Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species

Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species

The aim of this study was to evaluate the selected 41 SSR markers developed for yellow passion fruit (Passiflora edulis f. flavicarpa Sims.) for their transferability to 11 different Passiflora species. Twenty-one SSR were successfully amplified in 10 wild species of passion fruit producing 101 bands. All the markers were amplifiable for at least one species. The mean transferability was 68,8%, ranging from 15,4% (primer PE11) to 100 % (PE13, PE18, PE37, PE41 and PE88). Transferability was higher for the species from the Passiflora subgenus than for those from the Decaloba and Dysosmia subgenus. The results indicated a high level of nucleotide sequence conservation of the primer regions in the species evaluated, and consequently, they could potentially be used for the establishment of molecular strategies for use in passion fruit breeding and genetics.

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Main Authors: Oliveira,Gilmara Alvarenga Fachardo, Pádua,Juliano Gomes, Costa,Juliana Leles, Jesus,Onildo Nunes de, Carvalho,Fabiana Moraes de, Oliveira,Eder Jorge de
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2013
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500009
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spelling oai:scielo:S1516-891320130005000092013-10-31Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora SpeciesOliveira,Gilmara Alvarenga FachardoPádua,Juliano GomesCosta,Juliana LelesJesus,Onildo Nunes deCarvalho,Fabiana Moraes deOliveira,Eder Jorge de Transferability SSR passion fruit The aim of this study was to evaluate the selected 41 SSR markers developed for yellow passion fruit (Passiflora edulis f. flavicarpa Sims.) for their transferability to 11 different Passiflora species. Twenty-one SSR were successfully amplified in 10 wild species of passion fruit producing 101 bands. All the markers were amplifiable for at least one species. The mean transferability was 68,8%, ranging from 15,4% (primer PE11) to 100 % (PE13, PE18, PE37, PE41 and PE88). Transferability was higher for the species from the Passiflora subgenus than for those from the Decaloba and Dysosmia subgenus. The results indicated a high level of nucleotide sequence conservation of the primer regions in the species evaluated, and consequently, they could potentially be used for the establishment of molecular strategies for use in passion fruit breeding and genetics.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.56 n.5 20132013-10-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500009en10.1590/S1516-89132013000500009
institution SCIELO
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country Brasil
countrycode BR
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access En linea
databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Oliveira,Gilmara Alvarenga Fachardo
Pádua,Juliano Gomes
Costa,Juliana Leles
Jesus,Onildo Nunes de
Carvalho,Fabiana Moraes de
Oliveira,Eder Jorge de
spellingShingle Oliveira,Gilmara Alvarenga Fachardo
Pádua,Juliano Gomes
Costa,Juliana Leles
Jesus,Onildo Nunes de
Carvalho,Fabiana Moraes de
Oliveira,Eder Jorge de
Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species
author_facet Oliveira,Gilmara Alvarenga Fachardo
Pádua,Juliano Gomes
Costa,Juliana Leles
Jesus,Onildo Nunes de
Carvalho,Fabiana Moraes de
Oliveira,Eder Jorge de
author_sort Oliveira,Gilmara Alvarenga Fachardo
title Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species
title_short Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species
title_full Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species
title_fullStr Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species
title_full_unstemmed Cross-species amplification of microsatellite loci developed for Passiflora edulis Sims. in related Passiflora Species
title_sort cross-species amplification of microsatellite loci developed for passiflora edulis sims. in related passiflora species
description The aim of this study was to evaluate the selected 41 SSR markers developed for yellow passion fruit (Passiflora edulis f. flavicarpa Sims.) for their transferability to 11 different Passiflora species. Twenty-one SSR were successfully amplified in 10 wild species of passion fruit producing 101 bands. All the markers were amplifiable for at least one species. The mean transferability was 68,8%, ranging from 15,4% (primer PE11) to 100 % (PE13, PE18, PE37, PE41 and PE88). Transferability was higher for the species from the Passiflora subgenus than for those from the Decaloba and Dysosmia subgenus. The results indicated a high level of nucleotide sequence conservation of the primer regions in the species evaluated, and consequently, they could potentially be used for the establishment of molecular strategies for use in passion fruit breeding and genetics.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 2013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500009
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