Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil
The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture, which allowed isolating and identifying the parasite and - with the PCR technique - it was possible to diagnose 100% MCL patients for all the three repetitions of exams. The PCR optimized for the present work proved to be an auxiliary method for diagnosing leishmaniasis applicable in the patients carrying MCL due to Leishmania (Viannia) braziliensis and did not need culture to be performed, resulting in a faster diagnosis.
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Instituto de Tecnologia do Paraná - Tecpar
2011
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oai:scielo:S1516-891320110003000082011-06-13Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, BrazilThomaz-Soccol,AndréaMocellin,MarcosMulinari,FabianeCastro,Edilene Alcântara deQueiroz-Telles,Flávio deAlcântara,Fernanda de SouzaBavaresco,Marcello TortelliHennig,LucianeAndraus,AnieloLuz,EnnioThomaz-Soccol,Vanete Mucocutaneous leishmaniasis molecular diagnosis Leishmania (Viannia) braziliensis The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture, which allowed isolating and identifying the parasite and - with the PCR technique - it was possible to diagnose 100% MCL patients for all the three repetitions of exams. The PCR optimized for the present work proved to be an auxiliary method for diagnosing leishmaniasis applicable in the patients carrying MCL due to Leishmania (Viannia) braziliensis and did not need culture to be performed, resulting in a faster diagnosis.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.54 n.3 20112011-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300008en10.1590/S1516-89132011000300008 |
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Thomaz-Soccol,Andréa Mocellin,Marcos Mulinari,Fabiane Castro,Edilene Alcântara de Queiroz-Telles,Flávio de Alcântara,Fernanda de Souza Bavaresco,Marcello Tortelli Hennig,Luciane Andraus,Anielo Luz,Ennio Thomaz-Soccol,Vanete |
| spellingShingle |
Thomaz-Soccol,Andréa Mocellin,Marcos Mulinari,Fabiane Castro,Edilene Alcântara de Queiroz-Telles,Flávio de Alcântara,Fernanda de Souza Bavaresco,Marcello Tortelli Hennig,Luciane Andraus,Anielo Luz,Ennio Thomaz-Soccol,Vanete Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| author_facet |
Thomaz-Soccol,Andréa Mocellin,Marcos Mulinari,Fabiane Castro,Edilene Alcântara de Queiroz-Telles,Flávio de Alcântara,Fernanda de Souza Bavaresco,Marcello Tortelli Hennig,Luciane Andraus,Anielo Luz,Ennio Thomaz-Soccol,Vanete |
| author_sort |
Thomaz-Soccol,Andréa |
| title |
Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| title_short |
Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| title_full |
Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| title_fullStr |
Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| title_full_unstemmed |
Clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Paraná, Brazil |
| title_sort |
clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in paraná, brazil |
| description |
The aim of the present work was to study the clinical aspects and relevance of molecular diagnosis in late mucocutaneous leishmaniasis patients in Parana, Brazil. Twenty one suspected cases of mucocutaneous leishmaniasis (MCL) in patients from the endemic areas of leishmaniasis were assessed. Different methods used in diagnosing the disease and the polymerase chain reaction (PCR) technique were compared in order to establish the sensitivity of each method. Out of the 21 patients analyzed, 14.3% presented other etiologies such as vasculitis, syphilis, and paracoccidioidomycosis, with all tests negative for leishmaniasis. Out of the remaining 15 patients, 6.7% cases were confirmed for leishmaniasis by direct examination; 46.67% were positive for culture, which allowed isolating and identifying the parasite and - with the PCR technique - it was possible to diagnose 100% MCL patients for all the three repetitions of exams. The PCR optimized for the present work proved to be an auxiliary method for diagnosing leishmaniasis applicable in the patients carrying MCL due to Leishmania (Viannia) braziliensis and did not need culture to be performed, resulting in a faster diagnosis. |
| publisher |
Instituto de Tecnologia do Paraná - Tecpar |
| publishDate |
2011 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300008 |
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