was determined. Increasing CaCl2.2H2O level in the culture medium increased tissue Ca2+ content and decreased hyperhydricity. Shoot-tip necrosis also decreased with 1320 mg L-1 CaCl2.2H2O, but it did not occur in the second subculture. The middle and basal portion had higher Ca2+ content than apical portion. In non-hyperhydric tissues, there were smaller and more juxtaposed cells. Scanning electron microscopy of the leaves demonstrated that trichomes from in vitro leaf surface occurred in smaller quantities.
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Instituto de Tecnologia do Paraná - Tecpar
2014
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oai:scielo:S1516-891320140005006362015-10-08Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.Machado,Marília PereiraSilva,André Luís Lopes daBiasi,Luiz AntonioDeschamps,CíceroBespalhok Filho,João CarlosZanette,Flávio True lavender Micropropagation CaCl2.2H2O, Leaf anatomy Ultrastructure Trichome In the present study, the effects of two CaCl2.2H2O levels (440 and 1320 mg L-1) and two subcultures were evaluated on in vitro shoots of Lavandula angustifolia cv. Provence Blue. Ca2+ content of the apical, middle and basal portion of shoots was determined. Increasing CaCl2.2H2O level in the culture medium increased tissue Ca2+ content and decreased hyperhydricity. Shoot-tip necrosis also decreased with 1320 mg L-1 CaCl2.2H2O, but it did not occur in the second subculture. The middle and basal portion had higher Ca2+ content than apical portion. In non-hyperhydric tissues, there were smaller and more juxtaposed cells. Scanning electron microscopy of the leaves demonstrated that trichomes from in vitro leaf surface occurred in smaller quantities.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.57 n.5 20142014-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500636en10.1590/S1516-8913201402165 |
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Machado,Marília Pereira Silva,André Luís Lopes da Biasi,Luiz Antonio Deschamps,Cícero Bespalhok Filho,João Carlos Zanette,Flávio |
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Machado,Marília Pereira Silva,André Luís Lopes da Biasi,Luiz Antonio Deschamps,Cícero Bespalhok Filho,João Carlos Zanette,Flávio Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill. |
author_facet |
Machado,Marília Pereira Silva,André Luís Lopes da Biasi,Luiz Antonio Deschamps,Cícero Bespalhok Filho,João Carlos Zanette,Flávio |
author_sort |
Machado,Marília Pereira |
title |
Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill. |
title_short |
Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill. |
title_full |
Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill. |
title_fullStr |
Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill. |
title_full_unstemmed |
Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill. |
title_sort |
influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of lavandula angustifolia mill. |
description |
In the present study, the effects of two CaCl2.2H2O levels (440 and 1320 mg L-1) and two subcultures were evaluated on in vitro shoots of Lavandula angustifolia cv. Provence Blue. Ca2+ content of the apical, middle and basal portion of shoots was determined. Increasing CaCl2.2H2O level in the culture medium increased tissue Ca2+ content and decreased hyperhydricity. Shoot-tip necrosis also decreased with 1320 mg L-1 CaCl2.2H2O, but it did not occur in the second subculture. The middle and basal portion had higher Ca2+ content than apical portion. In non-hyperhydric tissues, there were smaller and more juxtaposed cells. Scanning electron microscopy of the leaves demonstrated that trichomes from in vitro leaf surface occurred in smaller quantities. |
publisher |
Instituto de Tecnologia do Paraná - Tecpar |
publishDate |
2014 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500636 |
work_keys_str_mv |
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