Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces

The aim of this study was to characterize bacteriocins produced by 70 strains of Enterococcus mundtii.Four strains exhibited antibiotic activity towards Listeria innocua, L. monocytogenes, Lactobacillus plantarum, and Salmonella Enteritidis. They remained active under temperatures of up to 121ºC for 20 min, and under pH treatments that varied from 2.0 to 10.0. Antimicrobial activity was maintained during the storage test for 60 days under freezing. The kinetics of production revealed the peak activity of 1600 AU /mL during the logarithmic growth phase and the molecular weight found was approximately 3.0 kDa. The characterization of the products with antimicrobial activity indicated their proteic nature, presenting a typical kinetics of primary metabolite and a molecular weight similar to many purified enterocins.

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Main Authors: Ferreira,Alessandra Einsfeld, Canal,Natália, Morales,Daiana, Fuentefria,Daiane Bopp, Corção,Gertrudes
Format: Digital revista
Language:English
Published: Instituto de Tecnologia do Paraná - Tecpar 2007
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200010
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spelling oai:scielo:S1516-891320070002000102007-06-12Characterization of enterocins produced by Enterococcus mundtii isolated from humans fecesFerreira,Alessandra EinsfeldCanal,NatáliaMorales,DaianaFuentefria,Daiane BoppCorção,Gertrudes Enterococcus mundtii enterocins antimicrobial activity biopreservative The aim of this study was to characterize bacteriocins produced by 70 strains of Enterococcus mundtii.Four strains exhibited antibiotic activity towards Listeria innocua, L. monocytogenes, Lactobacillus plantarum, and Salmonella Enteritidis. They remained active under temperatures of up to 121ºC for 20 min, and under pH treatments that varied from 2.0 to 10.0. Antimicrobial activity was maintained during the storage test for 60 days under freezing. The kinetics of production revealed the peak activity of 1600 AU /mL during the logarithmic growth phase and the molecular weight found was approximately 3.0 kDa. The characterization of the products with antimicrobial activity indicated their proteic nature, presenting a typical kinetics of primary metabolite and a molecular weight similar to many purified enterocins.info:eu-repo/semantics/openAccessInstituto de Tecnologia do Paraná - TecparBrazilian Archives of Biology and Technology v.50 n.2 20072007-03-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200010en10.1590/S1516-89132007000200010
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country Brasil
countrycode BR
component Revista
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databasecode rev-scielo-br
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region America del Sur
libraryname SciELO
language English
format Digital
author Ferreira,Alessandra Einsfeld
Canal,Natália
Morales,Daiana
Fuentefria,Daiane Bopp
Corção,Gertrudes
spellingShingle Ferreira,Alessandra Einsfeld
Canal,Natália
Morales,Daiana
Fuentefria,Daiane Bopp
Corção,Gertrudes
Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces
author_facet Ferreira,Alessandra Einsfeld
Canal,Natália
Morales,Daiana
Fuentefria,Daiane Bopp
Corção,Gertrudes
author_sort Ferreira,Alessandra Einsfeld
title Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces
title_short Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces
title_full Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces
title_fullStr Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces
title_full_unstemmed Characterization of enterocins produced by Enterococcus mundtii isolated from humans feces
title_sort characterization of enterocins produced by enterococcus mundtii isolated from humans feces
description The aim of this study was to characterize bacteriocins produced by 70 strains of Enterococcus mundtii.Four strains exhibited antibiotic activity towards Listeria innocua, L. monocytogenes, Lactobacillus plantarum, and Salmonella Enteritidis. They remained active under temperatures of up to 121ºC for 20 min, and under pH treatments that varied from 2.0 to 10.0. Antimicrobial activity was maintained during the storage test for 60 days under freezing. The kinetics of production revealed the peak activity of 1600 AU /mL during the logarithmic growth phase and the molecular weight found was approximately 3.0 kDa. The characterization of the products with antimicrobial activity indicated their proteic nature, presenting a typical kinetics of primary metabolite and a molecular weight similar to many purified enterocins.
publisher Instituto de Tecnologia do Paraná - Tecpar
publishDate 2007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200010
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AT canalnatalia characterizationofenterocinsproducedbyenterococcusmundtiiisolatedfromhumansfeces
AT moralesdaiana characterizationofenterocinsproducedbyenterococcusmundtiiisolatedfromhumansfeces
AT fuentefriadaianebopp characterizationofenterocinsproducedbyenterococcusmundtiiisolatedfromhumansfeces
AT corcaogertrudes characterizationofenterocinsproducedbyenterococcusmundtiiisolatedfromhumansfeces
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